Ly6g efluor700

Manufactured by BD

Ly6G-efluor700 is a fluorochrome-conjugated monoclonal antibody that binds to the Ly6G antigen, which is expressed on the surface of neutrophils. The efluor700 fluorescent label allows for the detection and analysis of Ly6G-positive cells using flow cytometry.

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Lab products found in correlation

Siglecf pe, by BD (2 mentions) F4 80 apc, by BD (2 mentions) Cd11c fitc, by BD (2 mentions) Rat anti mouse cd3 cd19 cf594, by BD (2 mentions) Cd301 pecy7, by BD (2 mentions) Cd64 percp cy5, by BD (2 mentions) Cd11b bv650, by BD (2 mentions) Facsaria, by BD (2 mentions) Facsdiva software, by BD (1 mentions)

2 protocols using ly6g efluor700

Comprehensive Immune Cell Profiling

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Cell surface marker analysis was performed using flow cytometry. Single-cell suspensions prepared from MLN, adipose tissue (AT) and liver were collected from mice at the times indicated. Cell surface markers were stained for 30 min at 4°C with rat anti-mouse CD3/CD19-CF594 (Clone:145-2C11,1D3) F4/80-APC (Clone: T45-2342), CD11c-FITC (Clone: HL3), CD301-pecy7 (Clone: LOM-14), CD64-PerCp-Cy5.5 (Clone: X45-5/7.1), CD11b-BV650 (Clone: M1/70), Ly6G-efluor700 (Clone: 1A8) and Siglec-F-PE (Clone: E50-2440) (BD Bioscience). All antibody incubations were performed at 4°C for 30 min (isotype controls were included). Data were acquired using a BD FACS Aria and analyzed using FlowJo software (Tree Star, Inc).

Khudhair Z., Alhallaf R., Eichenberger R.M., Whan J., Kupz A., Field M., Krause L., Wilson D.T., Daly N.L., Giacomin P., Sotillo J, & Loukas A. (2021). Gastrointestinal Helminth Infection Improves Insulin Sensitivity, Decreases Systemic Inflammation, and Alters the Composition of Gut Microbiota in Distinct Mouse Models of Type 2 Diabetes. Frontiers in Endocrinology, 11, 606530.

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Multiparametric Flow Cytometry Analysis

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Cell surface markers were stained for 30 min at 4 °C with rat anti-mouse CD3/CD19-CF594 (Clone:145-2C11,1D3) F4/80-APC (Clone: T45-2342), CD11c-FITC (Clone: HL3), CD301-pecy7 (Clone: LOM-14), CD64-PerCp-Cy5.5 (Clone: X45-5/7.1), CD11b-BV650 (Clone: M1/70), Ly6G-efluor700 (Clone: 1A8), and Siglec-F-PE (Clone: E50-2440) (BD Bioscience). All antibody incubations were performed at 4 °C for 30 min (isotype controls were included). Data were acquired using a BD FACS Aria with BD FACS DIVA software (BD Bioscience) and analysed using FlowJo software (Tree Star, Inc., Ashland, OR, USA).

Khudhair Z., Alhallaf R., Eichenberger R.M., Field M., Krause L., Sotillo J, & Loukas A. (2022). Administration of Hookworm Excretory/Secretory Proteins Improves Glucose Tolerance in a Mouse Model of Type 2 Diabetes. Biomolecules, 12(5), 637.

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