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Cck ires cre mice

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Cck-IRES-Cre mice are a genetically modified mouse line that expresses the Cre recombinase enzyme under the control of the cholecystokinin (Cck) gene promoter. The Cre recombinase enzyme can be used to induce targeted gene modifications in cells that express the Cck gene.

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Lab products found in correlation

Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Rnascope multiplex fluorescent assay, by Advanced Cell Diagnostics (1 mentions) Dm5500 fluorescent microscope, by Leica (1 mentions) Sf1 cre, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Ai14 mice, by Jackson ImmunoResearch (1 mentions) Camkiia cre mice, by Jackson ImmunoResearch (1 mentions) Clozapine, by Merck Group (1 mentions)

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CCK-ires-Cre (8 citations) CCK-Cre (7 citations)

5 protocols using cck ires cre mice

1

Behavioral Optogenetics in Mice

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Cck-IRES-Cre mice (Jackson Laboratory stock No. 012706) and wild-type C57BL/6 J mice (Jackson Laboratory stock No. 000664) were used for all experiments. Male and female mice between 2 and 6 months of age were used in all experiments. Mice were maintained on a 12 hr reverse light–dark cycle with food and water ad libitum. Sample sizes were chosen based on previous behavioral optogenetics studies on defensive behaviors, which typically use 6–15 mice per group. All mice were handled for a minimum of 5 days prior to any behavioral task.
Wang W., Schuette P.J., La-Vu M.Q., Torossian A., Tobias B.C., Ceko M., Kragel P.A., Reis F.M., Ji S., Sehgal M., Maesta-Pereira S., Chakerian M., Silva A.J., Canteras N.S., Wager T., Kao J.C, & Adhikari A. (2021). Dorsal premammillary projection to periaqueductal gray controls escape vigor from innate and conditioned threats. eLife, 10, e69178.
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2

RNA Localization in Cck-IRES-Cre Mouse Brains

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Cck-IRES-Cre mice (Jackson Laboratory stock No. 012706) were euthanized with 5% isoflurane followed by cervical dislocation. Brains were harvested and snap-frozen in 2-methylbutane at −20°C and tissue was sectioned at 18 μm. The workflow was performed in accordance with the manufacturer’s protocol for the RNAScope® Multiplex Fluorescent Assay (Advanced Cell Diagnostics, Newark, CA). Riboprobes selective for sequences were labeled as follows: Cre (C1, Cat. No. 312281), nNOS with (437651-C2) and NeuN (Mm-Rbfox3-C3, Cat No. 313311-C3) or VGLUT2 (Mm-Slc17a6-C3, Cat No. 319171-C3). Images were obtained with a Leica DM5500 fluorescent microscope at 40x.
Wang W., Schuette P.J., Nagai J., Tobias B.C., Reis F.M., Ji S., de Lima M.A., La-Vu M.Q., Maesta-Pereira S., Chakerian M., Leonard S.J., Lin L., Severino A.L., Cahill C.M., Canteras N.S., Khakh B.S., Kao J.C, & Adhikari A. (2021). Coordination of escape and spatial navigation circuits orchestrate versatile flight from threats. Neuron, 109(11), 1848-1860.e8.
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3

Optogenetic Manipulation of Defensive Behaviors

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Cck-IRES-Cre mice (Jackson Laboratory stock No. 012706), SF1-cre (Jackson Laboratory stock No. 012462) and wild type C57BL/6J mice (Jackson Laboratory stock No. 000664) were used for all experiments. Male and female mice between 2 and 6 months of age were used in all experiments. Mice were maintained on a 12-hour reverse light-dark cycle with food and water ad libitum. Sample sizes were chosen based on previous behavioral optogenetics studies on defensive behaviors, which typically use 6–15 mice per group. All mice were handled for a minimum of 5 days prior to any behavioral task.
Wang W., Schuette P.J., Nagai J., Tobias B.C., Reis F.M., Ji S., de Lima M.A., La-Vu M.Q., Maesta-Pereira S., Chakerian M., Leonard S.J., Lin L., Severino A.L., Cahill C.M., Canteras N.S., Khakh B.S., Kao J.C, & Adhikari A. (2021). Coordination of escape and spatial navigation circuits orchestrate versatile flight from threats. Neuron, 109(11), 1848-1860.e8.
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4

Neurobehavioral Experiments in Mice and Rats

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In this study, we utilized adult C57BL/6J mice, CaMKIIa-Cre mice (Jackson lab stock #005359), Ai14 mice (Jackson lab stock #007914), CckIres-Cre mice (Jackson lab stock #012706), and Sprague–Dawley rats. For the behavioral experiments, we exclusively used male subjects. All animals were verified to have clean ears and normal hearing. They were kept under a controlled environment with a 12 hr-light/12 hr dark cycle, with the light period spanning from 8:00 pm to 8:00 am the following day. The temperature was maintained at 20–24°C and humidity levels were kept between 40 and 60%. All animals were provided with unlimited access to both food and water. All experimental procedures received approval from the Animal Subjects Ethics Sub-Committee of the City University of Hong Kong (reference number of animal ethics review: A-59 and A-0467).
Sun W., Wu H., Peng Y., Zheng X., Li J., Zeng D., Tang P., Zhao M., Feng H., Li H., Liang Y., Su J., Chen X., Hökfelt T, & He J. (2024). Heterosynaptic plasticity of the visuo-auditory projection requires cholecystokinin released from entorhinal cortex afferents. eLife, 13, e83356.
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5

Chemogenetic Dissection of CCK-Positive Circuits

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A chemogenetics experiment was performed on Cck-ires-Cre mice (#012706, Jackson Laboratories). A Cre-dependent hM4Di virus was injected into the rhinal cortex. Detailed coordinates and volumes were described in the virus injection part. Besides, to specifically inhibit CCK-positive projections from the RC to the MC, a retro-Cre virus (AAV(retro)-EF1a-Cre-EGFP) was injected into the MC bilaterally and a Cre-dependent hM4Di virus (AAV-hSyn-DIO-hM4Di-mCherry) was injected into the RC of C57BL/6 mice. Mice were used for single pellet reaching task training 4 weeks post virus injection. Thirty minutes before behavior training, clozapine (0.4 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) was intraperitoneally injected to inactivate the activity of the CCK-positive neurons in the rhinal cortex. The same volume of vehicle (0.9% saline solution with 0.1% DMSO) was injected into mice injected with Cre-dependent hM4Di as a sham control group. A negative control virus (AAV8-hSyn-DIO-mCherry) combined with intraperitoneal clozapine injection was also carried out to exclude the influence of clozapine on motor learning ability.
Li H., Feng J., Chen M., Xin M., Chen X., Liu W., Wang L., Wang K.H, & He J. (2024). Cholecystokinin facilitates motor skill learning by modulating neuroplasticity in the motor cortex. eLife, 13, e83897.
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