Ff01 446 510 581 703 25

Manufactured by IDEX Corporation

The FF01-446/510/581/703-25 is a multipurpose lab equipment product from IDEX Corporation. It is designed to serve as a filter, capable of filtering a range of wavelengths from 446 to 703 nanometers. The product specifications and technical details are available upon request.

Automatically generated - may contain errors

Lab products found in correlation

Lf 405 488 561 635 a 000 zhe, by IDEX Corporation (3 mentions) Cfi apo tirf 100 1.49 na objective, by Nikon (1 mentions) Ilas2 double laser illuminator, by Roper Technologies (1 mentions) Atto647n nhs ester, by ATTO-TEC (1 mentions) Fluorescent microspheres, by Thermo Fisher Scientific (1 mentions) Zen 2012 sp2 black software, by Zeiss (1 mentions) Oyster550, by Synaptic Systems (1 mentions)

3 protocols using ff01 446 510 581 703 25

Live-cell Oblique Illumination Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

For live-cell oblique illumination, neurons were visualized at 50-Hz (10,000–20,000 frames by image streaming) and 20-ms exposure, at 37°C on a microscope equipped with an ILas² double-laser illuminator (Roper Technologies), a CFI Apo TIRF 100× 1.49 NA objective (Nikon), and an Evolve512 delta EMCCD camera (Photometrics). Image acquisition was performed using Metamorph software (MetaMorph Microscopy Automation and Image Analysis Software, version 7.7.8; Molecular Devices). A quadruple beam splitter (LF 405/488/561/635-A-000-ZHE; Semrock) and a QUAD band emitter (FF01-446/510/581/703-25; Semrock) were used.

Joensuu M., Padmanabhan P., Durisic N., Bademosi A.T., Cooper-Williams E., Morrow I.C., Harper C.B., Jung W., Parton R.G., Goodhill G.J., Papadopulos A, & Meunier F.A. (2016). Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles. The Journal of Cell Biology, 215(2), 277-292.

+ Open protocol

+ Expand

Syntaxin-1A Dynamics by uPAINT

Check if the same lab product or an alternative is used in the 5 most similar protocols

uPAINT experiments were performed as per Giannone et al. (2010) (link). To track syntaxin-1A-GFP, we used a GFP nanobody (Kubala et al., 2010 (link)) conjugated to ATTO 647N-NHS-ester (Atto-Tec). DKD-PC12 cells were transfected with syntaxin-1A-GFP alone or cotransfected with either Munc18-1-mCherry or Munc18-1^Δ317-333-mCherry. ATTO 647N–coupled anti-GFP nanobodies were added at a low concentration for stochastic labeling. Time-lapse TIRF videos were captured at 50 Hz (16,000 frames by image streaming) at 37°C. Separate cells were imaged in control conditions or stimulated (imaging was initiated upon addition of 2 mM BaCl₂). We used a quadruple beam splitter (LF 405/488/561/635-A-000-ZHE; Semrock) and a quad band emitter (FF01-446/510/581/703-25; Semrock). The power of the 635-nm laser used was 75–80% of initial laser power (200 mW).

Kasula R., Chai Y.J., Bademosi A.T., Harper C.B., Gormal R.S., Morrow I.C., Hosy E., Collins B.M., Choquet D., Papadopulos A, & Meunier F.A. (2016). The Munc18-1 domain 3a hinge-loop controls syntaxin-1A nanodomain assembly and engagement with the SNARE complex during secretory vesicle priming. The Journal of Cell Biology, 214(7), 847-858.

+ Open protocol

+ Expand

STORM Imaging of Synaptic GABAARs

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were performed on an Elyra PS1 STORM/SIM microscope (Carl Zeiss Microscopy GmbH) equipped with a 100× objective (α Plan-Apochromat 100× 1.46 NA oil-immersion), a focus lock system and an EMCCD camera Andor iXon Ultra 897 (Andor Technologies). A LF488/561-A-000 beam splitter and a FF01–523/610–25 emission filter (Semrock) were used to record SEpH and mEos2 fluorescence. Antibodies against GFP labeled with Alexa 647 and anti-synaptotagmin antibodies labeled with Oyster 550 or Oyster 650 (Synaptic Systems) were detected using LF 405/488/561/635-A-000-ZHE and FF01-446/510/581/703-25 (Semrock). In dual color experiments, red and green channels were aligned with fluorescent microspheres (Molecular Probes) and the channel alignment module in Zen 2012 SP2 (black) software (Carl Zeiss Microscopy GmbH). We collected movies of labeled synaptotagmin sites in live neurons and only bright immobile fluorescent puncta were used to determine presynaptic terminals. Synaptic GABA_ARs were then identified from the overlap of synaptotagmin-rich presynaptic terminals with GFP-labeled γ subunit clusters. Cells were imaged in total internal reflection (TIRF) or highly inclined illumination mode in an enclosed chamber at RT, (32.0 ± 1.5)°C or (36.0 ± 1.5)°C.

Dixon C.L., Sah P., Keramidas A., Lynch J.W, & Durisic N. (2017). γ1-Containing GABA-A Receptors Cluster at Synapses Where they Mediate Slower Synaptic Currents than γ2-Containing GABA-A Receptors. Frontiers in Molecular Neuroscience, 10, 178.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!