Eclipse 50i light microscope

Manufactured by Nikon

Sourced in Japan

The Eclipse 50i light microscope is a high-performance optical instrument designed for laboratory use. It features a compound optical system that allows for magnification and observation of small-scale samples or specimens. The microscope is equipped with various objective lenses, providing a range of magnification capabilities to suit different research and analysis needs. The Eclipse 50i is a versatile tool for various scientific and educational applications, including microscopy, materials science, and biological sample examination.

Automatically generated - may contain errors

Lab products found in correlation

Digital sight ds 5m l1 camera, by Nikon (2 mentions) Shandon cytospin 3, by Thermo Fisher Scientific (1 mentions) Nis elements software, by Nikon (1 mentions) Nis elements d 3, by Nikon (1 mentions) Bx60 microscope, by Olympus (1 mentions) Endo porter delivery system, by Gene Tools (1 mentions) X gal staining, by Merck Group (1 mentions) Plan fluor 40x 0, by Nikon (1 mentions) Digital sight ds fi1 camera, by Nikon (1 mentions) Anti iba1, by Fujifilm (1 mentions) Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions) Super block, by ScyTek Laboratories (1 mentions) Anti cleaved casp3, by Cell Signaling Technology (1 mentions) Em uc6 ultramicrotome, by Leica (1 mentions) Bx 40, by Leica (1 mentions) Spss statistics version 23, by IBM (1 mentions) Hematoxylin and eosin, by Leica (1 mentions) Ds fi1, by Nikon (1 mentions) Nis elements d image analysis software, by Nikon (1 mentions) Vectastain abc reagent, by Vector Laboratories (1 mentions)

Spelling variants of this product

Eclipse 50i (459 citations) Eclipse 50i microscope (228 citations) Eclipse microscope (191 citations) Eclipse light microscope (21 citations) Nikon 50i microscope (18 citations) Nikon 50i light microscope (8 citations) ECLIPSE 50i light microscope system (2 citations)

24 protocols using eclipse 50i light microscope

Quantifying Leishmania Infection in Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDM were infected as described above. The L. m.-infected BMDM and control BMDM were incubated for time points ranging from 0.5 to 48 h. After incubation, 2 × 10⁵ BMDM were transferred to Cytospin tubes (Thermo Scientific). BMDM were attached to object slides by centrifugation at 1500 rpm for 5 min with a Shandon Cytospin3 (Thermo Scientific). Afterwards, the slides were fixed and stained with a Diff-Quik kit (Medion Diagnostics, 130832) according to the manufacturer’s protocol. The slides were analyzed with an Eclipse 50i light microscope (Nikon) using NIS Elements software version 3.22.11 (Nikon). To calculate the average infection rates, the number of intracellular parasites per individual macrophage for each analyzed sample was determined. For each sample, 50 individual macrophages were analyzed.
During differentiation of L. m. promastigotes (0 h p.i.) to amastigotes (24 h p.i.), the nucleus-kinetoplast distance shortened significantly from approximately 4 μm to 1.8 μm. The average nucleus-kinetoplast distances were determined by measuring the distances of 50 individual intracellular parasites with an Eclipse 50i light microscope (Nikon) and NIS Elements software version 3.22.11 (Nikon). Statistical significance for the average infection rates, or the average nucleus-kinetoplast distances, was tested with a t-test in SPSS software version 20.0.0 (IBM).

Frank B., Marcu A., de Oliveira Almeida Petersen A.L., Weber H., Stigloher C., Mottram J.C., Scholz C.J, & Schurigt U. (2015). Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210. Parasites & Vectors, 8, 404.

