Tissues were fixed in 10% formalin and processed using standard procedures. In situ hybridization was performed as previously described (Herriges et al., 2014 (link); Morrisey et al., 1996 (link); Wang et al., 2013 (link)). Immunohistochemistry was performed using the following antibodies: goat anti-Scgb1a1 (Santa Cruz, 1:20), rabbit anti-SP-C (Chemicon, 1:500), rabbit anti-Nkx2.1 (Santa Cruz, 1:50), rabbit anti-Sox9 (Santa Cruz, 1:100), rabbit anti-Sox2 (Seven Hills Bioreagents, 1:500), mouse anti-β-tubulin IV (BioGenex, USA; 1:20), rabbit anti-e-cadherin (Cell Signaling, 1:100), Anti-Mouse CD31 (PECAM-1) (HistoBioTec, 1:20). The Foxp1 (1:200), Foxp2 (1:200) and Foxp4 (1:200) polyclonal antibodies have been previously described (Lu et al., 2002 (link)).
Rabbit anti sp c
Manufactured by Merck Group
Sourced in United States
The Rabbit anti-SP-C is a primary antibody that recognizes the surfactant protein C (SP-C) antigen. SP-C is a small hydrophobic protein found in the lamellar bodies of type II alveolar cells in the lung. The Rabbit anti-SP-C can be used in various applications, such as immunohistochemistry and western blotting, to detect and analyze the expression of SP-C in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti nkx2, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti sox9, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti e cadherin, by Cell Signaling Technology (1 mentions)
Goat anti scgb1a1, by Santa Cruz Biotechnology (1 mentions)
Vectashield mounting medium containing dapi, by Vector Laboratories (1 mentions)
Goat anti cc10, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti cdh1, by Cell Signaling Technology (1 mentions)
Mouse anti cdkn1a, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rabbit anti sp c
1
Immunohistochemical Analysis of Lung Development
2
Immunohistochemistry and in-situ Hybridization
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Tissues were fixed in 4% paraformaldehyde, embedded in paraffin wax, and sectioned at 5-μm intervals. Immunohistochemistry was performed using the following antibodies: mouse anti-phopho-histone3 (1:200; Cell Signaling Technology), goat anti-CC10 (1:20; Santa Cruz Biotechnology), rabbit anti-SP-C (1:500; Chemicon), rabbit anti-Nkx2.1 (1:50; Santa Cruz Biotechnology), mouse anti-Cdkn1a (1:100; Santa Cruz Biotechnology), rabbit anti-Sox9 (1:100; Santa Cruz Biotechnology), rabbit anti-Rbl2/p130 (1:50; Abcam), rabbit anti-Par3 (1:100; Upstate Biotechnology), rabbit anti-Sox2 (1:500; Seven Hills Bioreagents), and rabbit anti-Cdh1 (1:100; Cell Signaling). Slides were mounted with Vectashield mounting medium containing DAPI (Vector Laboratories). ISH was performed as described (Zhang et al. 2008 (link)). Probes were amplified from each lncRNA using the primers listed in Supplemental Table 8.
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