Pt 31 peltier system

The LCST of the polymers was determined by measuring the optical transmittance in PBS solution (0.5 w/v %) over a temperature range using a UV–VIS spectrophotometer (V-630, Jasco, Tokyo, Japan). The transmittance of the solution was measured at 500 nm. The temperature was controlled using a PT-31 Peltier system (Krüss, Hamburg, Germany) and an ETC-717 controller (Jasco). The heating rate was 0.1 °C/min. The LCST was determined to be the temperature of 50% transmittance of the solution.

The LCST of each polymer was assessed by measuring the optical transmittance of each polymer dissolved in deionized water (5 mg mL⁻¹) at the detection wavelength of 500 nm with an UV-Vis spectrophotometer (V-630, JASCO, Tokyo, Japan). PT-31 Peltier system (Krüss, Hamburg, Germany) and ETC-717 controller (JASCO) were used to control the temperature at a heating rate of 0.1 °C min⁻¹. The LCST of each polymer was defined as the temperature at 50% of optical transmittance.

The P(NIPAAm-co-BMA-co-DMAPAAm) (IBD) linear polymer was synthesized via radical polymerization as described elsewhere [33 (link)]. Briefly, NIPAAm (8.72 g, 77.0 mmol), BMA (0.60 g, 4.20 mmol), and DMAPAAm (0.67 g, 4.31 mmol) were dissolved in N,N-dimethylformamide (DMF) (80 mL), and the solution was then supplemented with 2,2′-azobis(isobutyronitrile) (radical initiator, 0.13 g, 0.81 mmol) and 3-mercaptopropionic acid (chain transfer agent, 0.21 g, 2.00 mmol). After 5-h heating at 70 °C, the solution was repeatedly poured into diethyl ether (300 mL) to precipitate the polymer as a white solid. The NIPAAm/BMA/DMAPAAm molar ratio was 90:5:5 and is hereinafter denoted as IB5D5.
The LCST of the IB5D5 linear polymer was determined from its optical transmittance. Specifically, 5 mg mL⁻¹ solutions of this polymer in each mobile phase employed for analysis were subjected to measurements of optical transmittance at 500 nm using a UV–Vis spectrophotometer (V-630, Jasco, Tokyo, Japan). Solution temperature was controlled by a temperature controller (ETC-717, Jasco) and a PT-31 Peltier system (Krüss, Hamburg, Germany) at a heating rate of 0.1 °C min⁻¹. The LCST was determined as the polymer solution temperature at 50% optical transmittance.