+ Open protocol

+ Expand

Histological Analysis of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histological analyses were performed on NBPs, NPPs, DBPs and DPPs following snap freezing in liquid nitrogen. Briefly, samples were fixed in 4% (w/v) paraformaldehyde (PFA) for 20 min at room temperature in darkness, maintained in 20% sucrose in PBS overnight at 4 °C and embedded in a 1:1 solution of 20% (w/v) sucrose and Optimum Cutting Temperature (OCT) compound (Tissue-Tek, Alphen Aan den Rijn, Netherlands). Tissue cryosections (7 µm) were stained with hematoxylin/eosin (H&E), Mallory trichrome (MT), Alcian Blue (AB), and Weigert-Van Gieson (VG), all supplied by Bioptica (Milan, Italy). Images were acquired with a Nikon Eclipse 50i light microscope equipped with a NIS-Elements D3.2 Software (Nikon Corporation, Shinagawa, Tokyo, Japan).

Zouhair S., Dal Sasso E., Tuladhar S.R., Fidalgo C., Vedovelli L., Filippi A., Borile G., Bagno A., Marchesan M., De Rossi G., Gregori D., Wolkers W.F., Romanato F., Korossis S., Gerosa G, & Iop L. (2020). A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications. Biomolecules, 10(3), 371.

+ Open protocol

+ Expand

Alizarin Red Staining of Corneal Endothelium

Check if the same lab product or an alternative is used in the 5 most similar protocols

A solution of Alizarin red (0.1%) was diluted with 0.9% saline. The deep-iodine coloured solution was filtered (2-μm filter) to remove any undissolved sediment. The pH of the solution was then adjusted to 4.2 with diluted ammonium hydroxide (0.1% solution in normal saline). Excised corneas were placed in plastic vessels with the endothelial side up, immersed in an Alizarin red solution for 90 seconds, and then rinsed three times with saline to wash out the staining reagent. After the staining procedure, corneas were immersed in a 4% paraformaldehyde solution for 10 minutes at room temperature (RT) and then again rinsed three times with saline. Four radial incisions were made across each cornea to flat-mount it endothelial side up and examined under a Nikon Eclipse 50i light microscope (Nikon, Japan). The central cornea was regarded as the region in which the tissue diameter was less than 3mm, whereas the remaining regions were referred to as the peripheral cornea. Three images each were separately captured from the central and peripheral regions of the corneal endothelium in the 4 different quadrants. The ultimate ECDs for the central and peripheral areas were calculated by averaging the 12 ECDs.

Li X., Zhang Z., Ye L., Meng J., Zhao Z., Liu Z, & Hu J. (2017). Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells. Scientific Reports, 7, 6951.

+ Open protocol

+ Expand

Histopathological Evaluation of Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

For hematoxylin and eosin (H&E) general histopathology evaluation, lungs were rapidly isolated, and fixed in 4% neutral-buffered PFA at room temperature (RT) for 2 weeks, followed by routine processing for paraffin embedding. Coronal, serial sections, 4–5 µm thick, were performed and selected sections were stained with H&E for light microscopy examination. Images were acquired using Nikon Eclipse 50i Light Microscope (Nikon, Tokyo, Japan) or Olympus microscope (BX60)

Yahalom-Ronen Y., Tamir H., Melamed S., Politi B., Shifman O., Achdout H., Vitner E.B., Israeli O., Milrot E., Stein D., Cohen-Gihon I., Lazar S., Gutman H., Glinert I., Cherry L., Vagima Y., Lazar S., Weiss S., Ben-Shmuel A., Avraham R., Puni R., Lupu E., Bar-David E., Sittner A., Erez N., Zichel R., Mamroud E., Mazor O., Levy H., Laskar O., Yitzhaki S., Shapira S.C., Zvi A., Beth-Din A., Paran N, & Israely T. (2020). A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge. Nature Communications, 11, 6402.

