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Ccr5 pe cy7

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The CCR5-PE-Cy7 is a fluorescently-labeled antibody specific for the CCR5 receptor. It is designed for use in flow cytometry applications to identify and quantify cells expressing the CCR5 receptor.

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Lab products found in correlation

Cd8 fitc, by Thermo Fisher Scientific (2 mentions) Cd3 apc, by Thermo Fisher Scientific (2 mentions) Cd4 pe, by Thermo Fisher Scientific (2 mentions) Cd3 apc cy7, by Thermo Fisher Scientific (2 mentions) Kc57 fitc antibody, by Beckman Coulter (2 mentions) Ccr5 pe, by R&D Systems (2 mentions) Ccr6 percp cy5, by BioLegend (2 mentions) Cd90 alexa fluor 647, by BioLegend (2 mentions) Cd4 fitc, by Thermo Fisher Scientific (2 mentions) Facscalibur, by BD (2 mentions) Facscanto, by BD (2 mentions) Cytofix cytoperm plus kit, by BD (2 mentions) Zombie dye yellow staining, by BioLegend (2 mentions) Cd45 vioblue450, by Cytek Biosciences (2 mentions) Cd3 viogreen, by Miltenyi Biotec (2 mentions) Cd103 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd11c percp cy5, by BioLegend (2 mentions) Hla dr fitc, by Miltenyi Biotec (2 mentions) Dc sign fitc, by R&D Systems (2 mentions) Dc sign pe, by R&D Systems (2 mentions)

6 protocols using ccr5 pe cy7

1

Comprehensive CD4+ T Cell Profiling

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One day post isolation FACS analysis was used to determine the percentages of various CD4+ T cell subsets, like naïve (TN), effector memory (TEM), central memory (TCM), T helper 17 cells (Th17), follicular helper T cells (Tfh) and regulatory T cells (Treg). For this the following antibodies were used: CD4-APC H7 (BD Biosciences, 560158), CD45RA-PE Cy7 (BD Biosciences, 560675), CD45RO-PerCP Cy5.5 (BD Biosciences, 560607), CCR7-APC (BioLegend, 353214), CCR6-PE (BD Biosciences, 559562), CXCR5-PerCP Cy5.5 (BioLegend, 335001), CD127-PE Cy7 (BD Biosciences, 560822), CD25-Horizon V450 (BD Biosciences, 560355). Further, the expression of different receptors on unstimulated as well as stimulated CD4+ T cells was determined using the following antibodies: CD4-APC H7, TCR-CD3-APC H7 (BD Biosciences, 560275), CD28-PerCP Cy5.5 (BD Biosciences, 560685), MHC-I-Horizon V450 (BD Biosciences, 561346), FAS-PE (BD Biosciences, 556641), FAS-L-PE (BioLegend, 306407), PD1-APC (BD Biosciences, 558694), PD1-L-PE Cy7 (BD Biosciences, 558017), TRAIL-PE (BD Biosciences, 550516), CTLA-4-PE (BD Biosciences, 555853), CD69-Horizon V450 (BD Biosciences, 560740), CD25-PE Cy7 (BD Biosciences, 557741), CXCR4-PerCP Cy5.5 (BD Biosciences, 560670) and CCR5-PE Cy7 (BD Biosciences, 557752). Cells were analyzed using the BD FACS Canto II with FACSDiva software.
Heigele A., Joas S., Regensburger K, & Kirchhoff F. (2015). Increased susceptibility of CD4+ T cells from elderly individuals to HIV-1 infection and apoptosis is associated with reduced CD4 and enhanced CXCR4 and FAS surface expression levels. Retrovirology, 12, 86.
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2

Immunophenotyping of Immune Cell Populations

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Isolation of peripheral blood mononuclear cells (PBMCs) and cells from the BM, lung, gut, spleen, and human thymus implant were described previously.28 (link) Peripheral blood- and tissue-derived mononuclear cells were stained with monoclonal antibodies to human CD45-eFluor 450 (HI30, eBioscience), CD3-APC H7 (SK7, Pharmingen), CD4-APC (OKT4, eBioscience), and CD8-PerCP Cy5.5 (SK1, BioLegend), CD19-Brilliant Violet 605 (HIB19, BD Horizon), and CCR5-PECy7 (2D7, Pharmingen). Red blood cells were lysed with red cell lysis buffer after cell surface marker staining. Stained cells were fixed with 1% formaldehyde in PBS and examined with Fortessa flow cytometers (BD Biosciences). The data were analyzed by FlowJo v.10 (Tree Star) software.
Khamaikawin W., Shimizu S., Kamata M., Cortado R., Jung Y., Lam J., Wen J., Kim P., Xie Y., Kim S., Arokium H., Presson A.P., Chen I.S, & An D.S. (2017). Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously Infected with HIV-1. Molecular Therapy. Methods & Clinical Development, 9, 23-32.
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3

HIV Infection Analysis by Flow Cytometry

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Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD8-FITC, CD4-FITC, CD4-PE, CD3-APC, CD3-APC-Cy7 (eBioscience, San Diego, CA), CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR5-PE (R&D Systems, Minneapolis, MN), CCR6-PerCp-Cy5.5, CD90 Alexa Fluor-647 (Biolegend, San Diego, CA). For the HIV-infection experiments, following infection for 6–7 days, intracellular levels of p24 were analyzed as described previously8 (link). Briefly, after surface staining, cells were washed, fixed and permeabilized (20 min) following instructions provided in the Cytofix/Cytoperm Plus kit (BD Biosciences) and stained for intracellular p24 with KC57-FITC antibody (Beckman Coulter; Danvers, MA) for 30 min. Analysis was performed on BD FACSCalibur or BD FACSCanto flow cytometers (BD Biosciences) using FACSdiva software, and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers was measured by the percentage of positive cells and the mean fluorescence intensity (MFI).
Rodriguez-Garcia M., Barr F.D., Crist S.G., Fahey J.V, & Wira C.R. (2014). Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract. Mucosal immunology, 7(6), 1375-1385.
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4

HIV Infection Analysis by Flow Cytometry

Cited 1 time
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Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD8-FITC, CD4-FITC, CD4-PE, CD3-APC, CD3-APC-Cy7 (eBioscience, San Diego, CA), CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR5-PE (R&D Systems, Minneapolis, MN), CCR6-PerCp-Cy5.5, CD90 Alexa Fluor-647 (Biolegend, San Diego, CA). For the HIV-infection experiments, following infection for 6–7 days, intracellular levels of p24 were analyzed as described previously8 (link). Briefly, after surface staining, cells were washed, fixed and permeabilized (20 min) following instructions provided in the Cytofix/Cytoperm Plus kit (BD Biosciences) and stained for intracellular p24 with KC57-FITC antibody (Beckman Coulter; Danvers, MA) for 30 min. Analysis was performed on BD FACSCalibur or BD FACSCanto flow cytometers (BD Biosciences) using FACSdiva software, and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers was measured by the percentage of positive cells and the mean fluorescence intensity (MFI).
Rodriguez-Garcia M., Barr F.D., Crist S.G., Fahey J.V, & Wira C.R. (2014). Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract. Mucosal immunology, 7(6), 1375-1385.
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5

Multicolor Flow Cytometry Analysis

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Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
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6

Multicolor Flow Cytometry Analysis

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Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
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