Analytical grade calcium nitrate Ca(NO3)2·4H2O, diammonium hydrogen phosphate (NH4)2HPO4, silver nitrate Ag(NO3), gadolinium oxide Gd2O3, ferric nitrate nonahydrate Fe(NO3)3·9H2O, and ammonia (30% GR) were purchased from MERCK, India. The drug tetracycline was purchased from Sigma Aldrich, India.
Diammonium hydrogen phosphate nh4 2hpo4
Manufactured by Merck Group
Sourced in United States, India
Diammonium hydrogen phosphate ((NH4)2HPO4) is a chemical compound that is commonly used in laboratory settings. It is a white, crystalline solid that is soluble in water. The compound is composed of ammonium (NH4+) and hydrogen phosphate (HPO4²-) ions. Diammonium hydrogen phosphate serves as a source of phosphate ions and is often used in buffer solutions, as a nutrient in microbiological media, and as a pH regulator.
Automatically generated - may contain errors
Lab products found in correlation
Calcium nitrate tetrahydrate, by Merck Group (5 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Manganese nitrate tetrahydrate, by Merck Group (2 mentions)
Tetracycline, by Merck Group (1 mentions)
Calcium nitrate ca no3 2 4h2o, by Merck Group (1 mentions)
Silver nitrate agno3, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Sodium hydrogen carbonate, by Merck Group (1 mentions)
Ammonium cerium 4 nitrate nh4 2ce no3 6, by Merck Group (1 mentions)
β glucuronidase from e coli k12, by Roche (1 mentions)
Lc ms grade methanol, by Scharlab (1 mentions)
Milli q system, by Merck Group (1 mentions)
Acetone, by Scharlab (1 mentions)
Hydrochloric acid (hcl), by Scharlab (1 mentions)
Thiazolyl blue tetrazolium bromide, by Thermo Fisher Scientific (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
C2h5oh, by Merck Group (1 mentions)
12 protocols using diammonium hydrogen phosphate nh4 2hpo4
1
Synthesis of Inorganic Compounds
2
Synthesis of β-TCP Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We followed the earlier recommendations of Mehdikhani and Borhani [21 (link)] to synthesize β-TCP nanoparticles. Briefly, calcium nitrate tetra-hydrate ((Ca(NO3)2·4H2O) (98%, Merck, Kenilwork, NJ, USA) and diammonium hydrogen phosphate ((NH4)2HPO4) (99%, Merck) were reacted to produce β-TCP nanoparticles. Initially, a white precipitate was produced by adding 500 mL of 0.6 mol Ca(NO3)2 (pH = 7.3) dropwise over a time period of 2–3 h in 500 mL of 0.4 mol (NH4)2HPO4 solution (pH = 4) that was robustly agitated before the mixing. During this reaction, aliquots of 0.1 M sodium hydroxide (99%, Merck) were used to maintain the pH of the system at 8. The resultant white precipitate was first stirred for 12 h and then washed with distilled water (DW) and ethanol to augment its dispersion features. Utilizing mild suction, this mixture was filtered inside a filter glass. Post-filtration drying of the filtration cake was carried out at 80 °C for one day. On the next day, the dried powders were obtained and broken down using a mortar and pestle and calcined inside an alumina crucible at 700 °C temperature for 2 h.
3
Synthesis of Cellulose Nanofibrils from LSSU Shells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LSSU shells used as the Ca source were collected from Buleleng, Bali, Indonesia. Diammonium hydrogen phosphate ((NH4)2HPO4) used as a phosphate source, and sodium hydrogen carbonate (NaHCO3) as a carbonate source were purchased from Merck (USA). The PVA with a molecular weight of 145 000 (100% hydrolysed) was purchased from Merck (Germany). The MCC was purchased from Sigma-Aldrich (USA) and used as the precursor for cellulose nanofibrils.
