Sc 81714

8-μm tissue sections were cut by cryo-sectioning and stained with conjugated or purified antibodies. Purified antibodies were detected using secondary antibodies. The antibodies included MECA-79 (sc-19602, SCBT), anti-CD11c (117301, BioLegend), anti-CD11b (101202, BioLegend), anti-LYVE1 (ab14917, Abcam), anti-αSMA (19245S, CST), anti-CD31 (14-0311-82, Invitrogen), anti-Collagen I (ab34710, Abcam), anti-Collagen IV (NBP1-91258, Novus), anti-Laminin (ab11575, Abcam), anti-pan-cytokeratin (AE1/AE3, sc-81714, SCBT). DAPI (VECTASHIELD, Vector Laboratories) was used to counterstain the cell nuclei. The stained tissue sections were visualized using an EVOS™ FL Auto 2 Imaging System (Thermo Fisher Scientific). Quantification was performed on 2–3 sections from at least 3 separate mice using image analysis software Celleste (Invitrogen) and ImageJ (NCBI, 1.8.0_112).

5μm sections of tissue were cut by cryosectioning and stained with conjugated or purified antibodies. Purified antibodies were detected using secondary antibodies. The antibodies used include anti-Sb9 (PA5-51038, Invitrogen), anti-mouse GrB (16G6, #14-8822-82, Invitrogen), anti-human GrB (2C5, sc-8022, SCBT), anti-human GrB (B18.1, NBP1-97525, Novus), anti-MelanA (ab51061, Ab-cam), anti-α-SMA (D4K9N, #19245S, CST), anti-cleaved caspase-3 (Asp175, #9661, CST), anti-Collagen I (NB600-408, Novus), anti-Fibronectin (NBP1-91258, Novus), anti-PDGFR-α (APA5, ab90967, Abcam), anti-PDGFRβ (APB5, #14-1402-82, Invitrogen), anti-pan-cytokeratin (AE1/AE3, sc-81714, SCBT), anti-FAP (ab53066, Abcam), anti-FSP-1 (S100A4, ABF32, Millipore), FITC anti-CD11b (M1/70, #101206, Biolegend), FITC anti-Gr-1 (RB6-8C5, #108417, Biolegend), and anti-Ly-6C (HK1.4, #128012, Biolegend). DAPI (VECTASHIELD, Vector Laboratories) was used to counterstain the cell nuclei. The stained tissue sections were visualized using an EVOSTM FL Auto 2 Imaging System (Thermo Fisher Scientific) for whole images and a fluorescence confocal microscope (Nikon) for high-resolution images. Quantification was performed on 2–3 sections from at least 3 separate mice using image analysis softwares Celleste (Invitrogen) and ImageJ (NCBI, 1.8.0_112).