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Anti urgcp

Manufactured by Merck Group
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Sourced in United States

Anti-URGCP is a laboratory equipment product designed for the detection and analysis of the URGCP (Upregulated Gene in Colorectal Cancer) protein. It serves as a tool for researchers and scientists studying the role of URGCP in various biological processes and disease pathways.

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Lab products found in correlation

Anti gapdh, by Merck Group (2 mentions) Anti p65, by Cell Signaling Technology (2 mentions) Anti p84, by Cell Signaling Technology (2 mentions) Anti phosphor iκbα, by Cell Signaling Technology (2 mentions) Cellytic nuclear extraction kit, by Merck Group (1 mentions) Monoclonal anti α tubulin antibody, by Merck Group (1 mentions) Anti ikkβ, by Cell Signaling Technology (1 mentions) Anti iκbα, by Cell Signaling Technology (1 mentions) Anti c jun, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti cdk4, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Anti cyclin e1, by Cell Signaling Technology (1 mentions) Anti β tubulin, by Merck Group (1 mentions) Anti c myc, by Cell Signaling Technology (1 mentions) Anti cdk2, by Merck Group (1 mentions) Anti cyclin a, by Santa Cruz Biotechnology (1 mentions) Anti cdk6, by Cell Signaling Technology (1 mentions)

2 protocols using anti urgcp

1

Western Blot Analysis of NF-κB Pathway

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Western blot was conducted according to a standard method previously described [44 (link)], using anti-URGCP (Sigma, Saint Louis, MI), anti-phosphor-IκBα, anti-IκBα, anti-phosphor-IKKβ, anti-IKKβ, anti-p65, anti-c-Jun or anti-P84 antibodies (Cell signaling, Danvers, MA). Blotted membranes were stripped and re-blotted with an anti-GAPDH or anti-α-tubulin monoclonal antibody (Sigma) as a loading control. The nuclear proteins were extracted using CelLytic NuCLEAR Extraction Kit (Sigma) according to the manufacturer's instructions.
Cai J., Li R., Xu X., Zhang L., Wu S., Yang T., Fang L., Wu J., Zhu X., Li M, & Huang Y. (2015). URGCP promotes non-small cell lung cancer invasiveness by activating the NF-κB-MMP-9 pathway. Oncotarget, 6(34), 36489-36504.
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2

Western Blot Antibody Validation

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The following antibodies were used for Western blotting: anti-URGCP, anti-β-actin, anti-β-tubulin and anti-GAPDH (Sigma), anti-CDK2, anti-CDK4, anti-CDK6, anti-Cyclin D1, anti-Cyclin E1, anti-c-myc, anti-p65, anti-phosphor-IκBα and anti-P84 (Cell signaling, Danvers, MA), anti-Cyclin A (Santa Cruz, CA, USA). Details are described in supplementary methods.
Hong L., Qing O., Ji Z., Chengqu Z., Ying C., Hao C., Minhui X, & Lunshan X. (2017). Downregulation of miR-16 via URGCP pathway contributes to glioma growth. Scientific Reports, 7, 13470.
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