+ Open protocol

+ Expand

Quantification of Inflammatory Cells in Murine Ears

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the end of the experimental period, the ears of 6 mice from each group were removed, fixed in 10% neutral phosphate-buffered formaldehyde, and cut into 3 segments ~2 mm in width. After the ear segments were embedded in paraffin, 2 sections ~3 µm in thickness were obtained from each one. One of the sections was stained with hematoxylin and eosin, while the other one was stained with toluidine blue. Three points on each segment were arbitrarily selected for indicating the apical, central, and basal regions, and the number of inflammatory cells in the subcutaneous tissue of each of the 9 areas (3 ear segments × 3 regions) was counted using a Nikon ECLIPSE 50i light microscope (Nikon Co., Tokyo, Japan). The evaluated area was defined by a perpendicular line extending from the edge of the cartilage of the external ear by a length of 100 µm (for eosinophil count) or 400 µm (for mast cell count) to the epidermal layer. Mast cell degranulation was scored as previously described 17 (link): non-degranulated (0%), mildly degranulated (0-50%), and severely degranulated (>50%).

Sadakane K, & Ichinose T. (2015). Effect of the Hand Antiseptic Agents Benzalkonium Chloride, Povidone-Iodine, Ethanol, and Chlorhexidine Gluconate on Atopic Dermatitis in NC/Nga Mice. International Journal of Medical Sciences, 12(2), 116-125.

+ Open protocol

+ Expand

Lung Histopathology Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For hematoxylin and eosin (H&E) general histopathology evaluation, lungs were rapidly isolated, and fixed in 4% neutral-buffered PFA at RT for 7 days followed by routine processing for paraffin embedding. Coronal, serial sections, 4–5 µm thick, were performed and selected sections were stained with H&E for light microscopy examination. Images were acquired using a Nikon Eclipse 50i Light Microscope (Nikon, Tokyo, Japan) or Olympus BX60 microscope (Shinjuku, Tokyo, Japan).

Li H., Zhang Y., Li D., Deng Y.Q., Xu H., Zhao C., Liu J., Wen D., Zhao J., Li Y., Wu Y., Liu S., Liu J., Hao J., Yuan F., Duo S., Qin C.F, & Zheng A. (2021). Enhanced protective immunity against SARS-CoV-2 elicited by a VSV vector expressing a chimeric spike protein. Signal Transduction and Targeted Therapy, 6, 389.

+ Open protocol

+ Expand

Kidney Organ Culture for VEGF-LacZ Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney organ culture and tissue delivery of target site protector oligos were carried out as reported previously with slight modifications10 (link). Briefly, kidneys from VEGF-LacZ mice were collected under sterile conditions. Kidney capsules were removed and kidney cortices dissected and cut into small pieces (∼1 mm). Kidney cortex pieces were cultured using a roller bottle incubator (model 1,000; Robbins Scientific, Sunnyvale, CA, USA) at 37 °C, 5% CO₂, 20% O₂, 75% N₂ for 72 h in Dulbecco's modified Eagle's medium, containing 10% fetal bovine serum, 1 × anti-anti (Gibco-LifeTech), under either normal glucose (5 mM D-glucose) or high glucose conditions (25 mM D-glucose). Overall, 10 μM of a VEGF target site protector or NT target site protector were delivered into the cultured kidney pieces using 4 mM of the Endo-Porter delivery system (Gene Tools). After 72 h, kidney pieces were fixed in 2% paraformaldehyde and 0.2% glutaraldehyde. X-gal staining (Millipore) was performed at 37 °C in 0.02% glutaraldehyde, 5 mM K₃Fe(CN)₆, 5 mM K₄Fe(CN)6, and 2 mM MgCl₂ as described previously10 (link). Kidney pieces were then post fixed in 4% paraformaldehyde followed by paraffin embedding. Paraffin sections were dewaxed and mounted onto glass slides for image acquisition. All images were obtained on Nikon Eclipse 50i Light microscope (Nikon Inc, Tokyo, Japan).

Badal S.S., Wang Y., Long J., Corcoran D.L., Chang B.H., Truong L.D., Kanwar Y.S., Overbeek P.A, & Danesh F.R. (2016). miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Nature Communications, 7, 12076.

