Glycine

DMEM, Opti-MEM, geneticin, fetal bovine serum (FBS) and lipofectamine 2000 were from Invitrogen (Carlsbad, CA, USA). Penicillin-streptomycin mix was from PAA. Tris and Glycine were from Vivantis (Selangor Darul Ehsan, Malaysia). PDBu, poly-D-lysine, GI254023X and dimethyl sulfoxide were from Sigma (St Louis, MO, USA). Skim milk powder was from HiMedia (Mumbai, India). Ammonium persulphate was from GE Health care (Pisataway, NJ, USA). The chemiluminescence HRP substrate was from Millipore (Bedford, MA, USA). SDS was from Amaresco (Solon, OH, USA). O-Phenanthroline and TAPI-0 were from Calbiochem (San Diego, CA, USA).

Maltooligosaccharide (G_1-4), soluble potato starch, corn starch, bovine serum albumin and ampicillin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tryptone and yeast extract were obtained from Difco (Bacton Dickinson and company, Sparks, MD, USA). β-CD was purchased from AppliChem (Darmstadt, Germany). Standard maltitol and D-sorbitol were obtained from TCI (Toshima, Kita-ku, Tokyo, Japan). Aquacide II, methanol, isopropanol, ethyl acetate, acetonitrile, HPLC-grade water, potassium dihydrogen phosphate, dipotassium phosphate and TLC silica gel 60 F254 glass plates (20 cm in height) were purchased from Merck (Darmstadt, Germany). IPTG, glycine and Tris-HCl were obtained from Vivantis (Sendirian Berhad, Shah Alam, Malaysia). Sulfuric acid was obtained from Carlo Erba Reagents (Val de Reuil, France). The commercial glucose oxidase kit was obtained from Human mit-diagnostics GmbH (Idstein, Germany). Other chemicals used were of an analytical grade.