Anti myc 9e10

The following commercial antibodies were used in this study: anti-PTEN (1:1,000, western blot [WB]), anti-pAkt (ser 473) (1:1,000, western blot), and anti-Akt (1:1,000, western blot) (Cell Signaling Technology); anti-SNX27 (1:100, immunofluorescence [IF]; 1:1,000, western blot), anti-GLUT1 (1:100, IF), and anti-LAMP1 (1:50, IF) (Abcam); anti-Myc (9E10) (1:1,000, western blot; 1:100, IF), anti-GFP (1:1,000, western blot), and anti-GST (1:5,000, western blot) (Santa Cruz Biotechnology); and anti-MBP (1:10,000, western blot), anti-FLAG (1:10,000, western blot; 1:200, IF), and anti-actin (1:10,000, western blot) (Sigma). Horseradish peroxidase (HRP)conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Jackson Immunological. The 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG)-based glucose uptake assay kit (Cayman Chemical) and lactate calorimetric assay kit (Bio Vision Technologies) were used in the study. Synthetic peptides with the PTEN sequence were purchased from GenScript.

Co-IP and IP were performed as described.^{31 (link)} The primary antibodies anti-TBP (T1827) and anti-actin mAb (A2228) were from Sigma-Aldrich (Shanghai, China). Anti-p65 (#4764), anti-IκBα (#4814s) and anti-cleaved caspase-3 (#9661) antibody were from Cell Signaling Tech (Danvers, MA, USA). Anti-myc (9E10) was from Abcam (Cat: ab32, Mouse monoclonal, Cambridge, MA, USA). Anti-IκBα (phospho-Y42) (Rabbit polyclonal, Cat: BS4736,) and anti-IκBα (phospho-S32/S36) (Rabbit polyclonal, Cat: BS4105) were from Bioworld Tech (St. Louis Park, MN, USA). The PageRuler Prestained Protein Ladder from Thermo Scientific (#26616, Waltham, MA, USA) was used to indicate the target protein. Detection and quantification were performed with the Li-cor Odyssey imaging system and its software (Lincoln, NE, USA).