Stromal cell basal medium

LNCaP cells, MCF7 cells, MDA-MB-231 cells, Caco-2 cells, HEK293 cells, and Cos7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque, Kyoto, Japan); PC3 cells were cultured in RPMI 1640 medium (Nacalai Tesque); and A375 cells were cultured in Eagle’s Minimum Essential Medium (Wako Pure Chemical Industries, Osaka, Japan). All media were supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Saint Louis, MO, USA), 20 mM l-glutamine (Nacalai Tesque) and 100 µ/mL penicillin–streptomycin (Nacalai Tesque). PrS cells were purchased from Lonza (Cat. No. CC-2508; Basel, Switzerland) and cultured in Stromal cell basal medium supplemented with growth factors (Lonza). Male BALB/c nude mice (7-weeks-old) were purchased from Japan SLC (Shizuoka, Japan). The animal experiments conducted in this study were approved by the Shiga University of Medical Science Animal Care and Use Committee according to the Animal Research Reporting of In Vivo Experiments guidelines..

LNCaP, VCaP and PC3 cells were purchased from ATCC (Manassas, United States). Cells were grown in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with fetal bovine serum (FBS) (BioWest, South American origin), at 10% in 37 °C and 5% CO₂ atmosphere. RWPE-1 cell line was acquired from ATCC and was cultured in Keratinocyte Serum Free Medium (Lonza, Allendale, NJ, USA), PrSC cell line was purchased from LONZA and was cultured in Stromal Cell Basal Medium (LONZA). When cells were treated with DHT hormone, RPMI medium without phenol red (Sigma) supplemented with 5% of charcoal stripped FBS (LONZA) was used.