4
Composite Scaffolds from Mineral Powders and Bioresorbable Polymer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The purpose of this work resides in the obtaining of composite scaffolds based on mineral powders and a bioresorbable polymer. To attain it, the following reagents were employed: ammonium cerium(IV) nitrate ((NH4)2Ce(NO3)6, ≥98%, Sigma-Aldrich, Burlington, MA, USA), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O, 99–102%, Merck, Darmstadt, Germany), diammonium hydrogen phosphate ((NH4)2HPO4, ≥99%, Merck, Darmstadt, Germany), ammonium hydroxide (NH4OH, 25% NH3, Sigma-Aldrich, Burlington, MA, USA), tetraethyl orthosilicate (Si(OC2H5)4, TEOS, 98%, Aldrich, Burlington, MA, USA), triethyl phosphate ((C2H5O)3PO, TEP, 99%, Merck, Darmstadt, Germany), sodium nitrite (NaNO2, 99%, Riedel-de Haën, Charlotte, NC, USA), nitric acid (HNO3, ≥65%, Fluka, Charlotte, NC, USA), polycaprolactone ((C6H10O2)n, PCL, Mw = 80,000 g/mol, Sigma-Aldich, Burlington, MA, USA), chloroform (CHCl3, CF, ≥99%, Sigma-Aldich, Burlington, MA, USA) and N,N-dimethylformamide (C3H7NO, DMF, 99.8%, Sigma-Aldich, Sigma-Aldich, Burlington, MA, USA).
5
Quantitative HPLC-MS Workflow Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC-grade water was obtained by purifying demineralised water using a Milli-Q system from Millipore (Bedford, MA, USA). LC-MS grade methanol, acetone, and hydrochloric acid (HCl 37%) were purchased from Scharlau (Scharlab, Barcelona, Spain).
Diammonium hydrogen phosphate ((NH4)2HPO4) was purchased from Merck (Darmstadt, Germany). β-glucuronidase from E. Coli K12 (80 U/mL at 25 ºC) was purchased from Roche (Indianapolis, IN, USA).
1 M H2PO4 -/HPO4 -2 buffer was prepared by dissolving the corresponding amount of (NH4)2HPO4 in HPLC-grade water and adjusting the pH to 7 with HCl.
Diammonium hydrogen phosphate ((NH4)2HPO4) was purchased from Merck (Darmstadt, Germany). β-glucuronidase from E. Coli K12 (80 U/mL at 25 ºC) was purchased from Roche (Indianapolis, IN, USA).
1 M H2PO4 -/HPO4 -2 buffer was prepared by dissolving the corresponding amount of (NH4)2HPO4 in HPLC-grade water and adjusting the pH to 7 with HCl.
6
Synthesis of Iron-Phosphate-Silica Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and solvents were of analytical grade and used without further purification. Iron(iii ) chloride hexahydrate (FeCl3·6H2O, 97%), tetraethyl orthosilicate (TEOS) and ciprofloxacin (98%) were purchased from Sigma-Aldrich, while di-ammonium hydrogen phosphate ((NH4)2HPO4, 99+%) was procured from Merck and ammonium hydroxide solution (NH4OH, 28–30%) from Fluka. Cell culture materials were obtained by Life Technologies except for paraformaldehyde (PFA) (Merck) and thiazolyl blue tetrazolium bromide (MTT, 98%, Alfa Aesar).
7
Fabrication of PCL Filaments with Calcium Phosphate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pellets of poly-e-caprolactone (PCL, MW 80,000. Sigma-Aldrich) were used as raw material in the fabrication of filaments. A mixture of ethanol (C2H5OH, Merck), dichloromethane (CH2Cl2, Loba Chemie), and tetrahydrofuran (C4H8O, Merck) was used to dissolve the PCL. Calcium nitrate tetrahydrate (Ca(NO3)2.4(H2O), Merck), diammonium hydrogen phosphate ((NH4)2HPO4, Scharlau), nitric acid (HNO3, Merck), and glycine (C2H5NO2 Gly, Aesar Alfa) were used to obtain calcium phosphates nanoparticles [16 (link),26 (link)].
8
Synthesis of Tin-Functionalized Hydroxyapatite
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aqueous solutions of calcium nitrate tetrahydrate, Ca(NO3)2·4H2O (> 99.0%, Merck ACS) and diammonium hydrogen phosphate, (NH4)2HPO4 (> 98.0%, Sigma-Aldrich) were used as precursors for the synthesis of hydroxyapatite, ammonium hydroxide solution, NH4OH (28–30 wt%, Fluka) was used for pH adjustment during the synthesis.
