For gene array, corneas were processed using a magnetic bead-based Quantigene 2.0 multiplex kit (Affymetrix). Briefly, samples were lysed to extract RNA and incubated with specific target probes overnight. Signals were amplified with a hybridization technique, and detected using a luminex instrument (Biorad) after adding streptavidin with phycoerythrin as a substrate. For protein array, tissues were homogenized with a Tissuemiser (Fisher Scientific) in Tissue Protein Extraction Reagent (T-PER, Fisher Scientific) in the presence of 1x Calbiochem protease inhibitor cocktail set I (Millipore). Homogenates were centrifuged in a microcentrifuge at 10,000×g for 90 sec at 4° C, and the supernatants were evaluated for analyte content by multiplex (Millipore) or ELISA (R&D) assays.
Quantigene 2.0 multiplex kit
Manufactured by Thermo Fisher Scientific
The Quantigene 2.0 multiplex kit is a laboratory equipment product from Thermo Fisher Scientific. It is designed for the quantitative measurement of multiple gene expression targets simultaneously in a single sample.
Automatically generated - may contain errors
2 protocols using quantigene 2.0 multiplex kit
1
Multiplex Gene and Protein Profiling
2
Multiplex Gene and Protein Profiling
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