All Samples from the resuspended PBMCs or PEMCs were stained with Fc Block (1 μL/100L) (Catalog Number: 554656). The expression of various Treg and Th17 cell markers was measured after immunostaining. According to the manufacturer’s instructions, CD4-Percp-cyTM 5.5 (Catalog Number: 560650) and CD25-BB515 (Catalog Number: 564467) antibodies from BD Biosciences (San Jose, CA, USA) were used for extracellular staining, after which the cells were fixed and permeabilized for intracellular staining with Foxp3-PE (Catalog Number: 320108), Helios-Alexa Fluo 647 (Catalog Number: 563951) and RORγt-Alexa Fluor 647 (Catalog Number: 563620) from BD Biosciences (San Jose, CA, USA). Fluorescence activated cell sorting (FACS) cytometer (Becton Dickinson, USA) was used to detect and collect data for analysis by Cell Quest software.
Helios alexa fluo 647
Manufactured by BD
Sourced in United States
The Helios-Alexa Fluo 647 is a lab equipment product. It is a fluorescent dye that can be used for various applications in biological research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using helios alexa fluo 647
1
Quantification of Treg and Th17 Cells
2
Regulatory and Th17 Cell Phenotyping
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All Samples from the resuspended PBMCs or PEMCs were stained with Fc Block (1 µL/100 µL) (Catalog Number: 554656). The expression of various Treg and Th17 cell markers was measured after immunostaining. According to the manufacturer's instructions, CD4-Percp-cy™ 5.5 (Catalog Number: 560650) and CD25-BB515 (Catalog Number: 564467) antibodies from BD Biosciences (San Jose, CA, USA) were used for extracellular staining, after which the cells were xed and permeabilized for intracellular staining with Foxp3-PE (Catalog Number: 320108), Helios-Alexa Fluo 647 (Catalog Number: 563951) and RORγt-Alexa Fluor 647 (Catalog Number: 563620) from BD Biosciences (San Jose, CA, USA). Fluorescence activated cell sorting (FACS) cytometer (Becton Dickinson, USA ) was used to detect and collect data for analysis by Cell Quest software.
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