Anti pax5

Manufactured by Abcam

Sourced in United States

Anti-PAX5 is a primary antibody that recognizes the PAX5 protein. PAX5 is a transcription factor that plays a crucial role in B-cell development and differentiation. This antibody can be used in various applications, such as western blotting, immunohistochemistry, and flow cytometry, to detect and analyze the expression of PAX5 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd8, by Thermo Fisher Scientific (2 mentions) Anti ha, by Cell Signaling Technology (2 mentions) Anti cd4, by Thermo Fisher Scientific (2 mentions) Meca79, by Thermo Fisher Scientific (2 mentions) Anti cd3, by Abcam (2 mentions) Anti sox2, by Abcam (1 mentions) Anti nanog, by Active Motif (1 mentions) Anti klf4, by Abcam (1 mentions) Anti oct4, by Abcam (1 mentions) Anti ctcf, by Merck Group (1 mentions) Anti brd4, by Fortis Life Sciences (1 mentions) Anti cebpb, by Abcam (1 mentions) Anti ets1, by Active Motif (1 mentions) Anti ikaros, by Active Motif (1 mentions) Anti p300, by Active Motif (1 mentions) Histone h3 23 34 peptide, by AnaSpec (1 mentions) Kaarksapatgg, by AnaSpec (1 mentions) Histone h3k27ac 23 34 peptide, by AnaSpec (1 mentions) Kaar k ac sapatgg, by AnaSpec (1 mentions) H3k27ac, by Active Motif (1 mentions)

Spelling variants of this product

Anti-PAX5 antibody (2 citations)

6 protocols using anti pax5

Histone Modifications and Chromatin Interactions

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal to H1, HMGN1, HMGN2, and H3 were from our laboratory, anti H3K27ac (Abcam#ab4729), anti H3K27me3 (Abcam#ab6002), monoclonal anti H1(Milipore-Sigma #05-457), anti CEBPB (Abcam#ab32358), Anti-Brd3 (Active Motif #61489), Anti-Brd4 (Bethyl Laboratories #A301-985A100), Anti-CEBPB (Abcam #ab32358), Anti-CTCF (EMD Millipore #07-729), Anti-Ets1 (Active Motif #39580), Anti-Ikaros (Active Motif #39355), Anti-Irf8 (Bethyl Laboratories #A304-027A), Anti-Klf4 (Abcam #106629), Anti-Nanog (Active Motif #61419), Anti-Oct4 (Abcam #ab19857), Anti-p300 (Active Motif #61401), Anti-Pax5 (Abcam #183575), Anti-Sox2 (Abcam #97959).
The following recombinant mononucleosomes were purchased from Active Motif: unmodified (#81070); H3K27me3 modified (#81834), H3K27ac modified (#81077).
Wild type and HMGN DKO mouse embryonic fibroblasts, embryonic stem cell lines^{55 (link)} and resting B cells^{56 (link)} were as previously described. Peptides Histone H3 (23–34) peptide, KAARKSAPATGG and Histone H3K27ac (23–34) peptide, KAAR - K(Ac)—SAPATGG were from AnaSpec, Inc.

Zhang S., Postnikov Y., Lobanov A., Furusawa T., Deng T, & Bustin M. (2022). H3K27ac nucleosomes facilitate HMGN localization at regulatory sites to modulate chromatin binding of transcription factors. Communications Biology, 5, 159.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Lymph Nodes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymph nodes were fixed in 10% neutral-buffered formalin and embedded in paraffin. Tissue sections were typically 4-μm thick and were examined after staining with hematoxylin and eosin (H&E). For immunohistochemistry, the sections were first boiled in a citrate-based solution to retrieve antigens and subsequently quenched in 3% hydrogen peroxide. The antibodies used included anti-FOXP3 (Cat. MAB8214, R&D systems, Minneapolis, MN), anti-PD-1 (Cat. AF1021, R&D systems), anti-CD3 (Cat. ab5690, Abcam, Cambridge, MA), anti-PAX5 (Cat. sc-1974, Santa Cruz Biotechnology, Dallas, TX), anti-CD4 (Cat. 14–0042, eBioscience, San Diego, CA), anti-CD8 (Cat. 14–0081, eBioscience), anti-CD21 (Cat. Ab75985, Abcam), MECA79 (Cat. 53–6036-80, eBioscience) and anti-HA (Cat. 3724, Cell Signaling Technology) antibodies. After biotinylated secondary antibody treatment, visualization was carried out with Vectastain ABC-HRP kit (Vector Laboratories, Burlingame, CA) or ABC-AP kit (Vector Laboratories) in combination with DAB or with VectorRed (Vector Laboratories) respectively.

Lee G.J., Jun Y., Jeon Y.K., Lee D., Lee S, & Kim J. (2022). Mice transgenic for human CTLA4-CD28 fusion gene show proliferation and transformation of ATLL-like and AITL-like T cells. Oncoimmunology, 11(1), 2015170.

