Zbtb46 dtr

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Zbtb46-DTR is a transgenic mouse line that expresses the diphtheria toxin receptor (DTR) under the control of the Zbtb46 gene promoter. The Zbtb46 gene is a marker for a specific subset of dendritic cells. The expression of the DTR allows for the selective depletion of these dendritic cells upon administration of diphtheria toxin.

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Zbtb46-DTr mice (2 citations)

7 protocols using zbtb46 dtr

Murine Plasmodium Infection Model

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Female mice of C57BL/6 (WT), Aim2^−/−, Casp1^−/−, Myd88^−/−, Nlrp3^−/−, Il1r1^−/− and Zbtb46-DTR mice were purchased from The Jackson Laboratory. Traf3^flox/flox mice were kindly gifted from Dr. Shao-Cong Sun (University of Texas, MD Anderson Cancer Center) and cross with CD11c-cre (The Jackson Laboratory) to generate Traf3^f/f CD11c-cre mice, Irf3^−/−:Irf7^−/− mice were from Dr. Kate Fitzgerald (University of Massachusetts Medical School) and Dr. Tadatsugo Taniguchi (The University of Tokyo), and crossed with C57BL/6 mice to get Irf3^−/− mice. For plasmodium infection, 0.5 × 10⁶ iRBCs (otherwise, indicated specifically in the figure legend) suspended in 200 µl PBS from the donor mice were intraperitoneally injected into experimental mice. All mouse-related procedures were performed according to experimental protocols approved by the Animal Care and Welfare Committee at Houston Methodist Research Institute and in accordance with NIH-approved animal study protocol LMVR-11E.

Yu X., Du Y., Cai C., Cai B., Zhu M., Xing C., Tan P., Lin M., Wu J., Li J., Wang M., Wang H.Y., Su X.Z, & Wang R.F. (2018). Inflammasome activation negatively regulates MyD88-IRF7 type I IFN signaling and anti-malaria immunity. Nature Communications, 9, 4964.

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Mouse Genetic Knockout Models

Cited 2 times

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C57BL/6J and μMT, Il1r1^−/−, and Zbtb46-DTR mice were purchased from the Jackson Laboratories. Trif^−/− mice were originally provided by S. Akira and obtained from Ruslan Medzhitov at Yale University. Casp1^−/−Casp11^129mt/129mt were provided by Richard Flavell at Yale University. Ifnar1^−/− and Irf3^−/− were provided by Carolina Lopez at the University of Pennsylvania. Trif^−/−, Irf3^−/− or Casp1^−/−Casp11^129mt/129mt mice were interbred with μMT mice to generate μMTxTrif^−/−, μMTx Irf3^−/− and μMTx Casp1^−/−Casp11^129mt/129mt mice. CCR2-CFP-DTR mice were provided by Eric Pamer at Memorial Sloan Kettering (Hohl et al., 2009 (link)), and CX3CR1-STOP-DTR and CD11c-CRE mice were provided by Iliyan Iliev at Weill Cornell Medicine (Diehl et al., 2013 (link)). We used 6-10 week-old mice (male and female) for all experiments. All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at the Icahn School of Medicine at Mount Sinai as well as Weill Cornell Medicine (New York, NY, USA), and carried out in accordance with the ‘Guide for the Care and Use of Laboratory Animals’ (NIH publication 86-23, revised 1985).

Barbet G., Sander L.E., Geswell M., Leonardi I., Cerutti A., Iliev I, & Blander J.M. (2018). Sensing microbial viability through bacterial RNA augments T follicular helper cell and antibody responses. Immunity, 48(3), 584-598.e5.

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Genetically Modified Mouse Models

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C57BL/6, Il17a^GFP, Zbtb46^GFP, Zbtb46^DTR, and OT-II mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Tmem173^−/− (Sting^−/−) mice on C57BL/6 background were generated using CRISPR/Cas9 technique in Nanjing Biomedical Research Institute of Nanjing University. All mice were maintained in H. hepaticus- and Pasteurella-free or specific pathogen-free environment at MARC (Model Animal Research Center of Nanjing University). All animal experiments in this study were undertaken when mice were 6–10 weeks old with protocols approved by the Institutional Subcommittee on Research Animal Care at Nanjing University.

Liu S., Xia Q., Wu X., Sun F., Hu Q., Wu J., Wang M., Rao Q, & Guan W. (2018). Stimulator of Interferon Genes in Classical Dendritic Cells Controls Mucosal Th17 Responses to Cyclic Dinucleotides for Host Defenses Against Microbial Infections in Gut. Frontiers in Immunology, 9, 1085.

