Caspase-3 immunohistochemistry was performed as previously described method [4 (link),20 (link)]. The sections were incubated overnight with a rabbit anti–caspase-3 antibody (1:500; Santa Cruz Biotechnology, Dallas, TX, USA), and then incubated for another 1 hour with the biotinylated rabbit secondary antibody. The bound secondary antibody was then amplified using a Vector Elite ABC kit (Vector Laboratories). The antibody-biotinavidin-peroxidase complex was visualized using 0.02% DAB.
Rabbit anti caspase 3 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit anti–caspase-3 antibody is a laboratory reagent used to detect the presence and activity of caspase-3, a key enzyme involved in apoptosis or programmed cell death. This antibody can be used in various immunoassay techniques to study the role of caspase-3 in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Vector elite abc kit, by Vector Laboratories (2 mentions)
Mouse anti neun antibody, by Merck Group (1 mentions)
Mouse anti dcx antibody, by Santa Cruz Biotechnology (1 mentions)
Permount, by Thermo Fisher Scientific (1 mentions)
Biotinylated secondary antibody, by Vector Laboratories (1 mentions)
Tissue protein extraction reagent, by Thermo Fisher Scientific (1 mentions)
Image studio lite analysis software, by LI COR (1 mentions)
Mouse anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Odyssey fc imaging system, by LI COR (1 mentions)
3 protocols using rabbit anti caspase 3 antibody
1
Caspase-3 Immunohistochemistry Protocol
2
Immunohistochemical Analysis of Neural Markers
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Immunohistochemistry for NeuN, caspase-3, and DCX were performed, as the previously described method (Kim et al., 2017 ; Lee et al., 2016 (link)). Six sections on average were selected in each brain region spanning from Bregma −2.50 mm to −4.50 mm. The sections were incubated overnight with mouse anti-NeuN antibody (1:500; EMD Millipore Corp., Billerica, MA, USA), rabbit anti-caspase-3 antibody (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and mouse anti-DCX antibody (1:500; Santa Cruz Biotechnology), and then they were incubated for another 2 hr with the biotinylated secondary antibody (1:200; Vector Laboratories). The bound secondary antibody was then amplified using a Vector Elite ABC kit (Vector Laboratories). The antibody-biotin-avidin-peroxidase complex was visualized using 0.02% DAB. The sections were finally mounted onto gelatin-coated slides. The slides were air-dried overnight at room temperature, and the coverslips were mounted using Permount (Fisher Scientific).
3
Quantitative Protein Analysis in Zebrafish
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Total protein was extracted from zebrafish embryos at 120hpf using the tissue protein extraction reagent (Thermo Scientific, UK) as described in the manufacturer's protocol, and centrifuged at 13000rpm for 10 minutes. The supernatant was transferred to a 1.5ml eppendorf tube and protein concentration was measured. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 5% milk powder in Tris-Buffered Saline-Tween 20 buffer for 1 hour at room temperature and then incubated with a primary antibody (mouse anti-GAPDH antibody, Santa Cruz Biotechnology, Inc., Cat. Sc-32233 or rabbit anti-caspase3 antibody, antibodies online.com, Cat. ABIN1883652 in 1:1000 dilution) overnight at 4°C. The membrane was washed and then incubated with a secondary antibody (goat anti-rabbit antibody, Cat. 926-32211 or donkey anti-mouse antibody, Cat. 926-68072 (LI-COR Biosciences, USA) in 1:10000 dilution). The signals were visualized by the LI-COR Odyssey FC Imaging System and quantified using Image Studio™ Lite analysis software (LI-COR).
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