An FDA-approved drug library containing 2,374 compounds (MCE, HY-L022) was screened using LiveArt. All compounds were diluted to 10 µM as the final concentration. Clonal cells with stable 5.8S rRNA tagging were seeded in a 96-well plate prior to drug treatment. After 3 h of drug treatment, imaging was performed on a widefield microscopy equipped with 40× NA 0.75 PlanApo air immersion objective, an LED source (SPECTRA 4), an sCMOS camera (ZYLA 4.2 MP Plus), and a stage incubator (Tokai Hit, STRF-WELSXSET). If the repression of rRNA synthesis was not significant, imaging was performed again to confirm the effects at 24 h after drug treatment. All the candidate drugs were repeated twice.
Zyla 4.2 mp plus
Manufactured by Oxford Instruments
The ZYLA 4.2 MP Plus is a scientific camera designed for a variety of imaging applications. It features a 4.2 megapixel scientific CMOS sensor with a fast readout speed and low noise performance. The camera is capable of capturing high-quality images and videos at a range of resolutions and frame rates.
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Lab products found in correlation
2 protocols using zyla 4.2 mp plus
1
High-throughput Screening of FDA-approved Drugs
2
Widefield Microscopy Imaging Protocol
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All widefield microscopy images were performed on a Nikon Ti2-E fluorescence microscope equipped with a ×100 NA 1.45 PlanApo oil immersion objective, an LED source (SPECTRA 4), an sCMOS camera (ZYLA 4.2MP Plus), a Perfect Focus Unit (Nikon), and a motorized stage (Nikon) with stage incubator (Tokai Hit, STRF-WELSX-SET). Cells were grown in 8-well chambered coverglass for live cell imaging. For live-cell imaging, cells were maintained at 37°C and 5% CO2 in a humidified chamber. The following images were acquired on this widefield microscope: Figure 3D and Supplementary Figures S6, S7, S19, S21 . The following quantifications were analyzed based on widefield images: Figures 2D -E , 3H and Supplementary Figures S1C–D, S6B–C, S7C, S15B–C, S19B, S21B–C, S22D–F .
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