Ethanol hplc degree

Ethanol (HPLC degree) and aseptic acid (HPLC degree) were obtained from Merck Co. (Darmstadt, Germany) and methanol (PA) from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). A cellulose ester membrane filter of 0.45 μm (SLCR025NS, Millipore® Co., Bedford, Massachusetts, USA) was used. The carbon dioxide (CO₂) used in the extraction had 99.9% purity (White Martins Gases Industrials–São Paulo, Brazil). The standard 3,5-diprenil-4-hidroxicinamic (Artepillin C–cas number 72944-19-5) was acquired from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and the Acid 4-hidroxicinamic (p-coumaric acid–cas number 501-98-4), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Acid Gallic (cas number 149-91-7), Quercetin (cas number 117-39-5), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (cas number 30931-67-0) and (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (cas number 53188-07-1) were acquired from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).

Ethanol (HPLC degree) and acetic acid (HPLC degree) were obtained from Merck Co. (Darmstadt, Germany) and methanol (PA) from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). A cellulose ester membrane filter of 0.45 μm (SLCR025NS, Millipore Co., Bedford, Massachusetts, USA) was used. The carbon dioxide (CO₂) used in the extraction had 99.9% purity (White Martins Industrials Gases–São Paulo, Brazil). The standard 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C–cas number 72944-19-5) was acquired from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and the acid 4-hydroxycinnamic (p-coumaric acid–cas number 501-98-4), 1,1-diphenil-2-picrilhidrazil (DPPH), Acid Gallic (cas number 149-91-7) and Quercetin (cas number 117-39-5) were acquired from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).