Anti nrf2 ab89443

Anti-Nrf2 (ab89443) purchased from Abcam (Cambridge, UK). Anti-β-actin (20536-1-AP), anti-BAD (10435-1-AP), p-CREB (12208-1-AP), LMNA (10298-1-AP) antibodies were obtained from Proteintech Group Co., Ltd. (Wuhan, PRC). Anti-AKT1(K101311P), anti-p-AKT1(K006214P), PI3K(K106692P), p-PI3K(K006379P) antibodies were obtained from Solarbio Science & Technology(Beijing, PRC). Anti-BCL-2(AF6139), Cleaved-Caspase 3(AF7022), Caspase 3(AF6311), Cleaved-Caspase 9(AF5240), Caspase 9(AF6348) antibodies were obtained from, Affinity Biosciences (USA). Vincristine (VCR) were purchased from Topscience (Shanghai, PRC). Daunorubicin (DNR) and brusatol (an Nrf2 inhibition) were purchased from MCE (NJ, USA). The chemical inhibitor MK-2206 (MCE, NJ, USA) of the AKT (39 (link)). Fetal bovine serum and RPMI 1640 medium were obtained from Gibco (Carlsbad, CA, USA). Nuclear Protein Extraction Kit were obtained from Solarbio Science & Technology (Beijing, PRC).

Anti-GPX4 (ab125066), anti-NRF2 (ab89443), and anti-SLC7A11 (ab37185) were purchased from Abcam, Shanghai, China, and anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#2118) was obtained from Cell Signaling Technology (CST, Shanghai, China). Anti-rabbit IgG, anti-mouse IgG, and horseradish peroxidase (HRP)-linked antibody (#7074, #7076) were also purchased from CST. Primer sequences were customized from Tsingke Biotechnology Co., Ltd (Shanghai, China).