Mercury chloride hgcl2

Klenow fragment (KF-) polymerase (without 3′ to 5′ exonuclease activity) and 10x New England Biolabs (NEB) buffer 2 (100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl₂, 10 mM DTT, pH = 7.9) were obtained from New England Biolabs (Beverly, MA, USA). DNA probe (5'-FAM-CCCCCCCCCGTT CTTCCCTTGTTCG-3') was synthesized by Sangon Biotechnology Co. Ltd. (Shanghai, China). dNTP mixture and TE buffer were purchased from Sangon Biological Engineering Technology and Services Co., Ltd. (Shanghai, China). The Mercury chloride (HgCl₂) and silver nitrate (AgNO₃) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The potassium chloride (KCl), lithium nitrate (LiNO₃), calcium(II) nitrate tetrahydrate (Ca(NO₃)₂∙4H₂O), copper(II) nitrate trihydrate [CuNO₃)₂∙3H₂O], manganese(II) nitrate tetrahydrate [Mn(NO₃) 4H₂O], iron(III) nitrate nonahydrate (Fe(NO₃)₃∙9H₂O), zinc chloride (ZnCl₂) and iron(II) chloride tetrahydrate (FeCl₂∙4H₂O) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Ultra-pure water (18 MΩ∙cm^-1, Kertone Ltd., Changsha, China) was used during all the experiments. The DNA probe was dissolved in TE buffer to 100 μM and stored at −20 °C for further use.

Dulbecco’s modified Eagle’s medium (DMEM), Hank’s Balanced Salt Solution (HBSS) penicillin, streptomycin, and fetal bovine serum (FBS) were obtained from Gibco-Invitrogen (Carlsbad, CA, USA). 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltertrazolium bromide (MTT), 2,3-Dimercapto-1-propanesulfonic acid (DMPS), dimethyl sulfoxide (DMSO), glutathione (GSH), and mercury chloride (HgCl₂) were obtained from Sigma Chemical (St. Louis, MO, USA). Cell scrapper, potassium permanganate, potassium persulfate, trace metal grade (TMG) nitric acid, TMG sulfuric acid, and FITC Annexin V/Dead Cell Apoptosis Kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Tissue culture flasks and 48-well tissue culture plates were purchased from Corning (Corning, NY, USA). Methylmercury chloride stock solution (1,000 ppm) was obtained from Alfa Aesar (Haverhill, MA, USA).