Optical rotations were recorded with a 343 Plus polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a V-530 spectrophotometer (Jasco, Tokyo, Japan). IR spectra were obtained on a Jasco FT/IR 300-E spectrometer, and CD spectra were recorded on a Jasco J-815 CD spectrometer. NMR experiments were recorded using an Avance III 400 spectrometer (Bruker, Billerica, MA, USA) with TMS as the internal standard. HRESIMS were determined on Waters Synapt G2 QTOF (Waters, Milford, MA, USA). TLC was carried out on Merck silica gel F254-precoated glass plates and RP-18 F254s plates. Chromatography was performed on a Waters 1525 Binary HPLC pump connected to a 996 Photodiode Array (PDA) detector using Isco Allsphere ODS-2 (10 μm, 10 × 250 mm) and Nova-Pak C18 (4 μm, 3.9 × 150 mm) columns.
343 plus polarimeter
Manufactured by PerkinElmer
Sourced in United States
The 343 Plus polarimeter is a laboratory instrument used to measure the optical rotation of samples. It is designed to provide accurate and reliable polarimetric measurements across a wide range of applications.
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Lab products found in correlation
V 530 spectrophotometer, by Jasco (3 mentions)
Ft ir 300 e spectrometer, by Jasco (2 mentions)
Avance 3 hd 400 spectrometer, by Bruker (2 mentions)
Unity inova 500 spectrometer, by Agilent Technologies (2 mentions)
Avance 3 400 spectrometer, by Bruker (1 mentions)
Waters synapt g2 qtof, by Waters Corporation (1 mentions)
J 815 cd spectrometer, by Jasco (1 mentions)
1600 spectrophotometer, by PerkinElmer (1 mentions)
Xevo g2 s qtof instrument, by Waters Corporation (1 mentions)
Avance 400 mhz, by Bruker (1 mentions)
Synapt g2 mass spectrometer, by Waters Corporation (1 mentions)
996 photodiode array detector, by Waters Corporation (1 mentions)
Silica gel 60 f254 glass plates, by Merck Group (1 mentions)
Waters 600e multi solvent delivery system, by Waters Corporation (1 mentions)
1525 binary hplc pump, by Phenomenex (1 mentions)
Jms t200gc accutof gcx plus high performance gas chromatograph time of flight mass spectrometer, by JEOL (1 mentions)
4 protocols using 343 plus polarimeter
1
Characterization of Chemical Compounds
2
Extraction and Characterization of Crotalaria cleomifolia Seed Compounds
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Pods of Crotalaria cleomifolia were harvested in May 2014 at Manjakandriana, near Antananarivo (geographical coordinates, south: 18°54′06.9′′, East: 047°45′08.1′′). Seeds were separated from pods then dried at room temperature in the shadow. After drying, they were crushed in a fine powder using an electric grinder. Solvents used for the extraction were purchased from Cooper (Paris, France) and were used as received. Reagents used for the qualitative studies were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
1H and 13C NMR (1D and 2D) spectra were measured on a Bruker Avance 400 MHz (Mannheim, Germany) NMR spectrometer in CDCl3 or CD3OD. Chemical shifts are reported in parts per million (δ) and coupling constants (J) in Hz. The residual CDCl3 or CD3OD were used as internal standards for 1H and 13C NMR, respectively. IR spectra were measured on a Perkin Elmer 1600 spectrophotometer. Optical rotations were measured at room temperature using a Perkin Elmer 343 Plus polarimeter. Electrospray ionization (ESI) mass spectra were recorded at the Institut de Chimie Moléculaire de Grenoble on a Waters Xevo G2-S Q TOF instrument (Guyancourt, France) with a nanospray inlet.
1H and 13C NMR (1D and 2D) spectra were measured on a Bruker Avance 400 MHz (Mannheim, Germany) NMR spectrometer in CDCl3 or CD3OD. Chemical shifts are reported in parts per million (δ) and coupling constants (J) in Hz. The residual CDCl3 or CD3OD were used as internal standards for 1H and 13C NMR, respectively. IR spectra were measured on a Perkin Elmer 1600 spectrophotometer. Optical rotations were measured at room temperature using a Perkin Elmer 343 Plus polarimeter. Electrospray ionization (ESI) mass spectra were recorded at the Institut de Chimie Moléculaire de Grenoble on a Waters Xevo G2-S Q TOF instrument (Guyancourt, France) with a nanospray inlet.
3
Analytical Characterization of Organic Compounds
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Optical rotations were measured with a 343 Plus polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a JASCO V-530 spectrophotometer, and IR spectra were obtained on a JASCO FT/IR-300E spectrometer (Jasco, Tokyo, Japan). NMR experiments were recorded using a Varian Unity Inova 500 spectrometer (Varian, Palo Alto, CA, USA) and a Bruker Avance III HD 400 spectrometer (Bruker, Billerica, MA, USA) with TMS as an internal standard. HRESIMS analysis was performed on a Waters Synapt G2 mass spectrometer (Waters, Milford, MA, USA). TLC was carried out on precoated silica gel 60 F254 glass plates (Merck, Darmstadt, Germany). Visualization of the silica gel TLC was performed using an anisaldehyde-H2SO4 spray reagent. The adsorbent used for column chromatography was silica gel 70–230 mesh. HPLC was performed on a Waters 600E Multisolvent Delivery System (Waters, Milford, MA, USA) connected to a Waters 996 Photodiode Array Detector using Zorbax SB-CN (10 μm, 9.4 × 250 mm) and Chiralpak AD-H (5 μm, 250 × 4.6 mm) columns.
4
Comprehensive Spectroscopic Analysis of Compounds
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UV spectra were taken with a Jasco V-530 spectrophotometer (Jasco, Tokyo, Japan), and IR spectra were obtained on a PerkinElmer Spectrum 400 FT-IR/NIR spectrometer (Waltham, MA, USA). Optical rotations were measured on a PerkinElmer 343 Plus polarimeter (Waltham, MA, USA). HRFDMS were measured on a JMS-T200GC AccuTOF™ GCx-plus High Performance Gas Chromatograph-Time-of-Flight Mass Spectrometer (Jeol Ltd., Tokyo, Japan). NMR experiments were performed on a Varian Unity Inova 500 spectrometer (Varian Inc., Palo Alto, CA, USA) or a Bruker Avance III HD 400 spectrometer (Bruker, Billerica, MA, USA) using DMSO-d6 and CD3OD as solvents and TMS as internal standard. Chemical shift values (δ) were reported in parts per million (ppm), and the coupling constants (J) were reported in hertz (Hz). Thin-layer chromatography (TLC) was carried out on precoated silica gel 60 F254 plates (Merck, Darmstadt, Germany). Reversed-phase HPLC was performed on a Waters 1525 Binary HPLC pump equipped with a 996 photodiode array (PDA) detector using a Phenomenex Luna C18 (250 × 10 mm, 5 μm) column or a Zorbax Rx C8 (150 × 4.6 mm, 5 μm) with MeOH-H2O at a flow rate of 2.0 mL/min or 1.0 mL/min, respectively. Separation of diastereomers was carried out on a YMC Amylose-SA chiral column (250 × 4.6 mm, 5 μm) with hexane-ethanol at a flow rate of 1.0 mL/min.
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