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Powerlab data acquisition and analysis system

Manufactured by ADInstruments
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Sourced in Australia, United States

The PowerLab data acquisition and analysis system is a versatile hardware platform designed for recording and analyzing physiological signals. It offers high-quality data capture capabilities, allowing users to monitor and collect a range of biological measurements. The system is compatible with a variety of sensors and transducers, enabling researchers and educators to gather valuable data for their applications.

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Lab products found in correlation

Bioelectric amplifier, by ADInstruments (3 mentions) Needle electrode, by ADInstruments (3 mentions) Multiscango enzyme standard instrument, by Thermo Fisher Scientific (3 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) Cytochrome c, by Merck Group (2 mentions) Taurine, by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Imidazole, by Merck Group (2 mentions) Adp potassium salt, by Merck Group (2 mentions) Lactobionate, by Merck Group (2 mentions) Powerlab signal acquisition and analysis system, by Thermo Fisher Scientific (2 mentions) Rotenone, by Merck Group (2 mentions) Malate, by Merck Group (2 mentions) Succinate, by Merck Group (2 mentions) Glutamate, by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Atp na2, by Merck Group (2 mentions) High resolution respirometry, by Oroboros (2 mentions)

Close spelling variants of this product

PowerLab (853 citations) PowerLab system (368 citations) Powerlab acquisition system (17 citations) Data acquisition system (9 citations)

8 protocols using powerlab data acquisition and analysis system

1

Oxidative Stress Biomarkers and Inflammation

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The main reagents used in the present study are listed below. ROS kits were provided by Nanjings Mr Ng biological technology Co., Ltd. The CK-MB kit was provided by Wuhan huamei biological engineering Co., Ltd. IL-6, CRP, and TNF-α kits were purchased from the Wuhan optimal, born trade Co., Ltd. TXNIP, TRX, NF-ĸ Bp65, NLRP3, and Caspase-1 antibody were obtained from Abcam Trading (Shanghai) Company Ltd. β-Actin antibody was supplied by Beijing zhongshan jinqiao biotechnology Co., Ltd.
The following main instruments were used in the present study: a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, United States), a Sigma 3k15 high-speed refrigerated centrifuge (SIGMA, Germany), a pressure volume catheter (SPR-838, Millar company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), vertical electrophoresis system (BIO-TEK, USA), transfer electrophoresis system (BIO-TEK, USA), a gel imaging system (Bio Spectrum), an image analysis system (Image-Pro Plus 4.1), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), and a needle electrode (AD Instruments, Australia).
Li Y., Xu P., Wang Y., Zhang J., Yang M., Chang Y., Zheng P., Huang H, & Cao X. (2020). Different Intensity Exercise Preconditions Affect Cardiac Function of Exhausted Rats through Regulating TXNIP/TRX/NF-ĸBp65/NLRP3 Inflammatory Pathways. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 5809298.
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2

Cardiac Function Assessment in Sepsis

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Then, 18 h after the operation, a hypodynamic stage of sepsis [27 (link)], the cardiac function of rats was assayed. After anesthesia by 3% isoflurane inhalation, the LVs were intubated via the right common carotid artery by a catheter filled with heparin saline (500 U/ml) to measure mean arterial blood pressure (MABP) and LV pressure. Left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximum rise/fall rate of LV pressure (±dp/dtmax) were recorded by the PowerLab Data Acquisition and Analysis System (ADInstruments, Australia).
Han X., Chen D., Liufu N., Ji F., Zeng Q., Yao W, & Cao M. (2020). MG53 Protects against Sepsis-Induced Myocardial Dysfunction by Upregulating Peroxisome Proliferator-Activated Receptor-α. Oxidative Medicine and Cellular Longevity, 2020, 7413693.
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3

Heating-Induced Systolic Blood Pressure

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The rats in the experimental group were placed in a folded heating blanket, with the temperature set to 40°C. A towel was placed between the animals and the warm blanket to avoid burns. The core temperature (represented by the rectal temperature) was monitored by the PowerLab Data Acquisition and Analysis System (AD Instruments, Shanghai, China) every 5 min. The point at which the systolic blood pressure (represented by the caudal artery systolic pressure) decreased from its peak value was taken as the onset of HS. The rats were removed immediately from the heating blanket and returned to room temperature (25°C).
Chen X.J., Tang Z.Z., Zhu G.G., Cheng Q., Zhang W.K., Li H.M., Fu W, & Lu Q.P. (2017). JNK signaling is required for the MIP-1α-associated regulation of Kupffer cells in the heat stroke response. Molecular Medicine Reports, 16(3), 2389-2396.
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4

