The largest database of trusted experimental protocols

Nis elements br v4

Manufactured by National Instruments

NIS Elements BR v4.5 is a comprehensive software suite for advanced microscopy imaging and analysis. It provides a powerful platform for acquiring, processing, and analyzing images from a wide range of microscopy techniques, including fluorescence, brightfield, and confocal microscopy. The software supports a variety of image formats and includes tools for image enhancement, quantification, and 3D visualization.

Automatically generated - may contain errors

5 protocols using nis elements br v4

1

Detailed Morphometric Measurements for Staphylinidae

Check if the same lab product or an alternative is used in the 5 most similar protocols
All measurements were made using a live measurement module within NIS Elements BR v4.5. Measurements were taken as listed below, but only proportional (HW/HL, PW/PL, EW/EL, PW/HW) and forebody measurements are stated directly in descriptions. Total body length is generally difficult to standardize for Staphylinidae and was not measured due to the contractile nature of the abdomen.
HL Head Length, at middle, from the anterior margin of frons to the nuchal ridge.
HW Head Width, the greatest width, including the eyes.
PL Pronotum Length, at middle.
PW Pronotum Width, greatest width.
EL Elytral Length, greatest length taken from level of the anterior most large, lateral macroseta to apex of elytra. EL approximates the length of the elytra not covered by the pronotum and therefore contributing to the forebody length.
EW Elytral Width, greatest width.
ForebodyHL + PL + EL.
+ Open protocol
+ Expand
2

Standardized Insect Morphometrics for Staphylinidae

Check if the same lab product or an alternative is used in the 5 most similar protocols
All measurements were made using a live measurement module within NIS Elements BR v. 4.5. Measurements were taken as listed below, but only proportional (HW/HL, PW/PL, EW/EL, PW/HW) and forebody measurements were stated directly in descriptions. Total body length is generally difficult to standardize for Staphylinidae and was not measured due to the contractile nature of the abdomen.
HL Head Length, at middle, from the anterior margin of frons to the nuchal ridge;
HW Head Width, the greatest width, including the eyes;
PL Pronotum Length, at middle;
PW Pronotum Width, greatest width;
EL Elytral Length, greatest length taken from level of the anterior most large, lateral macroseta to apex of elytra. EL approximates the length of the elytra not covered by the pronotum and therefore contributing to the forebody length;
EW Elytral Width, greatest width;
ESut Elytral Suture, apex of the scutellum to the apex of the elytra
ForebodyHL + PL + EL.
+ Open protocol
+ Expand
3

Specimen Preservation and Genital Dissection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All specimens were examined using a Nikon 745T stereomicroscope. To allow for the routine dissection of the terminal abdominal segments (including the aedeagus), distilled water was applied directly to the tip of the abdomen using a fine paintbrush. As a precaution against DNA degradation, specimens examined in the present study were never subjected to high ambient humidity relaxing chambers or entirely submersed in water. This was in direct contrast to the specimens dissected for Brunke and Solodovnikov (2014) , which may be less suitable for future amplification of DNA. Genitalia were cleared in a 10% potassium hydroxide solution and then washed with distilled water, then with 70% alcohol and finally placed in glycerin for observation. Genitalia were placed in glycerin filled vials for long-term storage, which were pinned with their respective specimen.
Line illustrations were performed in Adobe Illustrator CS6 based on photographs. Photomontage was accomplished using a motorized Nikon SMZ25 microscope and NIS Elements BR v4.5. Photos were processed in Adobe Photoshop CS6. Distribution maps were created using QGIS 2.18 as in Brunke and Solodovnikov (2014) .
+ Open protocol
+ Expand
4

Stereomicroscopic Imaging and Illustration

Check if the same lab product or an alternative is used in the 5 most similar protocols
All specimens were examined dry using a Nikon SMZ25 stereomicroscope. Genitalia and terminal segments of the abdomen were dissected and placed in glycerin filled vials, pinned with their respective specimens. Line illustrations were made from standard images and then digitally inked in Adobe Illustrator CC-2020. All imaging, including photomontage was accomplished using a motorized Nikon SMZ25 microscope and NIS Elements BR v4.5. Photos were post-processed in Adobe Photoshop CC-2020.
+ Open protocol
+ Expand
5

Specimen Examination and Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All specimens were examined dry using a Nikon SMZ25 stereomicroscope. Genitalia and terminal segments of the abdomen were dissected and placed in glycerin filled vials, pinned with their respective specimens. Line illustrations were made from standard images and then digitally inked in Adobe Illustrator CC-2021. All imaging, including photomontage was accomplished using a motorized Nikon SMZ25 microscope and NIS Elements BR v. 4.5. Photos were post-processed in Adobe Photoshop CC-2021.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!