+ Open protocol

+ Expand

Immunostaining for IMP3, p53, and Ki-67

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostaining for IMP3 showed a cytoplasmic or membranous pattern (Fig. 1). Nuclear expression for p53 and Ki-67 were accepted as positive. Two pathologists, blinded to clinicopathologic variables and clinical outcome, evaluated the intensity and extent of the immunostaining. IMP3 and p53 expression levels were semi-quantitatively assessed and classified into negative and positive groups. The negative group was defined as cases in which the number of cells stained was less than 5%, while the positive group included cases in which the number of cells stained exceeded 5%. The 5% cut-off appeared to be optimal for stratifying expression. Quantification of Ki-67 PI was performed by assessing the percentage of stained tumor cells using a Nikon eclipse 50i light microscope (Nikon, Tokyo, Japan) with a 40× objective lens (Plan Fluor 40 X /0.75, Nikon). The percentage of stained cells was examined in the maximally stained area with 5 consecutive high power fields (HPF) (field area of one HPF, 0.307 mm²). The average percentage of stained cells was recorded after repeating the process three times. Ki-67 status was then classified as a low (less than 10% tumor cells) or a high (more than 10%) PI group.

Park J.Y., Choe M., Kang Y, & Lee S.S. (2014). IMP3, a Promising Prognostic Marker in Clear Cell Renal Cell Carcinoma. Korean Journal of Pathology, 48(2), 108-116.

+ Open protocol

+ Expand

Recombinant S100A8 Protein in Rabbit Wound Healing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal experiments in this study were approved by the Northwestern University Animal Care and Use Committee. Female New Zealand white rabbits were purchased from Covance. Six excisional wounds (7 mm full thickness) were generated on the ventral surface of each rabbit ear. A semiocclusive dressing, Tegaderm, was placed onto the wounded ear to prevent desiccation. When the wounds were fully reepithelialized, approximately 14 days after wounding, recombinant rabbit S100A8 protein was injected into the wounds at postoperative days (PODs) 15, 19, and 23 (10 mg per wound, each time). Saline was injected into the wounds in the contralateral ear as a control. The rabbit wounds were harvested at POD 28, fixed in formalin, embedded in paraffin, and divided into sections (4 mm thick). The histological slides were stained for hematoxylin and eosin dyes and analyzed using a Nikon Eclipse 50i light microscope (Nikon Instruments Inc., Melville, NY). The scar elevation index (SEI) was calculated. 30, 31

Zhong A., Xu W., Zhao J., Xie P., Jia S., Sun J., Galiano R.D., Mustoe T.A, & Hong S.J. (2016). S100A8 and S100A9 Are Induced by Decreased Hydration in the Epidermis and Promote Fibroblast Activation and Fibrosis in the Dermis. The American journal of pathology, 186(1).

+ Open protocol

+ Expand

Intestinal Morphometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Segments of the small intestine (jejunum, JI and ileum) were processed and embedded in paraffin wax and stained with haematoxylin and eosin (Figure 1). Overall severity score of intestinal damage was assessed using a semi-quantitative analysis based on 11 parameters as described by Howarth et al. 34 . A score from 0 (unaffected) to 3 (severe) was recorded to provide a maximum damage severity score of 33 for each intestinal region. Villus height and crypt depth measurements were determined (40 villi and crypts/ rat) using a Nikon Eclipse 50i light microscope (Nikon Corporation, Japan) with ProgRes C5 laser optik digital camera (Jenoptik, Germany) and Image-Pro Plus Software Package Version 5.1 (Media Cybernetics, Maryland, USA).

Mashtoub S., Lampton L.S., Eden G.L., Cheah K.Y., Lymn K.A., Bajic J.E, & Howarth G.S. (2016). Emu Oil Combined with Lyprinol™ Reduces Small Intestinal Damage in a Rat Model of Chemotherapy-Induced Mucositis. Nutrition and cancer, 68(7).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!