Discovery dst

All patients underwent clinically indicated PET MPI using 13N-ammonia acquired at rest and during pharmacological stress (adenosine infused at 0.14 mg⋅kg⁻¹⋅min⁻¹ over 6 minutes or single bolus injection of 400 mcg of regadenosone) according to clinical routine. All data were acquired in list-mode on a PET/CT scanner (Discovery DST, Discovery MI or Discovery VCT, all GE Healthcare, Waukesha, WI, USA) as previously reported.^{16 (link)} In brief, a body mass index-adapted dose of 13N-ammonia (i.e., 400-1200 Megabecquerels) was injected and the datasets were reconstructed using ordered subset expectation maximum (OSEM, VUE Point HD or VUE Point FX with 2 iterations and 16 subsets), and a 5 mm Hanning filter and standard decay, scatter and sensitivity corrections (voxel size 2.34, 2.34, 2.80-3.27) were applied. Low-dose unenhanced computed tomography was used for attenuation correction. Dynamic datasets were reconstructed from the first 7 minutes of acquisition and consisted of 9 frames of 10 seconds duration, 6 frames of 15 seconds, 3 frames of 20 seconds, 2 frames of 30 seconds and 1 frame of 120 seconds. MBF at rest (corrected for the rate-pressure-product) and during stress and MFR was calculated using commercially available software (QPET 2017.7 Cedars-Sinai Medical Center, Los Angeles, CA, USA). Static datasets were reconstructed from the following 10 minutes of the acquisition.