Cardiac Function Assessment in Rats

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The cardiac function of rats was measured at the end of reperfusion. The left ventricular systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP) were detected using the PowerLab Data Acquisition and Analysis System (ADInstruments, Australia). The fractional shortening (FS) and ejection fraction (EF) were detected using HD15 Color Doppler Ultrasound Diagnostic System (Phillips, Netherlands). The specific operations were carried out in accordance with the instructions of instruments
Wei D., Xu H., Gai X, & Jiang Y. (2019). Astragaloside IV alleviates myocardial ischemia-reperfusion injury in rats through regulating PI3K/AKT/GSK-3β signaling pathways. Acta Cirúrgica Brasileira, 34(7), e201900708.
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5

Cardiac Biomarkers Measurement Protocols

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The main reagents used in the present study are listed below. The cardiac troponin I (cTn-I) and N terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Cloud-Clone Corp. (Wuhan, China). ADP potassium salt, Cytochrome c, Lactobionate, ATP Na2, Na2Phosphocreatine, EGTA, Ascorbic acid, Taurine, Imidazole, DTT, HEPES, MES, Glutamate, Malate, Succinate, TMPD, Antimycin A and Rotenone were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The following main instruments were used in the present study: An animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
Ping Z., Qiu W., Yang M., Zhang X., Wang D., Xu P., Wang Z, & Cao X. (2021). Optimization of different intensities of exercise preconditioning in protecting exhausted exercise induced heart injury in rats. Sports Medicine and Health Science, 3(4), 218-227.
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6

Induction and Treatment of Cardiac Hyperthyroidism in Rats

Cited 1 time
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The thyrotoxicosis rat model was induced by daily intraperitoneal (i.p.) injection of levothyroxine (T4, 100 µg/kg/day) for 28 days35 (link).
Sixty adult male Wistar rats were randomly divided into four equal groups of 15: (I) a Control group receiving corn oil vehicle (2 mL/kg/day; p.o.), (II) a cardiac hyperthyroid (CH) group receiving T4 (100 µg/kg/day, i.p.) plus corn oil (2 mL/kg/day, p.o.), (III) a cardiac hyperthyroid plus sacubitril/valsartan (CH + LCZ696) group receiving T4 (100 µg/kg/day, i.p.) for 28 days followed by LCZ696 (60 mg/kg/day) for 14 days, and (IV) a cardiac hyperthyroid plus valsartan group (CH + VAL) receiving T4 (100 µg/kg/day, i.p.) for 28 days15 (link) followed by valsartan (30 mg/kg/day) for 14 days13 (link).
At the end of the treatment period, animals were weighed using an electronic balance (BXX 40; BOECO, Germany) and then anesthetized. Hemodynamic parameters were measured under anesthesia using the Power Lab data acquisition and analysis system (AD Instruments, Australia). The thorax was then opened and the heart rapidly removed, weighed, and processed for molecular, histological, or biochemical assessments as described.
Khamis T., Alsemeh A.E, & Abdullah D.M. (2022). Sacubitril/valsartan (LCZ696) ameliorates hyperthyroid-induced cardiac hypertrophy in male rats through modulation of miR-377, let-7 b, autophagy, and fibrotic signaling pathways. Scientific Reports, 12, 14654.
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7

Cardiac Biomarker Detection Assay

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The main reagents used in the present study are listed below. The cardiac troponin I (cTnI) and N-terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Shanghai Kmaels Biologic Technology Co., Ltd., ADP potassium salt, cytochrome c, lactobionate, ATP-Na2, Na2Phosphocreatine, EGTA, ascorbic acid, taurine, imidazole, DTT, HEPES, MES, glutamate, malate, succinate, TMPD, antimycin A and rotenone were purchased from Sigma (USA). The following main instruments were used in the present study: an animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
Su Y., Wang Y., Xu P., Sun Y., Ping Z., Huang H, & Cao X. (2021). Study on the time-effectiveness of exercise preconditioning on heart protection in exhausted rats. The Chinese journal of physiology, 64(2).
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8

Measuring Right Ventricular Pressure

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RVSP equals to pulmonary artery systolic pressure (PASP) when the right ventricular outflow obstruction is absent according to the modified Bernoulli equation. RVSP was measured by inserting an eight-gauge needle with high-fidelity pressure transducer to right ventricle. The value of RVSP was recorded and analyzed by Power Lab Data Acquisition and Analysis System (PL 3504, AD Instruments, Australia). RVHI is identical to right ventricle over the sum of left ventricle plus septum (RV/LV + S) according to the Fulton index measurement.
Song M., Wang J., Sun Y., Pang J., Li X., Liu Y., Zhou Y., Yang P., Fan T., Liu Y., Li Z., Qi X., Li B., Zhang X., Wang J, & Wang C. (2021). Inhibition of gasdermin D-dependent pyroptosis attenuates the progression of silica-induced pulmonary inflammation and fibrosis. Acta Pharmaceutica Sinica. B, 12(3), 1213-1224.
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