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Generating Diverse Murine Genotypes

Cited 1 time

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The following mice were purchased from Jackson Laboratories: C57BL/6, Tcra^−/−, Zbtb46-DTR^+/+, MHCII deficient (homozygous H2^dlAb1-Ea), Il6^−/−, Il12p40^−/−, Zbtb46-cre^+/+, Il6ra^flox/flox, Irf5^flox/flox, Adam17^flox/flox, Batf3^−/−, Tlr4^−/−, Tlr7^−/− and Ifnar1^−/−. The cDC-Il6ra^−/−, cDC-Irf5^−/− and cDC-Adam17^−/− genotypes were generated by crossing the Zbtb46-cre^+/+ with Il6ra^flox/flox, Irf5^flox/flox, or Adam17^flox/flox, respectively. The Irf5^−/− animals were as described (Zhao et al., 2019 (link)). All genotypes were confirmed by PCR, according to the instructions provided by Jackson Laboratories. Animal experiments were performed on mice aged 6–12 weeks.

Yousif A.S., Ronsard L., Shah P., Omatsu T., Sangesland M., Bracamonte Moreno T., Lam E.C., Vrbanac V.D., Balazs A.B., Reinecker H.C, & Lingwood D. (2020). The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells. Immunity, 54(2), 235-246.e5.

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Genetically Modified Mouse Models

Cited 2 times

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C57BL/6 WT, CXCR3-GFP (CIBER) and Zbtb46^DTR mice were obtained from Jackson Laboratory. All mice, including REX3-Tg (Groom et al., 2012 (link)), Cxcr3^−/− (Hancock et al., 2000 (link)), Cxcl9^−/− (Park et al., 2002 (link)), and Cxcl10^−/− (Dufour et al., 2002 (link)) were in the C57BL/6 background and were housed under specific pathogen-free conditions. All procedures were approved by the Massachusetts General Hospital Subcommittee on Research and Animal Care.

Chow M.T., Ozga A.J., Servis R.L., Frederick D.T., Lo J.A., Fisher D.E., Freeman G.J., Boland G.M, & Luster A.D. (2019). Intratumoral activity of the CXCR3 chemokine system is required for the efficacy of anti- PD-1 therapy. Immunity, 50(6), 1498-1512.e5.

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In Vivo Murine Immunological Protocols

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All animals were bred and housed under specific pathogen free conditions at the Massachusetts General Hospital. Experiments were approved by the MGH Institutional Animal Care and Use Committee (IACUC) and were performed in accordance with MGH IACUC regulations. The following mouse strains were used in this study : Female C57BL6/J mice (8 – 12 week old) were purchased from Jackson Laboratories (Bar Harbor, ME). REAT (IFN- -IRES-eYFP Cat #017581), IL-12p40-IRES-eYFP (Cat #006412), CD11c-cre (Cat #007567), Ifngr1fl/fl (Cat #025394), and Zbtb46-DTR (Cat# 025394) were obtained from Jackson Laboratories.

Garris C.S., Arlauckas S.P., Kohler R.H., Trefny M.P., Garren S., Piot C., Engblom C., Pfirschke C., Siwicki M., Gungabeesoon J., Freeman G.J., Warren S.E., Ong S., Browning E., Twitty C.G., Pierce R.H., Le M.H., Algazi A.P., Daud A.I., Pai S.I., Zippelius A., Weissleder R, & Pittet M.J. (2018). Successful anti-PD-1 cancer immunotherapy requires T cell-dendritic cell crosstalk involving the cytokines IFN-γ and IL-12. Immunity, 49(6), 1148-1161.e7.

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Murine Cancer Models and Characterization

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Experiments utilized 6–8 week old C57BL/6 (#000664), B6.SJL (#002014) and Zbtb46-DTR (#019506) mice that were obtained from The Jackson Laboratories. 2C TCR transgenic mice were kindly provided by Dr. Thomas Gajewski at the University of Chicago. Survival experiments were performed with 5–8 mice per experimental group, and mechanistic experiments with 4–6 mice per group. Animal protocols were approved by the Earle A. Chiles Research Institute (EACRI) Institutional Animal Care and Use Committee (Animal Welfare Assurance No. A3913–01). The Panc02-SIY pancreatic adenocarcinoma line expressing the model antigen SIY was kindly provided by Dr. Ralph Weichselbaum at the University of Chicago. MC38 colorectal carcinoma line was obtained from Dr. Kristina Young at EACRI. Moc1 and Moc2 oral squamous cell carcinoma lines were kindly provided by Dr. Ravindra Uppaluri at the Dana Faber Cancer Institute. Panc02-SIY, Moc1 and Moc2 cell lines were grown in complete RPMI containing 10% heat inactivated fetal bovine serum (FBS), 100U/mL penicillin, 100μg/mL streptomycin. MC38 cell lines were grown in DMEM containing 10% heat inactivated FBS, 100U/mL penicillin, 100μg/mL streptomycin. Pathogen and mycoplasma contamination testing were performed on all cell lines within the past 6 months using the IMPACT II Mouse PCR Profiling from IDEXX BioAnalytics.

Blair T.C., Bambina S., Alice A.F., Kramer G.F., Medler T.R., Baird J.R., Broz M.L., Tormoen G.W., Troesch V., Crittenden M.R, & Gough M.J. (2020). Dendritic Cell Maturation Defines Immunological Responsiveness of Tumors to Radiation Therapy. Journal of immunology (Baltimore, Md. : 1950), 204(12), 3416-3424.

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