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8 protocols using infrared spectrophotometer

1

Analytical Techniques for Compound Characterization

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1H NMR was performed on a Bruker Avance 400 MHz. The mass spectrum was recorded on a Waters Q-Tof mass spectrometer. UV absorption spectra were recorded on an Evolution 201 UV-Vis spectrophotometer using quartz cells of 1.0 cm path length. A Cary Eclipse fluorescence spectrophotometer was used to record the fluorescence spectra. FT-IR experiments were performed by using a SHIMADZU Infrared spectrophotometer.
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2

Physicochemical Characterization of Synthesized Materials

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An XPert MPD diffractometer was used to record the X-ray diffraction (XRD) patterns of all synthesized materials. The diffractometer was set up with CuK (λ = 1.5406 Å) radiation, a voltage of 40 kV, and a current of 30 mA. At a wavenumber of 400–4000 cm−1, FT-IR spectra were obtained using an 8400S Shimadzu infrared spectrophotometer. SEM pictures of samples were taken using EDAX advanced microanalysis solutions. FESEM studied surface morphology (FE-SEM, JEOL JIB-4610F). N2 adsorption–desorption was studied using Quantachrome NovaWin Gas Sorption Analyzer. A PerkinElmer Pyris 1 analyzer conducted thermogravimetric analysis (TGA) on a 10 mg sample. Thermograms were taken at 10 °C min−1 air temperatures between 30 and 900 °C. Ultraviolet-visible (UV-vis) spectra recorded on a Thermo Scientific GENESIS 10S UV-vis Spectrophotometer for concentration of dyes after each adsorption.
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3

Electrochemical Determination of Toxic Arsenic

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All melting points reported for the monomers, pre-monomers and model compound are uncorrected and determined on a Gallen-kamp Melting Point apparatus with a digital thermometer type MFB-595-010M. Elemental analyses were estimated by an Elemental Analyses system GmbH, VARIOEL, V2.3 July 1998 CHNS Mode. IR spectra were determined on IR-470, Infrared spectrophotometer, Shimadzu using the KBr pellet technique. Room temperature 1H-NMR spectra were carried out on a varian EM-390-NMR (90 MHz) spectrometer and a GNM-LA 400-MHz NMR spectrophotometer using DMSO or CDCl3 as deuterated solvents and in the presence of TMS as an internal reference. Mass spectra were investigated on a Jeol JMS600 mass spectrometer. I-V method (two electrodes composed onto fabricated GCE) was measured for toxic arsenic ions for PAAP/GCE by using Keithley-Electrometer from USA.
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4

FT-IR Analysis of Zn-MOFs and Curcumin

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FT-IR analysis of Zn-MOFs, dopamine, PDA-CUR-Zn-MOFs, CUR, and CUR-Zn-MOFs was performed by an infrared spectrophotometer (Shimadzu, Kyoto). The samples were mixed with KBr pellets and compressed to a pressure of 1.38 × 103 kPa to form disks. Between 400 and 4000 cm−1, the IR spectra of the formed disks were recorded.
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5

Characterization and Antimicrobial Evaluation

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All chemical
reagents used in this study were of analytical grade (Aldrich, purity
>99%). Melting points were measured with a BUCHI 510 mp apparatus. 1H and 13C NMR spectra were recorded on a Bruker
AC 300 spectrometer operating at 300 MHz for proton and 75.47 for
carbon nuclei. Molecular weights were determined using a JEOL JMS
DX-300 mass spectrometer. Elemental analysis was performed by CNRST
Rabat. Infrared (IR) spectra were acquired on a Shimadzu infrared
spectrophotometer using KBr disks. X-ray diffraction data collection
was carried out on the four-circle Oxford Xcalibur diffractometer
(Mo Kα radiation, λ = 0.71073 Å). The in
vitro
antifungal and antibacterial activities were tested
by the agar diffusion technique.
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6

FTIR Analysis of Drug-Excipient Interactions

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Fourier transform infrared spectrum (FTIR) was performed to ascertain or investigate drug – excipient interaction and to study any incompatibility between the ingredients used in the formulation. Changes in the drug’s distinctive peaks following mixing with the excipients were used to anticipate the incompatibility between the drug and the excipients. FTIR was recorded for the pure drug 5-FU, blank niosomes, 5-fluorouracil loaded niosomes using infrared spectrophotometer (Shimadzu corporation, Japan). The formulations were prepared in KBr disk (2 mg sample/200 mg KBr) with a hydrostatic press at a force of 275790.292 Pa’s for 4 min and the spectrum was produced within the wavelength number of 4000 to 400 cm−1 [18 ].
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7

FTIR Analysis of Fab:DS HIP Complexes

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FTIR analysis of Fab:DS HIP complexes was carried out with an infrared spectrophotometer (Shimadzu Corporation, Kyoto, Japan). The samples were brought into intimate contact with the diamond crystal by applying a loading pressure. The samples were placed on diamond crystal top-plate of Attenuated Total Reflectance (ATR) accessory and scanned between 750–4000 cm−1. Spectra obtained using this device represents an average of 32 individual scan possessing a spectral resolution of 4 cm−1.
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8

Characterization of Organic Compounds

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All the chemical reagents used in this study were of analytical grade (Aldrich, purity > 99%).
Melting points were measured with a BUCHÏ 510 m.p. apparatus. 1 H and 13 C NMR spectra were recorded on a Bruker AC 300 spectrometer operating at 300 MHz for proton and 75.47 for carbon nuclei. Molecular weights were determined using a JEOL JMS DX-300 mass spectrometer. Elemental analysis was performed by Microanalysis Central Service (CNRS).
Infrared (IR) spectra were acquired on a Shimadzu infrared spectrophotometer using the KBr disc technique. X-ray diffraction data collection was carried out on the four-circle Oxford Xcalibur diffractometer (Mo-Kα radiation, λ = 0.71073 Å). The in vitro antibacterial and antifungal activity was tested by the agar diffusion technique.
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