L arginine

THP-1 cells (TIB-202TM) were from ATCC. RPMI 1640 media and Fetal bovine serum (FBS) were purchased from Gibco. HEPES, penicillin/streptomycin, L-glutamine, Hanks’ balanced salt solution, M199 medium, folic acid, hemin, adenosine, Pepstatin A, Phorbol 12-myristate 13-acetate (PMA), PreScission enzyme (GE27-0843-01), and Amicon® Ultra-2 centrifugal filter devices (UFC200324) were purchased from Millipore Sigma. Anti-Ago1 (5053), anti-Ago2 (2897), anti-Ago3 (5054), anti-Lamin A/C (2032), anti-Calreticulin (12238), and anti-Calnexin (2679) were purchased from Cell Signaling. Anti-GST (SC-138), anti-HSC70 (HSP70) (SC-7298), and anti-Actin (SC-47778) were from Santa Cruz biotechnology. Anti-GAPDH (G041) was from Abm, and anti TRBP (Ab42018) was purchased from Abcam. RPMI 1640 media lacking L-glutamine, L-arginine, and L-lysine was purchased from Caisson labs. North2South Hybridization buffer (37549), North2South Hybridization Stringency Wash Buffer (37555), and Chemiluminescent Nucleic Acid Detection Module kit (89880) were purchased from ThermoScientific.

PC-3 cells were obtained from ATCC and cultured in DMEM supplemented with 10% FBS. The following antibodies were used: BIOTIN (Cat. 5597) and GAPDH (Cat. 2118) were from Cell Signaling; CLU (Cat. 05-354) and ACTIN (Cat. MAB1501) were from Millipore; FOS (Cat. sc-166940), TIA-1 (sc-1751) and HSPA1A (cat. sc-32239) were from Santa Cruz Biotech; JUN (Cat. 610326) and G3BP1 (Cat. 611127BD) were from BD Biosciences; BAX (Cat. ab32503, ab77566), APX2 (Cat. ab222414) and YB-1 (Cat. ab76149) were from Abcam; HIF1A (Cat. NB100-131) was from Novus; Fluorescently labeled secondary antibodies (mouse, Alexa Fluor 488/594; rabbit, Alexa Fluor 488/594; and goat, Alexa Fluor 488/594), TRIzol, RNAiMAX transfection reagent, Dynabeads M-280 Streptavidin, DMEM, FBS, Click-iT Protein Reaction Buffer Kit, BIOTIN-alkyne and l-azidohomoalanine (AHA) were from Life Technologies; Trolox, BIOTIN-tyramide, sodium ascorbate, sodium deoxycholate and cycloheximide were from Sigma; DMEM without l-lysine and l-arginine was from Caisson Labs (USA); ¹³C₆-arginine and D4-lysine were from Silantes; FluorSave was from Merck; and G3BP1-APEX and CTRL-APEX constructs were custom made from GenScript.