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70 μm pore sized cell strainers

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The 70-μm pore-sized cell strainers are a type of laboratory equipment used for filtering and separating cells or other biological particles. They feature a mesh with a pore size of 70 micrometers, which is designed to retain larger cells or cell aggregates while allowing smaller particles to pass through.

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Lab products found in correlation

Sytox blue, by Thermo Fisher Scientific (3 mentions) Lsrii flow cytometer, by BD (3 mentions) Anti cd11b pe cy7, by BD (3 mentions) Facsdiva, by BD (2 mentions) Pacific blue anti ly6g, by BD (2 mentions) Anti cd11c apc, by BD (1 mentions) Easysep mouse hematopoietic progenitor cell isolation kit, by STEMCELL (1 mentions) Easysep mouse t cell isolation kit, by STEMCELL (1 mentions) Anti biotin microbead, by Miltenyi Biotec (1 mentions) Anti sca 1, by Thermo Fisher Scientific (1 mentions) Anti gr 1 apc, by BD (1 mentions) Anti cd150, by Thermo Fisher Scientific (1 mentions) Anti c kit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated anti cd8a, by BioLegend (1 mentions) Percp cy5.5 conjugated anti gr 1, by BioLegend (1 mentions) Fitc conjugated anti cd11b, by Thermo Fisher Scientific (1 mentions) Cell activation cocktail including brefeldin a, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Hematocytometer, by Hausser Scientific (1 mentions) Pe anti f4 80, by BD (1 mentions)

Close spelling variants of this product

70-μm cell strainer (905 citations) Cell strainer (717 citations) 70-μm strainer (108 citations) μm cell strainers (7 citations) 70 μm-pore cell strainer (6 citations)

6 protocols using 70 μm pore sized cell strainers

1

Characterization of Splenic Immune Cells

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Spleens were harvested from naive mice and septic mice at days 7 and 14. Single-cell suspensions were created by passing cells through 70-μm pore-sized cell strainers (BD Falcon, Durham, NC). Erythrocytes were then lysed using ammonium chloride lysis buffer and washed twice using phosphate-buffered saline. Cells were stained with the following antibodies for flow cytometric studies: PE Cy7 anti-CD11b, PB anti-Ly6C, APC Cy7 anti-Ly6G, PerCpCy5.5 anti-MHCII, FITC anti-F4/80, and APC anti-CD11c (BD Pharmigen, Billerica, MA). Sytox Blue (Invitrogen, Carlsbad, CA) was used for cell viability analysis, and samples were acquired and analyzed using a LSRII flow cytometer (BD Biosciences) and FACSDiva (BD Biosciences, San Jose, CA).
Stortz J.A., Hollen M.K., Nacionales D.C., Horiguchi H., Ungaro R., Dirain M.L., Wang Z., Wu Q., Wu K.K., Kumar A., Foster T.C., Stewart B.D., Ross J.A., Segal M., Bihorac A., Brakenridge S., Moore F.A., Wohlgemuth S.E., Leeuwenburgh C., Mohr A.M., Moldawer L.L, & Efron P.A. (2019). Old Mice Demonstrate Organ Dysfunction as well as Prolonged Inflammation, Immunosuppression, and Weight Loss in a Modified Surgical Sepsis Model. Critical care medicine, 47(11), e919-e929.
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2

Isolating Hematopoietic Stem Cells from Aged Mice

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Bone marrow cells from young and old adult mice were aseptically collected from the non-injured lower extremity (femur bone) 1 day after polytrauma (old PT n=8, young PT n=8) or 1 day after pneumonia (old PT+PNA n=7, young PT+PNA n=7), as well as from naïve young and old adult control mice. Single cell suspensions were created by passing the cells through 70 μm pore sized cell strainers (BD Falcon, Durham, NC). Erythrocytes were lysed using ammonium chloride lysis buffer and washed with phosphate-buffered saline. HSPCs were isolated via immunomagnetic negative selection using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation kit (StemCell Technologies, Vancouver, BC) according to the manufacturer’s protocol (9 (link)).
Darden D.B., Mira J.C., Lopez M.C., Stortz J.A., Fenner B.P., Kelly L.S., Nacionales D.C., Budharaju A., Loftus T.J., Baker H.V., Moore F.A., Brakenridge S.C., Moldawer L.L., Mohr A.M, & Efron P.A. (2021). Identification of Unique microRNA Expression Patterns in Bone Marrow Hematopoietic Stem and Progenitor Cells after Hemorrhagic Shock and Polytrauma in Young and Old Adult Mice. The journal of trauma and acute care surgery, 91(4), 692-699.
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3

Immunomodulatory Effects of MDSCs on T Cell Proliferation

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Similar to phenotyping, spleens were harvested from septic and nonseptic mice, and single cell suspensions were collected by passing the cells through 70-μm pore-sized cell strainers (BD Falcon, Durham, NC). T cells were collected by negative immunomagnetic selection using EasySep Mouse T Cell Isolation Kit (Stemcell Technologies, Vancouver, BC, Canada). MDSCs were enriched by positive immunomagnetic separation using anti-Gr-1 biotin followed by anti-Biotin microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). T cells were stained with cell trace violet to detect cell proliferation then plated with and without MDSCs in a 1:1 ratio, stimulated with anti-CD3+/CD28+ plate-bound antibodies at a concentration of 1 μg/mL, and allowed to incubate for 4 days at 37°C. T cell proliferation was then measured in the presence and absence of MDSCs. The proliferation index and percent CD4+ and CD8+ T cell suppression were calculated for every murine subject at each time point.
Stortz J.A., Hollen M.K., Nacionales D.C., Horiguchi H., Ungaro R., Dirain M.L., Wang Z., Wu Q., Wu K.K., Kumar A., Foster T.C., Stewart B.D., Ross J.A., Segal M., Bihorac A., Brakenridge S., Moore F.A., Wohlgemuth S.E., Leeuwenburgh C., Mohr A.M., Moldawer L.L, & Efron P.A. (2019). Old Mice Demonstrate Organ Dysfunction as well as Prolonged Inflammation, Immunosuppression, and Weight Loss in a Modified Surgical Sepsis Model. Critical care medicine, 47(11), e919-e929.
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4