Tin chloride dihydrate (SnCl2·2H2O, ≥ 98.0% oxidimetric assay), potassium bichromate (K2Cr2O7, ≥ 99.0% oxidimetric assay), calcium nitrate tetrahydrate, (Ca(NO3)2·4H2O, > 99.0%, ACS), magnesium sulfate anhydrous (MgSO4, 99%) and zinc nitrate hexahydrate (Zn(NO3)2·6H2O, for analysis) were purchased from Carlo Erba. Calcium chloride (CaCl2, ≥ 99.99%), sodium sulfate decahydrate (NaSO4·10H2O, ≥ 99.0%, ACS Reagent), iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O, ≥ 99.95%) and manganese nitrate tetrahydrate (Mn(NO3)2·4H2O, ≥ 97.0%) salts were purchased from Sigma-Aldrich. Aluminum chloride hexahydrate (AlCl3·6H2O, 99%) was from Fluka. Hydrochloric acid (37 wt%) was from Merck. Nitrogen, 99.9995% purity from SAPIO was used as inert gas. All the solutions were prepared using MilliQ water (ρ ≥ 17.5 MΩ cm; TOC, 2 ppb).
Tin-functionalized hydroxyapatite (Sn/HAP, 10 wt% Sn loading) was prepared from an acidic tin chloride solution (pH ~ 2) by using a flash deposition technique already validated to deposit Sn2+ species onto HAP31 (link).
Tin chloride dihydrate (SnCl2·2H2O, ≥ 98.0% oxidimetric assay), potassium bichromate (K2Cr2O7, ≥ 99.0% oxidimetric assay), calcium nitrate tetrahydrate, (Ca(NO3)2·4H2O, > 99.0%, ACS), magnesium sulfate anhydrous (MgSO4, 99%) and zinc nitrate hexahydrate (Zn(NO3)2·6H2O, for analysis) were purchased from Carlo Erba. Calcium chloride (CaCl2, ≥ 99.99%), sodium sulfate decahydrate (NaSO4·10H2O, ≥ 99.0%, ACS Reagent), iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O, ≥ 99.95%) and manganese nitrate tetrahydrate (Mn(NO3)2·4H2O, ≥ 97.0%) salts were purchased from Sigma-Aldrich. Aluminum chloride hexahydrate (AlCl3·6H2O, 99%) was from Fluka. Hydrochloric acid (37 wt%) was from Merck. Nitrogen, 99.9995% purity from SAPIO was used as inert gas. All the solutions were prepared using MilliQ water (ρ ≥ 17.5 MΩ cm; TOC, 2 ppb).
Tin-functionalized hydroxyapatite (Sn/HAP, 10 wt% Sn loading) was prepared from an acidic tin chloride solution (pH ~ 2) by using a flash deposition technique already validated to deposit Sn2+ species onto HAP31 (link).
9
Synthesis of Chitosan-Coated Mg-Doped Hydroxyapatite
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The precursors used in order to obtain chitosan-coated magnesium-doped hydroxyapatite (Ca10−xMgx(PO4)6(OH)2; xMg = 0.1; 10 MgHApCh) via sol-gel method were: magnesium nitrate hexahydrate (Mg(NO3)2·6H2O; 99.97 %; Alpha Aesar, Kandel, Germany), calcium nitrate tetrahydrate (Ca(NO3)2∙4H2O, ≥99.0 %, Sigma Aldrich, St. Louis, MO, USA), and diammonium hydrogen phosphate ((NH4)2HPO4; ≥99.0 %, Sigma Aldrich, St. Louis, MO, USA). The ammonium hydroxide (NH4OH, 25% NH3 in H2O (T)), chitosan (C6H11NO4), and ethanol absolute (C2H5OH) purchased from Sigma Aldrich (St. Louis, MO, USA) were also used. Double distilled water was also used for the synthesis of 10 MgHApCh.
10
Synthesis of Zinc-Calcium Phosphate Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc nitrate hexahydrate (Zn(NO3)2·6H2O), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O), diammonium hydrogen phosphate (NH4)2HPO4, manganese nitrate tetrahydrate (Mn(NO3)2·4H2O), ethanol (EtOH), and ammonium hydroxide (NH4OH) were purchased from Sigma Aldrich Chemical & Co. (Tamil Nadu, India). All the chemicals were of analytical grade, and deionized water was used throughout the experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!