OVA323–339 peptide was synthesized by the Emory University Microchemical Core Facility. Ovalbumin was from Worthington Biochemical Corporation and hen egg lysozyme was from Sigma-Aldrich. Some reagents were purchased: mouse IFN-γ (PeproTech), and lisinopril (Sigma). The PE-conjugated anti-CD69, PE-conjugated anti-CD80 (16–10A1), APC-conjugated anti-CD3ε, APC-conjugated anti-CD86 (GL-1) and the ELISA kits for IL-2, IFN-γ and IL-17A were all purchased from eBioscience. Alexa Fluor 488-conjugated Ovalbumin was from ThermoFisher. AF6–120.1 (anti-I-Ab),1G11 (anti-EEA1) and eBioH4A3 (anti-LAMP-1) were from eBioscience. A rabbit antiserum that recognizes mouse ACE was previously described (13 (link)). The FITC-conjugated mouse TCR Vβ Screening Panel was from BD Pharmingen. Flow cytometry stained samples were analyzed on CyAn ADP (Beckman Coulter) and data were analyzed with FlowJo software.
Pe conjugated anti cd69
Manufactured by Thermo Fisher Scientific
Sourced in United States
PE-conjugated anti-CD69 is a fluorescent-labeled antibody that binds to the CD69 cell surface antigen. CD69 is a early activation marker expressed on various immune cell types. The PE fluorescent dye is conjugated to the antibody, allowing for the detection and analysis of CD69-expressing cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Lisinopril, by Merck Group (2 mentions)
Hen egg lysozyme, by Merck Group (2 mentions)
Mouse ifn γ, by Thermo Fisher Scientific (2 mentions)
Elisa kits for il 2, by Thermo Fisher Scientific (2 mentions)
Pe conjugated anti cd80 16 10a1, by Thermo Fisher Scientific (2 mentions)
Apc conjugated anti cd3ε, by Thermo Fisher Scientific (2 mentions)
Apc conjugated anti cd86 gl 1, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 conjugated ovalbumin, by Thermo Fisher Scientific (2 mentions)
Ebioh4a3 anti lamp 1, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated mouse tcr vβ screening panel, by BD (2 mentions)
Cyan adp, by Beckman Coulter (2 mentions)
Ifn γ, by Thermo Fisher Scientific (2 mentions)
Ovalbumin (ova), by Worthington (2 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Anti myc antibody, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti mouse igg, by Merck Group (1 mentions)
Fitc conjugated anti cd3, by Thermo Fisher Scientific (1 mentions)
Antiphospho iκb α antibody, by Santa Cruz Biotechnology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
4 protocols using pe conjugated anti cd69
1
Ovalbumin Peptide Immunization Protocol
2
Ovalbumin Peptide Immunization Protocol
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OVA323–339 peptide was synthesized by the Emory University Microchemical Core Facility. Ovalbumin was from Worthington Biochemical Corporation and hen egg lysozyme was from Sigma-Aldrich. Some reagents were purchased: mouse IFN-γ (PeproTech), and lisinopril (Sigma). The PE-conjugated anti-CD69, PE-conjugated anti-CD80 (16–10A1), APC-conjugated anti-CD3ε, APC-conjugated anti-CD86 (GL-1) and the ELISA kits for IL-2, IFN-γ and IL-17A were all purchased from eBioscience. Alexa Fluor 488-conjugated Ovalbumin was from ThermoFisher. AF6–120.1 (anti-I-Ab),1G11 (anti-EEA1) and eBioH4A3 (anti-LAMP-1) were from eBioscience. A rabbit antiserum that recognizes mouse ACE was previously described (13 (link)). The FITC-conjugated mouse TCR Vβ Screening Panel was from BD Pharmingen. Flow cytometry stained samples were analyzed on CyAn ADP (Beckman Coulter) and data were analyzed with FlowJo software.
3
HEK293 and Jurkat T Cell Culture
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HEK293 cells were maintained in DMEM supplemented with 5% FBS. Jurkat T cells were maintained in RPMI 1640 supplemented with 5% FBS. Anti-GAPDH antibodies were purchased from Santa Cruz. Anti-Myc antibody was purchased from Cell Signaling. HRP-conjugated anti-mouse IgG and anti-HA antibodies were purchased from Sigma. Anti-human CD3, anti-human CD28, PE-conjugated anti-CD69, and PerCP-conjugated anti-CD25 antibodies were purchased from eBiosciences.
4
Antibody Reagents for T-Cell Activation
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Mouse anti-CD3 (OKT-3) antibody was purchased from e-Bioscience (eBioscience, San Diego, CA, USA). Anti-Nck1, anti-phospho-MEK1/2 and anti-phospho-p38, antibodies were from Cell Signaling (Cell Signaling Technology, Danver, MA, USA). Anti-Nck2 antibody was purchased from Abnova (Abnova Corp., Taipei, Taiwan). Anti-phospho-Erk1/2 antibody was from Upstate (Upstate Biotechnology, Lake Placid, NY, USA). Anti-phospho IκBα antibody was supplied from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz, CA, USA). FITC-conjugated anti-CD3 and PE-conjugated anti-CD69 antibodies were purchased from eBioscience (eBioscience, San Diego, CA, USA).
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