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Hcov oc43 nucleocapsid antibody

Manufactured by Sino Biological
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Sourced in China

The HCoV-OC43 Nucleocapsid antibody is a laboratory reagent used for the detection and study of the HCoV-OC43 virus. It specifically binds to the nucleocapsid protein of the HCoV-OC43 virus, which is a structural protein essential for the virus's replication and assembly.

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Lab products found in correlation

Tcs sp8 sted 3x, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

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HCoV-OC43 (3 citations)

2 protocols using hcov oc43 nucleocapsid antibody

1

Antibody-based EC50 Assay for HCoV-OC43

Cited 1 time
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Antibody-based EC50 assay was performed as described previously (Schirtzinger et al., 2022 (link)). HCoV-OC43 Nucleocapsid were detected with HCoV-OC43 Nucleocapsid antibody (Sino Biological, China).
Zhao L., Qin X., Lin T., Xie F., Yao L., Li Y., Xiong B., Xu Z., Ye Y., Chen H, & Qiu S.X. (2022). Multi-target mechanisms against coronaviruses of constituents from Chinese Dagang Tea revealed by experimental and docking studies. Journal of Ethnopharmacology, 297, 115528.
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2

HCoV-OC43 Infection Immunofluorescence Assay

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Cells were incubated in monolayers on 24 well plates lined with climbing sheets, inoculated with 100 TCID50 of HCoV-OC43 at 37 °C for 2 h. The virus inoculum was then removed and replaced with RPMI1640 medium supplemented with 2% FBS containing desired sample concentration. At 48 hpi, the cells were fixed with 4% PFA/PBS, HCoV-OC43 nucleocapsid and the nucleus were detected with HCoV-OC43 nucleocapsid antibody (Sino Biological) and DAPI (Sigma) respectively. Fluorescence detection was performed using a laser scanning confocal fluorescence microscope (TCS SP8 STED 3X, Leica).
Zhao L., Qin X., Lin T., Xie F., Yao L., Li Y., Xiong B., Xu Z., Ye Y., Chen H, & Qiu S.X. (2022). Multi-target mechanisms against coronaviruses of constituents from Chinese Dagang Tea revealed by experimental and docking studies. Journal of Ethnopharmacology, 297, 115528.
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