High-performance liquid chromatography (HPLC) was used to analyze the CBD content in the CBD-β-CDPM powder and CBD-β-CDPM-NS formulation. The HPLC system included the CBM-20A system controller (Shimadzu Corporation, Tokyo, Japan), LC-30AD pump, SIL-30AC autosampler, SPD-M20A PDA detector, and CTO-20AC column oven. A Shim-pack GIS C18 (150 mm × 4.6 mm, 3 μm) from Shimadzu (Shimadzu Corporation, Tokyo, Japan) at 15 °C was used to perform the separations. A degassed mixture of 70% acetonitrile and 30% ultrapure water made up the mobile phase. The sample or standard CBD substance was dissolved in the mobile phase. The injection volume and flow rate were 10 μL and 1 mL/min, respectively. A spectrophotometer was used to monitor the separation at 207 nm. LC Solution software (Shimadzu Corporation, Tokyo, Japan) was used to process the acquired data. The validation parameters included precision, accuracy, specificity, limit of quantitation, limit of detection, linearity, and robustness. There was no matrix interference of either CBD-β-CDPM powder or CBD-β-CDPM-NS in the chromatographic analysis.
Cbm 20a system controller
Manufactured by Shimadzu
Sourced in Japan
The CBM-20A system controller is a device designed to integrate and control various Shimadzu analytical instruments. It serves as the central command unit, coordinating the operation of connected components and enabling the management of experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Spd m20a, by Shimadzu (6 mentions)
Dgu 20a3 degasser, by Shimadzu (4 mentions)
Lc 30ad pump, by Shimadzu (3 mentions)
Sil 30ac autosampler, by Shimadzu (3 mentions)
Sil 20a autosampler, by Shimadzu (3 mentions)
Lcms 8030, by Shimadzu (3 mentions)
Cto 20a column oven, by Shimadzu (3 mentions)
Cto 20a, by Shimadzu (2 mentions)
Lc 20at pump, by Shimadzu (2 mentions)
Sil 20a ht, by Shimadzu (2 mentions)
Sil 20ac autosampler, by Shimadzu (2 mentions)
Lc 20at, by Shimadzu (2 mentions)
Zorbax eclipse xdb c18, by Agilent Technologies (2 mentions)
Lc 20ad pump, by Shimadzu (2 mentions)
High performance liquid chromatography (hplc), by Shimadzu (2 mentions)
Spd m20a pda detector, by Shimadzu (1 mentions)
Shim pack gis c18, by Shimadzu (1 mentions)
Lc solution software, by Shimadzu (1 mentions)
Lcms 2020 mass detector, by Shimadzu (1 mentions)
Sil 20aht auto injector, by Shimadzu (1 mentions)
30 protocols using cbm 20a system controller
1
CBD Content Analysis in Powder and Nanoparticles
2
HPLC-PDA-MS Analysis of Polyphenolic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We analyzed the fractions and individual polyphenolic compounds using a HPLC–PDA–MS system consisting of a CBM-20A system controller (Shimadzu, Kyoto, Japan), two LC-20AD pumps (Shimadzu, Kyoto, Japan), a DGU-20A3 degasser (Shimadzu, Kyoto, Japan), SIL-20A autosampler (Shimadzu, Kyoto, Japan), an SPD-M20A UV–VIS photodiode array detector (Shimadzu, Kyoto, Japan), and a LCMS-2020 mass detector (Shimadzu, Kyoto, Japan). The compounds were analyzed on a Discovery XR-ODS column (15 × 2.1 mm i.d.; 3.0 μm particle size; Supelco Analytical, Bellefonte, PA, USA) at a flow rate of 0.3 mL/min. The column was thermostated at 40 °C. The mobile phase consisted of 1% aqueous acetic acid (A) and acetonitrile containing 1% of acetic acid (B). We programmed the following gradient steps: 25–35% B (0–6 min), 35–60% B (6–11 min), 60–90% B (11–14 min), 90–25% B (14–16 min), 25% B (16–20 min). The injection volume was 2 μL. The MS settings were as follows: electrospray ionization (ESI), negative and positive ion modes, 150–800 m/z scans, N2 drying gas (10 L/min), nebulizer gas flow 1.5 L/min, interface voltage 3.5 kV, and detector voltage 1.2 kV. We acquired and processed the data using Shimadzu LCMS Solution software (v. 5.42, Kyoto, Japan).
