Superoxide dismutase

Manufactured by Abcam

Sourced in United States, United Kingdom

Superoxide dismutase is an enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. It is an important antioxidant defense in nearly all cells exposed to oxygen.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using superoxide dismutase

Antioxidant Enzyme Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The list of the primary and secondary antibodies and their dilutions is given in Table 1. Superoxide dismutase, Anti-Catalase, Anti-Glutathione Reductase from Abcam (Cambridge, MA, USA), while FITC, 1:1000 and TRITC were bought from Jackson ImmunoResearch Laboratories (Ely, Cambridgeshire, UK).

Adeghate E., D’Souza C.M., Saeed Z., Al Jaberi S., Tariq S., Kalász H., Tekes K, & Adeghate E.A. (2021). Nociceptin Increases Antioxidant Expression in the Kidney, Liver and Brain of Diabetic Rats. Biology, 10(7), 621.

+ Open protocol

+ Expand

Evaluating Apelin-13's Antioxidant Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apelin-13 was purchased from Sigma Aldrich (St Louis, MO). Malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and enzyme-linked immunosorbent assay (ELISA) kits were bought from Abcam Inc. (Cambridge, MA). Goat anti-rat endothelial nitric oxide synthase (eNOS), Bax primary antibody, mouse anti-rat Bcl-2 primary antibody (Santa Cruz), mouse anti-goat secondary antibody, and rabbit anti-mouse secondary antibody were provided by Santa Cruz Biotechnology (Dallas, TX).

Xu Z, & Li Z. (2020). Experimental Study on the Role of Apelin-13 in Alleviating Spinal Cord Ischemia Reperfusion Injury Through Suppressing Autophagy. Drug Design, Development and Therapy, 14, 1571-1581.

+ Open protocol

+ Expand

Evaluating Oxidative Stress Markers in Bladder Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Superoxide dismutase (SOD; Abcam, Cambridge, UK) activity, reported as U/mg tissue, was determined by measuring enzyme-mediated inhibition of nitroblue tetrazolium reduction [18 (link)]. One unit of SOD was defined as the enzyme activity that inhibited nitroblue tetrazolium reduction by 50%. Malondialdehyde (MDA; Abcam, Cambridge, UK) levels in bladder tissues, expressed as nmol/g tissue, were analyzed using a method based on the reaction of MDA with thiobarbituric acid at 95°C [19 (link)], which produces a pink pigment with maximum absorption at 532 nm. Bladder injury was quantified by measuring myeloperoxidase (MPO; Abcam, Cambridge, UK) activity (expressed as U/g) after adding O-dianisidine dihydrochloride and hydrogen peroxide [20 (link)].

Park J.M., Shin J.H., Yang S.W., Lee J.Y., Lee C.L., Lim J.S., Song K.H., Kim G.H, & Na Y.G. (2021). Metformin and Sildenafil Attenuate Inflammation and Suppress Apoptosis After Ischemia/Reperfusion Injuries in Rat Urinary Bladder. International Neurourology Journal, 25(4), 285-295.

+ Open protocol

+ Expand

Colorectal Cancer Therapeutic Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

Distearoylphosphatidylcholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphatiylethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-PEG₂₀₀₀) and cholesterol (Chol), doxorubicin, azoxymethane (AOM), and diallyl trisulfide (DATS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The adenosine deaminase (ADA), lactate dehydrogenase (LDH), superoxide dismutase (SOD) assay, catalase (CAT) assay, lipid peroxidation (MDA) assay, glutathione peroxidase 1 (GPx1) assay, gamma glutamyl transferase (GGT), 5′-nucleotidase (CD73) activity assay and cell cytotoxicity assay (CAA) kit, were procured from Abcam (Cambridge, MA, USA). Dulbecco’s modified Eagle medium (DMEM) was purchased from Santa Cruz Biotechnology Inc, Heidelberg, Germany. Foetal bovine serum (FBS), streptomycin/penicillin, amphotericin-B were procured from Life Technologies, USA. HT-29 (ATCC HTB-38) and RKO (ATCC CRL-2577) were commercially purchased ATCC (American Type Culture Collection), Manassas, VA, USA.

Alrumaihi F., Khan M.A., Babiker A.Y., Alsaweed M., Azam F., Allemailem K.S., Almatroudi A.A., Ahamad S.R., Alsugoor M.H., Alharbi K.N., Almansour N.M, & Khan A. (2022). Lipid-Based Nanoparticle Formulation of Diallyl Trisulfide Chemosensitizes the Growth Inhibitory Activity of Doxorubicin in Colorectal Cancer Model: A Novel In Vitro, In Vivo and In Silico Analysis. Molecules, 27(7), 2192.

+ Open protocol

+ Expand

Streptozotocin-Induced Diabetic Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

Streptozotocin (STZ) and curcumin were purchased from Sigma-Aldrich Inc., St. Louis, MO, USA. The antioxidant enzyme kits including catalase, glutathione, and superoxide dismutase were acquired from Abcam, Cambridge, UK. The inflammatory markers kits for TNF-α, IL-6 and IL-1β were also obtained from Abcam, UK. Antioxidant enzyme and myeloperoxidase kits were purchased from Abcam, UK. For fibrosis evaluation, trichrome stain and a Sirius red kit were purchased from the same company. IL-6 and TNF-α monoclonal antibodies and the Specific HRP/DAB Detection IHC kit were acquired from Abcam, United Kingdom. All supportive chemicals used in this study were of analytical grade and purchased from a local vendor.

Alsulaim A.K., Almutaz T.H., Albati A.A, & Rahmani A.H. (2023). Therapeutic Potential of Curcumin, a Bioactive Compound of Turmeric, in Prevention of Streptozotocin-Induced Diabetes through the Modulation of Oxidative Stress and Inflammation. Molecules, 29(1), 128.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!