The proteins were separated from frozen renal tissues and the protein concentration was measured by bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific, Rockford, IL, USA). The protein samples were resolved and transferred onto polyvinylidene fluoride (PVDF) membranes. After blocking with 5% nonfat milk at room temperature for 2 h, the membranes were performed using specific primary antibody as follows: haptoglobin and alpha-1-antitrypsin (Abcam, Cambridge, MA, USA). The blots were incubated with horseradish peroxidase-conjugated secondary antibodies (Abcam, Cambridge, MA, USA) and then exposed with an ECL kit (GE Healthcare, Chicago, IL, USA).
Alpha 1 antitrypsin
Manufactured by Abcam
Sourced in United States
Alpha-1-antitrypsin is a laboratory reagent used in various research and diagnostic applications. It is a serine protease inhibitor that plays a crucial role in regulating proteolytic activity in the body. This product is suitable for use in techniques such as western blotting, ELISA, and other related assays.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated secondary antibody, by Abcam (1 mentions)
Haptoglobin, by Abcam (1 mentions)
Ecl kit, by GE Healthcare (1 mentions)
Bca assay kit, by Thermo Fisher Scientific (1 mentions)
Il 12p40, by R&D Systems (1 mentions)
Il 12p70, by R&D Systems (1 mentions)
Citrullinated histone h3, by Cayman Chemical (1 mentions)
Vegf a, by R&D Systems (1 mentions)
Mmp 1, by R&D Systems (1 mentions)
2 protocols using alpha 1 antitrypsin
1
Protein Quantification and Immunoblotting
2
Multiplex Biomarker Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Luminex, model L200 (Millipore, Massachusetts, USA) was used to evaluate serum levels of, IL-8, IL-12p40, IL-12p70, VEGF-A, MMP-1, and-8 (R&D Systems, Minneapolis-USA). The results were presented in median fluorescence intensity (MFI) units.
Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum levels of citrullinated histone H3 (Cayman, Michigan-USA) and alpha-1-antitrypsin (Abcam, Cambridge-USA).
Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum levels of citrullinated histone H3 (Cayman, Michigan-USA) and alpha-1-antitrypsin (Abcam, Cambridge-USA).
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