Glutathione (gsh)

Preparation of 20 mmol·L⁻¹ MT solution: 0.2330 g of MT was precisely weighed, and dissolved with anhydrous ethanol in an ultra-clean bench (Suzhou Jedi Purification Technology Co., Ltd., Suzhou, Jiangsu, China). The solution was slowly fixed with sterile water to 50 mL, and filtered in a sterilized centrifuge tube with a 0.22 μm sterilized filter tip (Shanghai Anpu Experimental Technology Co., Ltd., Shanghai, China). Light was avoided throughout the operation. It was stored at 4 °C in a refrigerator to avoid light, and re-dissolved in a water bath with heat before use if precipitate was produced. MT was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China) with a purity of 99% and CAS number: 73-31-4.
Preparation of 20 mmol·L⁻¹ GSH solution: 0.3073 g of GSH was precisely weighed. The solution was slowly fixed with sterile water to 50 mL, and filtered in a sterilized centrifuge tube with a 0.22 μm sterilized filter tip (Shanghai Anpu Experimental Technology Co., Ltd., Shanghai, China). It was stored at 4 °C in a refrigerator, and re-dissolved in a water bath with heat before use. GSH was purchased from Shanghai Yuanye Biotechnology Co., Ltd. in China with a purity of 99% and CAS number: 70-18-8.

l-NAC (≥99.0%) and GSH (98.0%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd Methanol (≥99.5%), dichloromethane (≥99.5%), ethyl acetate (≥99.5%), and petroleum ether were purchased from Shanghai Titan Scientific Co. Ltd. Hexane (≥97.0%) and trifluoroacetic acid (TFA) were purchased from Sinopharm Chemical Reagent Co., Ltd Methanol (≥99.9%) and acetonitrile (≥99.9%) for high-performance liquid chromatography (HPLC) were purchased from Sigma Aldrich. The purified water used in the experiment was obtained from a purification system with a resistivity of 18 MO cm⁻¹. All other reagents and chemicals were of analytical grade and used without further purification.
Non-small cell lung cancer cells (A549), mouse monocyte macrophage leukemia cells (RAW264.7), human liver cancer cells (Hep G2), Human gastric mucosal epithelial cells (GES-1) and human normal hepatocytes (L02) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

Lysozyme (hen egg white, ~20,000 U/mg) and GSH were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). N-(1-pyrenyl) maleimide (NPM) and 8-anilino-1-naphtalenesulfonic (ANS) were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer and TCEP was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China). All reagents and solvents were of analytical grade, and ultra pure water (18.3 MΩ·cm) was used in the entire experiment.