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Thiobarbituric acid

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Sourced in Ireland, United States

Thiobarbituric acid is a chemical compound used in various laboratory applications. It is a white crystalline solid with a molecular formula of C4H4N2O2S. Thiobarbituric acid is commonly used as a reagent in analytical methods, particularly in the detection and quantification of compounds such as lipid peroxidation products.

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Lab products found in correlation

Trichloroacetic acid, by Merck Group (2 mentions) Rosmarinic acid, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Octanal, by Merck Group (1 mentions) Hexanal, by Merck Group (1 mentions) Potassium iodide, by Merck Group (1 mentions) 2 nonanone, by Merck Group (1 mentions) Ethyl butyrate, by Merck Group (1 mentions) Isooctane, by Merck Group (1 mentions) Isovaleraldehyde, by Merck Group (1 mentions) Starch, by Merck Group (1 mentions) Pentanal, by Merck Group (1 mentions) Heptanal, by Merck Group (1 mentions) 1 pentanol, by Merck Group (1 mentions) 2 heptanone, by Merck Group (1 mentions) N hexane, by Honeywell (1 mentions) 1 1 3 3 tetraethoxypropane, by Merck Group (1 mentions) 2 methyl 3 heptanone, by Merck Group (1 mentions) Carnosic acid, by Extrasynthese (1 mentions) Sodium thiosulfate, by Avantor (1 mentions)

Close spelling variants of this product

2-thiobarbituric acid (2 citations)

2 protocols using thiobarbituric acid

1

Stability of Milk Powder under Antioxidants

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Skim milk powder was collected from a local dairy company, and the same batch was used for the production of all FFMPs. Antioxidant-free refined sunflower oils were purchased from a local store (SuperValu, Dublin, Ireland), and stored in closed containers, in the dark under refrigeration (4 °C) until use.
1,1,3,3-tetraethoxypropane (TEP), trichloroAcetic acid (TCA), starch, potassium iodide, 37% hydrochloric acid (HCl), isooctane, BHA, BHT, rosmarinic acid, 1-pentanol, 2-heptanone, 2-pentanone, 3-octen-2-one, 2, 4-decadienal, hexanal, heptanal, octanal, pentanal, 2-nonenal (E), acetone, 2-nonanone, undecanal 2-methyl-4-pentanol, 2-methyl-3-heptanone, isovaleraldehyde and ethyl butyrate were purchased from Sigma-Aldrich (now Merck, Wicklow, Ireland), whereas carnosol and carnosic acid were obtained from Extrasynthese (Extrasynthese Co., Genay Cedex, France). Thiobarbituric acid (TBA) and sodium thiosulfate were obtained from VWR (VWR International Ltd., Dublin, Ireland). Isopropanol (PrOH) was acquired from Romil (Lennox Laboratory Supplies LTD, Dublin, Ireland) and n-hexane from Honeywell (Honeywell, Dublin, Ireland). Acetic acid was obtained from AppliChem GmbH (Darmstadt, Germany). Milli-Q® water (H2O) (18 mΩ) (Merck Millipore, Molsheim, France) or deionized H2O was used throughout the experiments.
Tzima K., Brunton N.P., McCarthy N.A., Kilcawley K.N., Mannion D.T, & Rai D.K. (2021). The Effect of Carnosol, Carnosic Acid and Rosmarinic Acid on the Oxidative Stability of Fat-Filled Milk Powders throughout Accelerated Oxidation Storage. Antioxidants, 10(5), 762.
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2

Quantifying Lipid Peroxidation via MDA Assay

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The malondialdehyde (MDA) assay to measure lipid peroxidation was carried out as described previously [44 (link)]. Briefly, samples were suspended in 250 μl 0.1% (w/v) trichloroacetic acid (TCA; Sigma-Aldrich, Milwaukee, WI, USA) solution and sonicated briefly (<5 s, 30 kHz). The sonicated solution was centrifuged at 15,000 g for 10 min at 4 °C. Supernatant was collected in a new microcentrifuge tube and mixed with 750 μl 0.5% (w/v) thiobarbituric acid (VWR International, Radnor, PA, USA) diluted in 20% (w/v) TCA. Samples were heated at 95 °C for 20 min and then cooled on ice for 10 min before being centrifuged at 15,000 g for 5 min at 4 °C. Absorbance was measured at 532 nm using a microplate reader. Absorbance values were normalized to protein values obtained from a bicinchoninic acid (BCA, Thermo Fisher Scientific, Waltham, MA, USA) assay and expressed vs. control (WT mouse on STD chow).
Chen T., Hill J.T., Moore T.M., Cheung E.C., Olsen Z.E., Piorczynski T.B., Marriott T.D., Tessem J.S., Walton C.M., Bikman B.T., Hansen J.M, & Thomson D.M. (2020). Lack of skeletal muscle liver kinase B1 alters gene expression, mitochondrial content, inflammation and oxidative stress without affecting high-fat diet-induced obesity or insulin resistance. Biochimica et biophysica acta. Molecular basis of disease, 1866(8), 165805.
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