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Glycine

Manufactured by Vivantis Technologies
Sourced in Malaysia

Glycine is a colorless, crystalline powder that is used as a chemical reagent in laboratory settings. It is a simple amino acid with the chemical formula C₂H₅NO₂. Glycine serves as a fundamental building block for the synthesis of proteins and other biomolecules. Its core function is to facilitate chemical reactions and analyses in a variety of laboratory applications.

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4 protocols using glycine

1

Cell Culture Reagents and Chemicals

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DMEM, Opti-MEM, geneticin, fetal bovine serum (FBS) and lipofectamine 2000 were from Invitrogen (Carlsbad, CA, USA). Penicillin-streptomycin mix was from PAA. Tris and Glycine were from Vivantis (Selangor Darul Ehsan, Malaysia). PDBu, poly-D-lysine, GI254023X and dimethyl sulfoxide were from Sigma (St Louis, MO, USA). Skim milk powder was from HiMedia (Mumbai, India). Ammonium persulphate was from GE Health care (Pisataway, NJ, USA). The chemiluminescence HRP substrate was from Millipore (Bedford, MA, USA). SDS was from Amaresco (Solon, OH, USA). O-Phenanthroline and TAPI-0 were from Calbiochem (San Diego, CA, USA).
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2

Purification of Plant-Produced Antibody Fragments

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Infiltrated N. benthamiana leaves were extracted with 1X PBS, pH 7.4 using a blender (JSR, Gongju-si, Korea) and centrifuged (Thermo Scientific, Massachusetts, USA) at 26,000 g for 30 min. The supernatant was filtered with a 0.45 µm membrane filter (Pall Corporation, New York, USA) and loaded onto a protein-A affinity column. The column was washed with ten-column volumes of PBS pH 7.4. The bound protein was eluted with 0.1 M glycine (Vivantis, Shah Alam, Malaysia), pH 2.7, and rapidly neutralized to pH 7.4 with 1.5 M Tris–HCl (Vivantis, Shah Alam, Malaysia), pH 8.8. The purified plant-produced VHH-αNTX-Fc and VHH-PLA2-Fc antibody was analyzed using SDS-PAGE and immunoblot analysis.
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3

Carbohydrate-based Analytical Procedures

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Maltooligosaccharide (G1-4), soluble potato starch, corn starch, bovine serum albumin and ampicillin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tryptone and yeast extract were obtained from Difco (Bacton Dickinson and company, Sparks, MD, USA). β-CD was purchased from AppliChem (Darmstadt, Germany). Standard maltitol and D-sorbitol were obtained from TCI (Toshima, Kita-ku, Tokyo, Japan). Aquacide II, methanol, isopropanol, ethyl acetate, acetonitrile, HPLC-grade water, potassium dihydrogen phosphate, dipotassium phosphate and TLC silica gel 60 F254 glass plates (20 cm in height) were purchased from Merck (Darmstadt, Germany). IPTG, glycine and Tris-HCl were obtained from Vivantis (Sendirian Berhad, Shah Alam, Malaysia). Sulfuric acid was obtained from Carlo Erba Reagents (Val de Reuil, France). The commercial glucose oxidase kit was obtained from Human mit-diagnostics GmbH (Idstein, Germany). Other chemicals used were of an analytical grade.
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4

Alhydrogel® Adjuvant Formulation Protocol

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Alhydrogel® adjuvant (2%; catalog: vac-alu-250, CAS number: 21645-51-2) containing an aqueous suspension of aluminum hydroxide gel (alum) was procured from InvivoGen, USA. Vaccine excipients sucrose (catalog: 107651, CAS number: 57-50-1) was purchased from Merck, Germany, and glycine (catalog: PR0608, CAS number: 56-40-6) was acquired from Vivantis Technologies, Malaysia.
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