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Anti atp1a1

Manufactured by Abcam
Sourced in Canada, United Kingdom

Anti-ATP1A1 is an antibody product that specifically binds to the ATP1A1 protein. ATP1A1 is the alpha-1 subunit of the sodium/potassium-transporting ATPase, which is responsible for maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane.

Automatically generated - may contain errors

2 protocols using anti atp1a1

1

Immunoblotting Analysis of Prion Proteins

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Immunoblotting analyses of PrP relied on the monoclonal 3F4 antibody (catalog number MAB1562, Millipore Sigma) which recognizes the 109–112 amino acid epitope of human PrP. Antibodies against the NKA subunits consisted of anti-ATP1A1 (catalog number ab7671, Abcam Inc., Toronto, ON, Canada), anti-ATP1A2 (catalog number ab9094-I, Abcam Inc.), anti-ATP1A3 (catalog number MA3-915, Thermo Fisher Scientific) and anti-ATP1B1 (catalog number GTX113390, GeneTex, Irvine, CA, USA). Neuronal and astrocytic markers were detected with the anti-NeuN antibody (catalog number EPR12763, Abcam Inc.), the anti-Tuj-1 antibody (catalog number ab78078, Abcam Inc.) and the anti-GFAP monoclonal (131-17719) antibody (catalog number A-21282, Thermo Fisher Scientific).
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2

Western Blot Analysis of Key Proteins

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Western blot (WB) was performed as described previously.40 Primary antibodies used included anti‐ATP1A1 (Abcam), ATP1B1 (EMD Millipore), E‐cadherin (BD Biosciences), ZO‐1 (Invitrogen, UK), PEDF and GAPDH (Santa Cruz, USA). The immunoblots were detected by Clarity Western ECL Substrate (Bio‐Rad). Cell lysates were collected using RIPA buffer for further WB experiments.
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