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Leupeptin hydrochloride

Manufactured by Merck Group
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Leupeptin hydrochloride is a protease inhibitor used in laboratory research. It functions by inhibiting cysteine, serine, and thiol proteases.

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Lab products found in correlation

Rapamycin, by Merck Group (1 mentions) Bafilomycin a, by Merck Group (1 mentions) Cq diphosphate salt, by Merck Group (1 mentions) Azd8055, by Selleck Chemicals (1 mentions) Sucrose, by Merck Group (1 mentions) Trichloro, by Merck Group (1 mentions) Argatroban monohydrate, by Merck Group (1 mentions) 1 3 butanediol, by Merck Group (1 mentions) Phenylmethanesulfonyl fluoride, by Merck Group (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Streptavidin conjugated cy5, by Rockland Immunochemicals (1 mentions) Phosphate buffered saline tablets, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Jackson ImmunoResearch (1 mentions) Alexa fluor 532, by Thermo Fisher Scientific (1 mentions) Tween 20, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Betaine, by Merck Group (1 mentions)

4 protocols using leupeptin hydrochloride

1

Autophagy Modulators Solubilization

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AZD8055 (Selleck Chem), bafilomycin A (Sigma–Aldrich), and rapamycin (Sigma–Aldrich) were all dissolved in DMSO, while CQ diphosphate salt (Sigma–Aldrich), leupeptin hydrochloride (Sigma–Aldrich), and sucrose (Sigma–Aldrich) were prepared in Milli-Q water. A total of 50× MEM essential amino acids and 100× MEM non-essential amino acids were supplied by Thermo Fisher.
Fedele A.O, & Proud C.G. (2020). Chloroquine and bafilomycin A mimic lysosomal storage disorders and impair mTORC1 signalling. Bioscience Reports, 40(4), BSR20200905.
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2

Fluorescently-Labeled Antibody Detection

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Goat anti-mouse IgG (H + L) labeled with Alexa Fluor 532 and chicken anti-goat IgG (H + L) labeled with Alexa Fluor 647 were purchased from Life Technologies. Cognate antibody pairs and proteins used are listed in SI Table 1. Streptavidin-conjugated Cy5 was obtained from Rockland. Phosphate buffered saline (PBS) tablets and glass slides were purchased from Fisher Scientific. Glycerol, 1,3-butanediol, betaine, trichloro(1H,1H,2H,2H-perfluorooctyl)silane, acetone, hydrochloric acid, Tween-20, argatroban monohydrate, phenylmethanesulfonyl fluoride (PMSF) and leupeptin hydrochloride were purchased from Sigma-Aldrich. Bovine serum albumin (BSA) was purchased from Jackson ImmunoResearch Laboratories, Inc. BSA-free StabilGuard Choice Microarray Stabilizer was purchased from SurModics, Inc. Human alpha-thrombin (HCT-0020), fluorogenic substrate (SN20) and the inhibitor D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK) were purchased from Haematologic Technologies Inc. Nitrocellulose coated slides were purchased from Grace Bio-Laboratories, and aminosilane coated slides were purchased from Schott North America.
Li H., Munzar J.D., Ng A, & Juncker D. (2015). A versatile snap chip for high-density sub-nanoliter chip-to-chip reagent transfer. Scientific Reports, 5, 11688.
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3

Opioid Receptor Binding Assay Protocol

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β-FNA, [3H]β-FNA, naloxone, and U50,488H, were provided by the National Institute on Drug Abuse (Bethsada, MD). [35S]guanosine 5′-O-[γ-thio]triphosphate] (GTPγS) (1250 Ci/mmol), [15, 16-3H]diprenorphine ([3H]DIP) (36–50 Ci/mmol) were purchased from PerkinElmer Life Sciences (Boston, MA); polyethyleneimine, ampicillin, leupeptin hydrochloride, phenylmethylsulfonyl fluoride (PMSF), GDP and GTPγS were purchased from Sigma-Aldrich (St. Louis, MO). Geneticin (G418) was purchased from Cellgro Mediatech, Inc. (Herndon, VA). Lipofectamine 2000 and BamHI were purchased from Invitrogen (Carlsbad, CA). XhoI and dNTPs were purchased from Promega (Madison, WI). Minimal essential medium (MEM), trypsin and penicillin/streptomycin were purchased from Gibco Life Technologies (Grand Island, NY). Bicinchoninic acid (BCA) assay reagents were purchased from Thermo Fisher Scientific, Inc. (Rockford, IL). The following reagents were purchased from the indicated companies: GF/B glass filters, Brandel, Inc. (Gaithersburg, MD); PFU DNA polymerase, Agilent Technologies (Santa Rosa, CA); EcoRI, Roche (Indianapolis, IN).
DiMattio K.M., Chen C., Shi L, & Liu-Chen L.Y. (2014). K3036.58 in the μ opioid (MOP) receptor is important in conferring selectivity for covalent binding of β-funaltrexamine (β-FNA). European journal of pharmacology, 748, 93-100.
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4

Synthesis and Characterization of Organohalogens

Cited 1 time
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Organohalogens were custom synthesized as described in the Supporting Information (SI) or as described previously,21 (link),28 (link) isolated from marine sponges38 (link) or purchased [pyrrole (24), ethyl-4-bromopyrrole-2-carboxylate (27) and 2,3-dibromo-N-methylmaleimide (28)] from SigmaAldrich (St. Louis, MO). Names and structures for the compounds tested are summarized in Table SI1. All the HOCs were dissolved in dry DMSO (10 mM) and stored at −80 °C. Phenylmethylsulfonyl fluoride (PMSF), leupeptin hydrochloride, Mg-ATP, phosphocreatine, creatine phosphokinase, phosphoenolpyruvate, pyruvate kinase/lactic dehydrogenase, Na2ATP, NADH were purchased from Sigma-Aldrich. Arsenazo III was purchased from Santa Cruz Biotechnology (Dallas, TE). Ryanodine was purchased from Ascent Scientific (Cambridge, MA) and dissolved in 100% ethanol and stored at 4 °C until use. [3H]Ryanodine (specific activity: 56.6 Ci/ mmol) was obtained from PerkinElmer (Santa Clara, CA).
Zheng J., McKinnie S.M., El Gamal A., Feng W., Dong Y., Agarwal V., Fenical W., Kumar A., Cao Z., Moore B.S, & Pessah I.N. (2018). Organohalogens Naturally Biosynthesized in Marine Environments and Produced as Disinfection Byproducts Alter Sarco/Endoplasmic Reticulum Ca2+ Dynamics. Environmental science & technology, 52(9), 5469-5478.
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