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Psma 1h8h5

Manufactured by Thermo Fisher Scientific
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Sourced in United States

PSMA (1H8H5) is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed for specific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information about the core function and intended use of this product is not available.

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Lab products found in correlation

Tubulin 9f3, by Cell Signaling Technology (2 mentions) Gapdh gt239, by GeneTex (2 mentions) Mglur1, by Merck Group (2 mentions) Calnexin c5c9, by Cell Signaling Technology (2 mentions) Cellytic m lysis buffer, by Merck Group (2 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Phosstop, by Roche (2 mentions) Pvdf membrane, by Merck Group (1 mentions)

2 protocols using psma 1h8h5

1

Western Blot Protein Analysis Protocol

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Cells were washed with ice-cold PBS, harvested by scraping and then homogenized in CelLytic M lysis buffer (Sigma, Merck) supplemented with phosphatase and protease inhibitors (PhosSTOP and cOmplete, Roche). Lysates were incubated (1 hour, 4°C) and then centrifuged at 14,000 × g (10 minutes, 4°C). Protein samples were quantified using the Pierce BCA Protein Assay (Thermo Fisher Scientific), resolved by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Merck). Membranes were blocked with Tris-buffered saline/0.5% Tween 20 (TBST, pH 7.5) containing 5% skim milk powder and probed overnight with primary antibodies against the following targets: PSMA (1H8H5, Thermo Fisher Scientific), EGFR (D38B1), HER2 (44E7), HER3 (D22C5), p-AKT(S473) (587F11), p-AKT (T308) (244F9), AKT (D38B1), p-S6 (S235/236) (D57.2.2E), Calnexin (C5C9) and Tubulin (9F3) were from Cell Signaling Technology, AR (Santa Cruz Biotechnology), GAPDH (GT239, GeneTex), and mGluR1 (07-617, Merck). Following incubation with horseradish peroxidase–linked secondary antibodies (Dako, Agilent Technologies), immunoreactive bands were visualized with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific).
Gómez V., Galazi M., Weitsman G., Monypenny J., Al-Salemee F., Barber P.R., Ng K., Beatson R., Szokol B., Orfi L., Mullen G., Vanhaesebroeck B., Chowdhury S., Leung H.Y, & Ng T. (2022). HER2 Mediates PSMA/mGluR1-Driven Resistance to the DS-7423 Dual PI3K/mTOR Inhibitor in PTEN Wild-type Prostate Cancer Models. Molecular Cancer Therapeutics, 21(4), 667-676.
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2

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with ice-cold PBS, harvested by scraping and then homogenized in CelLytic M lysis buffer (Sigma, Merck) supplemented with phosphatase and protease inhibitors (PhosSTOP and cOmplete, Roche, Basel, Switzerland). Lysates were incubated (1 h, 4°C) and then centrifuged at 14,000 x g (10 min, 4°C). Protein samples were quantified using the Pierce BCA Protein Assay (ThermoFisher Scientific), resolved by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck). Membranes were blocked with Tris-buffered saline/0.5% Tween 20 (TBST, pH 7.5) containing 5% skim milk powder and probed overnight with primary antibodies against the following targets: PSMA (1H8H5, ThermoFisher Scientific), EGFR (D38B1), HER2 (44E7), HER3 (D22C5), p-AKT(S473) (587F11), p-AKT (T308) (244F9), AKT (D38B1), p-S6 (S235/236) (D57.2.2E), Calnexin (C5C9) and Tubulin (9F3) were from Cell Signaling Technology, MA, USA, AR (Santa Cruz Biotechnology, TX, USA), GAPDH (GT239, GeneTex, CA, USA) and mGluR1 (07-617, Merck). Following incubation with horseradish peroxidase-linked secondary antibodies (Dako, Agilent Technologies, CA, USA), immunoreactive bands were visualized with Pierce ECL Western Blotting Substrate (ThermoFisher Scientific).
Gómez V., Galazi M., Weitsman G., Monypenny J., Al-Salemee F., Barber P.R., Ng K., Beatson R., Szokol B., Orfi L., Mullen G., Vanhaesebroeck B., Chowdhury S., Leung H.Y, & Ng T. (2022). HER2 Mediates PSMA/mGluR1-Driven Resistance to the DS-7423 Dual PI3K/mTOR Inhibitor in PTEN Wild-type Prostate Cancer Models. Molecular Cancer Therapeutics, 21(4), 667-676.
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