Db 5ms 5 phenyl methylpolysiloxane column

GC-MS was performed on acid/methanol extracts using a 7890A Series chromatograph coupled to a 5975C Inert XL mass-selective detector (MSD) with a quadrupole mass analyzer (Agilent Technologies, Cheadle, UK). Helium was the carrier gas used with a constant inlet/column head-pressure. A splitless injector was used and maintained at 300 °C and the GC column was inserted directly into the ion source of the mass spectrometer. Spectra were obtained by scanning between 50 and 800 m/z with the ionization energy of the mass spectrometer at 70 eV. Two different column phases were used. General screening was performed using a DB-5ms (5%-phenyl)-methylpolysiloxane column (30 m × 0.250 mm × 0.25 μm; J&W Scientific, Folsom, CA, USA). The temperature for this column was set at 50 °C for 2 min, then raised by 10 °C/min to 325 °C, where it was held for 15 min. TLEs were analyzed with a HT-DB1 GC-MS-FID, 100% dimethylpolysiloxane (15 m × 0.320 mm × 0.1 µm) (J&W Scientific, Folsom, CA, USA) column. The injector was maintained at 350 °C. The temperature of the oven was set at 50 °C for 2 min, and then raised by 10 °C/min to 350 °C, where it was held for 15 min. The column flow was split 9:1 (MSD: Flame ionization detector), with the MSD conditions described above.

GC-MS was performed on both AE and TLE using a 7890A Series chromatograph attached to a 5975C Inert XL mass-selective detector with a quadrupole mass analyser (Agilent Technologies, Cheadle, UK). The carrier gas used was helium, and the inlet/column head-pressure was constant. A splitless injector was used and maintained at 300 °C. The GC column was inserted directly into the ion source of the mass spectrometer. The ionisation energy of the mass spectrometer was 70 eV and spectra were obtained by scanning between m/z 50 and 800. Two different column phases were used. General screening of both AE and TLE was performed using a DB-5 ms (5%-phenyl)-methylpolysiloxane column (30 m × 0.250 mm × 0.25 μm; J&W Scientific, Folsom, CA, USA). The temperature for this column was set at 50 °C for 2 min, then raised by 10 °C/min to 325 °C, where it was held for 15 min. The TLE was also analyzed with a HT-DB1, 100% Dimethylpolysiloxane (15 m × 0.320 mm × 0.1 µm) (J&W Scientific, Folsom, CA, USA). The injector was maintained at 350 °C. The temperature of the oven was set at 50 °C for 2 min, and then raised by 10 °C min⁻¹ to 350 °C, where it was held for 15 min.