Multiparametric Flow Cytometry Analysis

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Spleen, blood, bone marrow (BM) and BAL cells were harvested and single cell suspensions were created by passing the cells through 70 μm pore sized cell strainers (BD Falcon, Durham, NC). Erythrocytes were then lysed using ammonium chloride lysis buffer and washed two times using phosphate-buffered saline (PBS) without calcium, phenol red, or magnesium. Cells were stained with the following antibodies for flow cytometric studies: PE Cy7 anti-CD11b, APC anti-Gr-1, and Pacific Blue anti-Ly6G (BD Pharmingen, Billerica, MA). Additional antibodies used were anti-lineage mixture (Lin; BD Biosciences, San Jose, CA), anti-ckit, anti-Sca-1, anti-CD135, anti-CD150 (eBioscience, San Diego, CA). Sytox Blue (Invitrogen, Carlsbad, CA) was used for cell viability analysis and samples were acquired and analyzed using an LSRII flow cytometer (BD Biosciences) and FACSDiva™ (BD Biosciences)(37 (link), 38 (link)).
Nacionales D.C., Szpila B., Ungaro R., Lopez M.C., Zhang J., Gentile L.F., Cuenca A.L., Vanzant E., Mathias B., Jyot J., Westerveld D., Bihorac A., Joseph A., Mohr A., Duckworth L.V., Moore F.A., Baker H.V., Leeuwenburgh C., Moldawer L.L., Brakenridge S, & Efron P.A. (2015). A Detailed Characterization of the Dysfunctional Immunity and Abnormal Myelopoiesis Induced by Severe Shock and Trauma in the Aged. Journal of immunology (Baltimore, Md. : 1950), 195(5), 2396-2407.
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5

Multicolor Flow Cytometry Analysis of Immune Cell Subsets

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Spleen cells were harvested by passing the single-cell suspensions through 70-μm pore-sized cell strainers (BD Falcon, Durham, NC). For detection of myeloid-derived suppressor cells (MDSCs), splenocytes were stained with FITC-conjugated anti-CD11b (eBioscience), percpcy5.5-conjugated anti-Gr-1 (Biolegend, San Diego, CA), and PE-CY7 anti-CD244 (Biolegend) antibodies. To examine the expression of INF-γ and CD107a in CD8 + T cells, both CD8 + T lymphocytes from the in vitro co-culture and the isolated splenocytes were stimulated with a cell activation cocktail (including Brefeldin A; Biolegend) according to the manufacturer's instructions and incubated at 37°C for 6 h before staining. The cells were stained with Brilliant Violet 510-conjugated anti-CD3 (Biolegend) and Alexa Fluor 488-conjugated anti-CD8a (Biolegend), followed by intracellular staining with APC-conjugated anti-interferon γ (anti-IFN-γ; eBioscience) and PE-conjugated anti-CD107a (eBioscience). To examine the expression of programmed death 1 (PD-1) in CD8 + T lymphocytes in vitro, the cells were stained with Alexa Fluor 488-conjugated anti-CD8a and Brilliant Violet 421-conjugated anti-CD279 (PD-1; Biolegend). Cells were analyzed using an LSR Fortessa (BD Biosciences, San Jose, CA) flow cytometer, and the data were analyzed using FlowJo software (BD Life Sciences, Franklin Lakes, NJ).
Chen X., Chen M., Yang Y., Xu C., Lu H., Xu Y., Li X., Wei Y., Zhu Z., Ding Y, & Yu W. (2022). LIPOPOLYSACCHARIDE-PRECONDITIONED MESENCHYMAL STEM CELL TRANSPLANTATION ATTENUATES CRITICAL PERSISTENT INFLAMMATION IMMUNE SUPPRESSION AND CATABOLISM SYNDROME IN MICE. Shock (Augusta, Ga.), 58(5).
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6

Bronchoalveolar Lavage and Flow Cytometry

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The trachea was cannulated and lavaged four times with 800 μl cold PBS containing 2 mM EDTA. The BAL cells were counted using a hematocytometer (Hausser Scientific, Horsham, PA). BAL single cell suspensions were created by passing the cells through 70 μm pore sized cell strainers (BD Falcon, Durham, NC). Cells were stained with the following antibodies for flow cytometric studies: PE Cy7 anti-CD11b, APC anti-siglec, and Pacific Blue anti-Ly6G, FITC anti CD11c, and PE anti-F4/80 (BD Pharmingen, Billerica, MA). Sytox Blue (Invitrogen, Carlsbad, CA) was used for cell viability analysis and samples were acquired and analyzed using an LSRII flow cytometer (BD Biosciences) and FACSDiva™.
Darden D.B., Mira J.C., Lopez M.C., Stortz J.A., Fenner B.P., Kelly L.S., Nacionales D.C., Budharaju A., Loftus T.J., Baker H.V., Moore F.A., Brakenridge S.C., Moldawer L.L., Mohr A.M, & Efron P.A. (2021). Identification of Unique microRNA Expression Patterns in Bone Marrow Hematopoietic Stem and Progenitor Cells after Hemorrhagic Shock and Polytrauma in Young and Old Adult Mice. The journal of trauma and acute care surgery, 91(4), 692-699.
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