3
HPLC-MS/MS Quantification of Amoxicillin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HPLC system consisted of a model CBM-20A system controller, a model LC-20AD pump, a model SIL-20AHT auto-injector, a model CTO-20A column oven, and a model C-R7A plus integrator (Shimadzu, Kyoto, Japan). A Triple Quadrupole Mass Spectrometer (LCMS-8030, applied Shimadzu, Kyoto, Japan) was used for the LC-MS-MS analyses and detection. The MS was operated in positive ion detection mode [19 (link)]. Nitrogen was used as the nebulizing turbo spray. An octadecylsilane chemically bonded silica chromatographic column (50 × 4.6 mm, 3.5 μm) was used for the separation. The mobile phase was prepared by adding 2 mM glacial acetic acid aqueous solution to methanol (20:80, v/v). The flow rate was 1.0 mL·min-1 with a splitting ratio of 1/3 and the column temperature was 40°C. The injection volume was 10 μL.
The temperature of the vaporizer was set at 400°C. The drying gas (N2) flow rate was 15 L/min. The nebulizer gas (N2) flow rate was 3 L/min. The mass spectrometer was operated at unit mass resolution with a dwell time of 100 ms per transition. Collision energy for amoxicillin was set at -11 V and -20 V for IS. Quantification was performed using multiple reactions monitoring (MRM) of the transition ions m/z 366.00 → m/z 349.10 and m/z 350.00 → m/z 106.10 for amoxicillin and IS respectively.
The temperature of the vaporizer was set at 400°C. The drying gas (N2) flow rate was 15 L/min. The nebulizer gas (N2) flow rate was 3 L/min. The mass spectrometer was operated at unit mass resolution with a dwell time of 100 ms per transition. Collision energy for amoxicillin was set at -11 V and -20 V for IS. Quantification was performed using multiple reactions monitoring (MRM) of the transition ions m/z 366.00 → m/z 349.10 and m/z 350.00 → m/z 106.10 for amoxicillin and IS respectively.
4
LC-MS Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-MS separation was performed in the mobile phase. This phase have solvents A and B in gradient, in which A contained 0.1% (v/v) formic acid in water and B had 0.1% (v/v) formic acid in acetonitrile. Agilent ZORBAX Eclipse XDB-C18 column (2.1×150 mm ×3.5 μm) was used in this procedure. The oven was set at 25 °C, and the volume of injection was 1 μL containing 18 mg/mL in methanol. We used Shimadzu LC-MS 8030 with electrospray ion mass spectrometer (ESI-MS) to monitor the eluent under positive ion mode. Then, we scanned it from 100 to 1,000 mass/number of ions (m/z). ESI was performed by using skimmer 65 V and at a fragmentor voltage of 125 V. Highly pure (99.99%) nitrogen was used as drying gas at 10 L/min flow rate, capillary temperature at 350 °C, and nebulizer at 45 psi. The sample was injected to the mass detector by using the LC-30AD pump, Shimadzu CBM-20A system controller, cooler, and CTO-30 column oven with the SIL-30AC autosampler. The results were validated by running the authentic standard compounds and referring to the literature as in the research of (16 (link)).
5
Yin-Chen-Hao-Tang Bioactive Compound Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Shimadzu UHPLC system consisting of a CBM-20A system controller, LC-20AD XR pumps, DGU-20A3 degasser, SIL-20AC XR auto sampler, and CTO-20A column oven coupled with an electrospray ionization (ESI) interface equipped with an LCMS-8030 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) were utilized for the separation and detection of bioactive compounds in Yin-Chen-Hao-Tang and carbamazepine (IS). The remote-controlled software for the Shimadzu UHPLC-MS/MS system (Kyoto, Japan) was LabSolutions v. 5.60 SP1. Statistical calculations were performed using Microsoft Excel.
