Vero cells (1.2 × 104 cells) were cultured in DMEM with 1× antibiotic–antimycotic solution and 2% FBS in 384-well black culture plates. The serially diluted compounds and 0.0125 multiplicity of infection (MOI) SARS-CoV-2, 0.05 MOI SARS-CoV, or 0.0625 MOI MERS-CoV were added. At 24 h post-infection, the cells were fixed with 4% paraformaldehyde and stained with antibodies against the SARS-CoV-2 nucleocapsid protein, SARS-CoV spike protein, or MERS-CoV spike protein (Sino Biological Inc., Beijing, China), followed by goat antirabbit IgG secondary antibody and Hoechst 33342 (Thermo Fisher Scientific, Waltham, MA, USA). Images were analyzed using the Operetta® High-Content Imaging System (20×; PerkinElmer, Inc., Waltham, MA, USA) and Image-Mining 3.0 plug-in software [20 (link)].
Sars cov spike protein
Manufactured by Sino Biological
Sourced in China
The SARS-CoV spike protein is a key structural component of the SARS-CoV virus. It plays a critical role in the virus's ability to attach to and infect host cells. The spike protein is an essential tool for research and development related to SARS-CoV and COVID-19.
Automatically generated - may contain errors
2 protocols using sars cov spike protein
1
High-Content Screening of SARS Inhibitors
2
SARS-CoV-2, MERS-CoV, and SARS-CoV Spike Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SARS-CoV-2 spike protein (S1 subunit, His tag) (catalog number: 40591-V08B1), MERS-CoV spike protein (S1 subunit, His tag) (catalog number: 40069-V08B1) and SARS-CoV spike protein (S1 subunit, His tag) (catalog number: 40150-V08B1) were purchased from Sino Biological Inc., China. Proteins were dissolved in an HBS-EP+ buffer for use.
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