+ Open protocol

+ Expand

Multimodal Immunohistochemical Analysis of Lymph Nodes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymph nodes were fixed in 10% neutral buffered formalin and embedded in paraffin. Sections were typically four μm thick and stained with hematoxylin and eosin (H&E). For immunohistochemistry, sections were boiled in citrate-based solution to retrieve antigens and subsequently quenched in 3% hydrogen peroxide. Antibodies used included anti-CD21 (Cat. ab75985, Abcam, Cambridge, MA, USA), MECA79 (Cat. 53-6036-80, eBioscience, San Diego, CA, USA), anti-PD-1 (Cat. AF1021, R&D systems, Minneapolis, MN, USA), anti-CD3 (Cat. ab5690, Abcam), anti-PAX5 (Cat. sc-1974, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD4 (Cat. 14–0042, eBioscience), anti-CD8 (Cat. 14–0081, eBioscience), anti-CD138 (Cat. AF3190, R&D Systems) and anti-HA (Cat. 3724, Cell Signaling Technology). Horseradish peroxidase-coupled secondary antibodies (Vector Laboratories, Burlingame, CA, USA) were used in combination with DAB for visualization. For immunofluorescence staining, sections were stained with Alexa Fluor 488 conjugated anti-GL7 (Cat. 144611, BioLegend, San Diego, CA, USA) and anti-CD4 (Cat. 14–0042, eBioscience). Secondary antibody for anti-CD4 was goat anti-Rat IgG (H + L) Alexa Fluor 594 (Cat. A11007, Invitrogen, Carlsbad, CA, USA).

Lee G.J., Jun Y., Yoo H.Y., Jeon Y.K., Lee D., Lee S, & Kim J. (2020). Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation. Oncoimmunology, 9(1), 1746553.

+ Open protocol

+ Expand

Histological Analysis of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

All tissues were fixed in formalin, embedded in paraffin, sectioned at 4 μm, mounted on slides (Superfrost Plus; Thermo Fisher Scientific), and dried at 60°C for 20 min before dewaxing and staining with hematoxylin and eosin (H&E) using standard methods. For immunohistochemical staining, the primary antibodies used in this study included anti-B220 (BD Biosciences) and anti-PAX5 (Abcam). Sections underwent antigen retrieval in a prediluted Cell Conditioning Solution (CC1) (Ventana Medical Systems) for 32 min, and the OmniMap anti-Rabbit HRP kit (Ventana Medical Systems) and ChromoMap DAB (Ventana Medical Systems) were used for detection. Sections were examined by a pathologist blinded to the experimental group assignments.

Smith K.H., Budhraja A., Lynch J., Roberts K., Panetta J.C., Connelly J.P., Turnis M.E., Pruett-Miller S.M., Schuetz J.D., Mullighan C.G, & Opferman J.T. (2020). The Heme-regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics. Molecular cancer research : MCR, 19(4), 636-650.

+ Open protocol

+ Expand

Immunoblotting Assay for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting, 1.5 × 10⁶ cells per sample were harvested and lysed in 50 μl modified RIPA buffer (50 mM TrisHCl, pH 7.4, 1% NP-40, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA (pH 8), 1 mM sodium orthovanadate, 1 mM NaF and protease inhibitor cocktail (Sigma-Aldrich)). Lysates were separated on 10% SDS-polyacrylamide gels and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% dry milk in PBT (PBS, 0.1% Tween-20) for 1 h at room temperature with constant agitation. Primary antibodies were diluted in PBT supplemented with 4% BSA fraction V (BIOMOL Research Laboratories). Secondary antibodies were diluted in blocking solution. Immunoreactive antibodies used were anti-Pax5 (Abcam), anti-FoxO1 (Cell Signaling Technologies), and anti-Actin (I-19, Santa Cruz Biotechnology).

Abdelrasoul H., Werner M., Setz C.S., Okkenhaug K, & Jumaa H. (2018). PI3K induces B-cell development and regulates B cell identity. Scientific Reports, 8, 1327.

+ Open protocol

+ Expand

ChIP Profiling of PAX5 Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIP was performed on 697 parental, 697-PAX5 P80R/Fs, 697-PAX5 WT/Fs, and 697-PAX5 WT/WT using ChIP-IT High Sensitivity Kit (Active Motif, 53040) according to the manufacturer’s protocol. A total of 2 × 10⁶ cells were fixed with complete cell fixation solution for 15 min and then stopped by stop solution for 5 min, followed by sonication. Both buffers were provided by the ChIP-IT High Sensitivity Kit. The following antibodies were used for ChIP assays: anti-PAX5 (Abcam, ab15164), anti-H3K27Ac [Cell Signaling Technology (CST), 8173S], and anti-H3K4me3 (CST, 9751). Normal rabbit immunoglobulin G (CST, 2729) was used for control ChIP. Primers for ChIP-qPCR analysis are shown in table S5.

Li Y., Moriyama T., Yoshimura S., Zhao X., Li Z., Yang X., Paietta E., Litzow M.R., Konopleva M., Yu J., Inaba H., Ribeiro R.C., Pui C.H, & Yang J.J. (2022). PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia. Science Advances, 8(50), eadd6403.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!