6
HPLC Separation of Analytes
7
HPLC Analysis of Citrus Flavonoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The flavonoids were detected by the diode array detector (DAD) using a Shimadzu LC-20AT HPLC system (Shimadzu Corporation, Tokyo, Japan). The system was equipped with a CBM-20A system controller (Shimadzu Corporation, Tokyo, Japan), an LC-20AT pump (Shimadzu Corporation, Tokyo, Japan), a CTD-10ASvp column oven (Shimadzu Corporation, Tokyo, Japan), an SPD-M20A UV-vis detector (Shimadzu Corporation, Tokyo, Japan), a SIL-20A auto injector (Shimadzu Corporation, Tokyo, Japan), a DGU-20A5 degasser (Shimadzu Corporation, Tokyo, Japan), and a Shimadzu LC-solution workstation (Shimadzu Corporation, Tokyo, Japan). Compounds were separated using an Agilent ZORBAX SB C18 column (4.6 mm × 250 mm, 5 μm). The solvent system consisted of acetonitrile (A) and water (B). The gradient elution program was as follows: 19% A for 0–10 min, 19–50% A for 10–25 min, and 50–56% A for 25–40 min. The flow rate was 1.0 mL/min. The column temperature was kept at 35 °C. The injection volume was 10 μL. The effluents were monitored based on the maximum absorption wavelength at 284 nm for hesperidin and 330 nm for nobiletin and tangeretin.
8
HPLC Analysis of Hst Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the chromatographic analysis, a standard solution (100 μg/mL) of Hst (C16H14O6; ≥98.0%) in acetonitrile was prepared. The HPLC analyses were performed using an HPLC system that consisted of a degasser DGU-20A3, a SIL-20A HT auto injector, two LC-20AD pumps, an SPDM20A photodiode array detector (DAD), a CTO-20A column oven and a CBM-20A system controller (Shimadzu Co., Kyoto, Japan), equipped with a column of 4.6×150 mm2 (5 μm particle size). The injection volume was 20 μL with a flow rate of 1.0 mL/min. The mobile phase consisted of acetonitrile/water/acetic acid 0.1% (50:49:1, v/v/v). The detector was set at 290 nm for acquiring chromatograms.
9
Quantitative Analysis of Fermentation Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of methanol, formate, and acetate in the culture supernatant were measured. A 10 mL of culture was collected from the column every hour, three times in total, at the time just before the peristaltic pump worked. Each sample was immediately filtered with a 0.22-μm Millex-GP filter (Merck, Darmstadt, Germany) that was pre-washed three times with distilled water to remove any solutes from the filter. Methanol was measured by using an Agilent 6890N gas chromatograph coupled with an Agilent 7694 headspace sampler (Agilent, Santa Clara, CA, USA). Formate and acetate were derivatized with 2-nitrophenylhydrazine using the fatty acid analysis kit (YMC, Kyoto, Japan) and measured by a high-performance liquid chromatography (HPLC) system with a CBM-20A system controller and an SPD-10A UV/VIS detector (Shimadzu, Kyoto, Japan) on a Nova-Pak C18 column (3.9 by 150 mm; Waters, Milford, MA, USA) at 45°C. The eluents were acetonitrile containing 0.1% trifluoroacetic acid (A) and distilled water (B), and the following gradient elution program was run: 1.0 mL/min with 7.5% A in 45 min, 100% A in 10 min, and 7.5% A in 10 min. The medium was used as a negative control. The independent sampling and measurement were repeated three times.
10
Serum Retinol Measurement via HPLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum retinol was measured using a high-performance liquid chromatography LC-20AT model equipped with an SIL- 20AC automatic injector, a CBM-20A system controller, and an SPD-M20A controller diode array detector (Shimadzu Inc. Tokyo, Japan). We defined “VitA deficiency” as VitA levels below 0.35 µmol/L, “low VitA levels” as between 0.35 and 0.70 µmol/L, and “sufficient VitA levels” as below 0.70 µmol/L27 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!