To confirm isotype specificity of serum Dsg3-specific antibody responses, ELISA plates were coated with 2 μg/mL of rDsg3. Plates were washed and blocked as previously described. Plasma samples from ISD068 and ISD102 were added to the plates in a serial dilution starting at 1:100 dilutions. After a 90-minute incubation at room temperature, plates were washed and incubated with peroxidase-conjugated mouse anti-human IgG1, IgG2, IgG3, or IgG4 subclass specific reagent (Molecular probe) or a peroxidase-conjugated goat anti-human IgG or IgA reagent (Jackson ImmunoResearch) and incubated for an additional 90 min at room temperature. Plates were washed and IgG subclass signal was amplified by an additional incubation with peroxidase conjugated goat-anti mouse IgG (Kirkegaard & Perry Lab, Inc.) for 90 min at room temperature. Plates were then developed and analyzed as described above.
Peroxidase conjugated goat anti human igg or iga reagent
Manufactured by Jackson ImmunoResearch
Peroxidase-conjugated goat anti-human IgG or IgA reagent is a laboratory product that contains goat-derived antibodies specific to human immunoglobulin G (IgG) or immunoglobulin A (IgA). These antibodies are conjugated to the enzyme peroxidase, which can be used as a detection label in various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peroxidase conjugated goat anti human igg or iga reagent
1
Determination of Dsg3 Antibody Isotypes
2
Determination of Dsg3 Antibody Isotypes
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To confirm isotype specificity of serum Dsg3-specific antibody responses, ELISA plates were coated with 2 μg/mL of rDsg3. Plates were washed and blocked as previously described. Plasma samples from ISD068 and ISD102 were added to the plates in a serial dilution starting at 1:100 dilutions. After a 90-minute incubation at room temperature, plates were washed and incubated with peroxidase-conjugated mouse anti-human IgG1, IgG2, IgG3, or IgG4 subclass specific reagent (Molecular probe) or a peroxidase-conjugated goat anti-human IgG or IgA reagent (Jackson ImmunoResearch) and incubated for an additional 90 min at room temperature. Plates were washed and IgG subclass signal was amplified by an additional incubation with peroxidase conjugated goat-anti mouse IgG (Kirkegaard & Perry Lab, Inc.) for 90 min at room temperature. Plates were then developed and analyzed as described above.
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