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Mts assay kit

Manufactured by Merck Group
Sourced in United States

The MTS Assay Kit is a colorimetric assay used to measure the metabolic activity of cells. It is based on the reduction of the tetrazolium compound MTS by viable cells, which produces a colored formazan product that can be quantified using a spectrophotometer.

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3 protocols using mts assay kit

1

Cell Proliferation Assay with MTS

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Cell proliferation was detected using MTS Assay Kit (Sigma).
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2

Cell Viability Measurement by MTS Assay

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The viability of LSCC cells was measured by MTS Assay Kit (Sigma, St. Louis, MO, USA). 2 × 103 cells were seeded in 96-well plates, then 20 μl MTS Assay Kit was added to each well and incubated for 2 h at 37 °C. The absorbance was detected using Spark® multimode microplate reader (Mod: SPARK 10 M, TECAN, Switzerland) at a wavelength of 490 nm.
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3

Cell Proliferation and Wound Healing

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Cell proliferation was measured using MTS Assay Kit (Sigma). For cell scratch-wound assays, cells were inoculated in 6-well plates and transfected 12 hours later. A horizontal line was drawn on the monolayers with a 200 µL tip. At 0, 24 and 48 h, they were photographed and analyzed under a microscope.
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