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Goat anti mouse nkp46 antibody

Manufactured by R&D Systems
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The Goat anti-mouse NKp46 antibody is a tool for the detection and study of the NKp46 protein, which is a natural cytotoxicity receptor expressed on the surface of natural killer (NK) cells. This antibody can be used in applications such as flow cytometry, immunohistochemistry, and immunoprecipitation to identify and analyze NK cells.

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Lab products found in correlation

Signalstain dab substrate kit, by Cell Signaling Technology (1 mentions)

2 protocols using goat anti mouse nkp46 antibody

1

Immunohistochemical Detection of NKp46

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Tissue sections (5 μm) were deparaffinized in xylenes and were rehydrated by passing through serial dilutions of ethanol in distilled water. Heat-induced antigen retrieval was performed in ImmunoActive antigen retrieval solution (Matsunami Glass Ind. Ltd.) with microwave thrice for 5 min. Goat anti-mouse NKp46 antibody (R&D systems) was applied to the sections; then, the sections were incubated overnight at 4°C. After washing with phosphate-buffered saline (PBS), the sections were incubated with goat IgG horseradish peroxidase (HRP) polymer antibody (R&D systems). HRP reacted with the 3, 3’-diaminobenzidine (DAB) substrate using the SignalStain® DAB Substrate kit (Cell Signaling Technology). The sections were counterstained with hematoxylin.
Sato M., Arakaki R., Tawara H., Nagao R., Tanaka H., Tamura K., Kawahito Y., Otsuka K., Ushio A., Tsunematsu T, & Ishimaru N. (2022). Disturbed natural killer cell homeostasis in the salivary gland enhances autoimmune pathology via IFN-γ in a mouse model of primary Sjögren’s syndrome. Frontiers in Medicine, 9, 1036787.
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2

Immunofluorescence Staining of NK Cells

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NK cells were isolated from the spleens of Ly5.1C14R and C57BL/6 mice and centrifuged on lysine coated slides, then fixed and stained with goat anti-mouse NKp46 antibody (R&D Systems, AF2225, 1:100) and rabbit anti-mouse PDI (Cell Signaling Technology, 3501S, 1:100) overnight, followed by AlexaFluor488-conjugated donkey anti-goat IgG (H + L) (ThermoFisher, A-11055, 1:250) and AlexFluor546-conjugated donkey anti-rabbit IgG (H + L) (ThermoFisher, A-10040, 1:200). Images were analyzed using LAS AF (Leica Application Suite – Advanced Fluorescence) software (SI Appendix, Methods).
Almeida F.F., Tognarelli S., Marçais A., Kueh A.J., Friede M.E., Liao Y., Willis S.N., Luong K., Faure F., Mercier F.E., Galluso J., Firth M., Narni-Mancinelli E., Rais B., Scadden D.T., Spallotta F., Weil S., Giannattasio A., Kalensee F., Zöller T., Huntington N.D., Schleicher U., Chiocchetti A.G., Ugolini S., Herold M.J., Shi W., Koch J., Steinle A., Vivier E., Walzer T., Belz G.T, & Ullrich E. (2018). A point mutation in the Ncr1 signal peptide impairs the development of innate lymphoid cell subsets. Oncoimmunology, 7(10), e1475875.
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