Omental tissue, obtained from female patients undergoing surgery for benign conditions, was cut into pieces weighing 300 mg and cultured in DMEM containing 10% FBS in 6 well dishes. GFP-expressing HeyA8 cells were added to the omentum pieces and allowed to colonize for up to 7 days. Medium was changed every day. At the end point the cancer cells colonizing the omentum were imaged with an Axio-observer A1 fluorescent microscope using 1.25× objective (Carl Zeiss). Thereafter, the omentum pieces were digested with Liberase (Roche) and the cancer cell growth was quantified by measuring their fluorescence in a plate reader (Synergy HT, BioTek), orcancer cells were isolated by FACS for miR-193b qPCR.
Axio observer a1 fluorescent microscope
Manufactured by Zeiss
The Axio-observer A1 is a fluorescent microscope designed for basic research and educational purposes. It features LED illumination, a motorized stage, and the capability to capture fluorescent images. The Axio-observer A1 provides high-quality optical performance for visualizing and analyzing fluorescently labeled specimens.
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Lab products found in correlation
6 protocols using axio observer a1 fluorescent microscope
1
Omental Colonization by GFP-HeyA8 Cells
2
Omentum Invasion Assay Protocol
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Cellular invasion through the surface of the omentum was assayed in vitro by first assembling the 3D culture on 8 μm pore size Fluoroblocktranswell inserts in 24 well plates. GFP-expressing OvCa cells were then seeded on the 3D culture in the transwell insert in 200 μl serum-free DMEM. DMEM with 10% FBS served as a chemoattractant in the lower chamber. Cells were allowed to invade for 16 h and then were fixed with 4% paraformaldehyde. The fluorescent cancer cells that had invaded were imaged (5 fields/insert) using an Axio-observer A1 fluorescent microscope (Carl Zeiss).
3
Transwell Migration Assay for OvCa Cells
4
Omentum Invasion Assay Protocol
5
Omental Colonization by GFP-HeyA8 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Omental tissue, obtained from female patients undergoing surgery for benign conditions, was cut into pieces weighing 300 mg and cultured in DMEM containing 10% FBS in 6 well dishes. GFP-expressing HeyA8 cells were added to the omentum pieces and allowed to colonize for up to 7 days. Medium was changed every day. At the end point the cancer cells colonizing the omentum were imaged with an Axio-observer A1 fluorescent microscope using 1.25× objective (Carl Zeiss). Thereafter, the omentum pieces were digested with Liberase (Roche) and the cancer cell growth was quantified by measuring their fluorescence in a plate reader (Synergy HT, BioTek), orcancer cells were isolated by FACS for miR-193b qPCR.
6
Transwell Migration Assay for OvCa Cells
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Transwell Migration assays were conducted using 8 μm pore size inserts (BD, Falcon). GFP-expressing OvCa cells transfected with pre- or anti-miR-193b were added to the upper chamber in 200 μl DMEM and allowed to migrate for 3 h at 37°C. DMEM with 10% FBS was used as a chemoattractant in the lower chamber. Cells were fixed in 4% paraformaldehyde and imaged (5 fields/insert) using an Axio-observer A1 fluorescent microscope (Carl Zeiss).
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