Anti atp1a1

Immunoblotting analyses of PrP relied on the monoclonal 3F4 antibody (catalog number MAB1562, Millipore Sigma) which recognizes the 109–112 amino acid epitope of human PrP. Antibodies against the NKA subunits consisted of anti-ATP1A1 (catalog number ab7671, Abcam Inc., Toronto, ON, Canada), anti-ATP1A2 (catalog number ab9094-I, Abcam Inc.), anti-ATP1A3 (catalog number MA3-915, Thermo Fisher Scientific) and anti-ATP1B1 (catalog number GTX113390, GeneTex, Irvine, CA, USA). Neuronal and astrocytic markers were detected with the anti-NeuN antibody (catalog number EPR12763, Abcam Inc.), the anti-Tuj-1 antibody (catalog number ab78078, Abcam Inc.) and the anti-GFAP monoclonal (131-17719) antibody (catalog number A-21282, Thermo Fisher Scientific).

Western blot (WB) was performed as described previously.40 Primary antibodies used included anti‐ATP1A1 (Abcam), ATP1B1 (EMD Millipore), E‐cadherin (BD Biosciences), ZO‐1 (Invitrogen, UK), PEDF and GAPDH (Santa Cruz, USA). The immunoblots were detected by Clarity Western ECL Substrate (Bio‐Rad). Cell lysates were collected using RIPA buffer for further WB experiments.