Wst 1 cytotoxicity assay reagent

For the cytotoxicity assay, cells were seeded in 96-well culture plastic plates at a density of 1×10³ cells per well. Each well was exposed to radiation at varying doses (0–5 Gy) and the cells were incubated for 72 h, followed by application of the water-soluble tetrazolium (WST)-1 cytotoxicity assay reagent (Roche Diagnostics, Laval, Quebec, Canada) according to the manufacturer's recommendations. Cell viability was assessed by determining the A450 nm of the cell culture media after addition of WST-1 for 2 h. The results are reported as a percentage of the optical density of the untreated control cells, which was designated as 100% cell viability. Percentage of cytotoxicity was calculated as (1-Aexp/Acon) ×100, where Aexp and Acontrol are the absorbance values of the experimental IR-treated and control untreated cells, respectively.

For the cytotoxicity assay to evaluate the proliferation rate, liposarcoma cells were seeded in 96-well culture plastic plates at a density of 1x10³ cells per well. TTFields was added to the dishes and the cells were incubated for 48 h followed by application of the water-soluble tetrazolium (WST)-1 cytotoxicity assay reagent (Roche Diagnostics, Laval, Quebec, Canada: CAS No.150849-52-8) per the manufacturer's recommendations. Cell viability was assessed by determining the A450 nm of the cell culture media after adding WST-1 for 2 h. The results were reported as a percentage of the optical density of the untreated control cells, which was designated as 100% cell viability. Percentage of cytotoxicity was calculated as follows: (1-Aexp/Acontrol) x100; where Aexp and Acontrol are the absorbance values of the experimental drug-treated and control untreated cells, respectively.