Dual-color immunofluorescence was performed to characterize Mo-DC, using the following panel of mAbs: PE- or FITC-conjugated anti-human HLA-DR (clone L242, BD Pharmingen); PE- or FITC-conjugated anti-human CD1a (clone HI149, Biolegend); PE- or FITC-conjugated anti-human CD86 (clone IT2.2, Biolegend); FITC-conjugated anti-human CD40 (clone HB14, Biolegend); PE-conjugated anti-human CD80 (clone 2D10, Biolegend); FITC-conjugated anti-human CD83 (clone HB15e, Biolegend); PE-conjugated anti-human CCR7 (clone 150503, BD Pharmingen). Negative controls were isotype-matched irrelevant mAbs (BD Pharmingen, Biolegend). Cells were analyzed by using FACScan equipment (Becton Dickinson). A minimum of 10000 events was collected in list mode on FACScan software.
Fitc conjugated anti human cd40 clone hb14
Manufactured by BioLegend
FITC-conjugated anti-human CD40 (clone HB14) is a monoclonal antibody that binds to the CD40 antigen on human cells. The antibody is conjugated with fluorescein isothiocyanate (FITC), which enables it to be detected by flow cytometry or other fluorescence-based applications.
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Lab products found in correlation
Pe or fitc conjugated anti human hla dr clone l242, by BD (2 mentions)
Pe or fitc conjugated anti human cd1a clone hi149, by BioLegend (2 mentions)
Pe or fitc conjugated anti human cd86 clone it2, by BioLegend (2 mentions)
Pe conjugated anti human cd80 clone 2d10, by BioLegend (2 mentions)
Fitc conjugated anti human cd83 clone hb15e, by BioLegend (2 mentions)
Pe conjugated anti human ccr7 clone 150503, by BD (2 mentions)
Facscan equipment, by BD (2 mentions)
2 protocols using fitc conjugated anti human cd40 clone hb14
1
Phenotypic Characterization of Mo-DC
2
Multicolor Flow Cytometry for Immune Cell Phenotyping
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Dual-color immunofluorescence was performed using the following panel of mAbs: PE- or FITC-conjugated anti-human HLA-DR (clone L242, BD Pharmingen); PE- or FITC-conjugated anti-human CD1a (clone HI149, Biolegend); PE- or FITC-conjugated anti-human CD86 (clone IT2.2, Biolegend); PE-conjugated anti-human CD80 (clone 2D10, Biolegend); PE- or FITC-conjugated anti-human CD14 (clone HCD14, Biolegend); FITC-conjugated anti-human CD83 (clone HB15e, Biolegend); FITC-conjugated anti-human CD40 (clone HB14, Biolegend); PE-conjugated anti-human CCR7 (clone 150503, BD Pharmingen). Negative controls were isotype-matched irrelevant mAbs (BD Pharmingen, Biolegend). Cells were analyzed by using FACScan equipment or C6 Accuri equipment (Becton Dickinson). A minimum of 10,000 events was collected in list mode on FACScan software.
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