Cells were detached by trypsinization, washed with PBS, fixed in 70% ethanol and stored at −20 °C until analysis. Fixed cells were centrifuged (2000 rpm, 5 min) and incubated for 30 min at 37 °C in PBS. After centrifugation, cells were resuspended and stained with propidium iodide using the DNA-Prep Coulter Reagent Kit (Beckman Coulter, Villepinte, France) and were analyzed using an EPICS XL flow cytometer (Beckman Coulter). Computerized gating was applied on the side and forward scattering to exclude small debris and on a pulse width and integral peak of red fluorescence to eliminate aggregates. The data were analyzed by Expo32 acquisition software (Beckman Coulter).
Expo32 acquisition software
Manufactured by Beckman Coulter
The Expo32 acquisition software is a data management tool designed for use with Beckman Coulter's flow cytometry instruments. It provides the core functionality to capture, analyze, and report data generated during flow cytometry experiments.
Automatically generated - may contain errors
2 protocols using expo32 acquisition software
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
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Cells were detached by trypsinization, washed with PBS, fixed in 70% ethanol and stored at −20 °C until analysis. Fixed cells were centrifuged (2000 r.p.m., 5 min) and incubated for 30 min at 37 °C in PBS. After centrifugation, cells were resuspended and stained with propidium iodide using the DNA-Prep Coulter Reagent Kit (Beckman Coulter, Villepinte, France) and were analysed using an EPICS XL flow cytometer (Beckman Coulter). Computerized gating was applied on the side and forward scatter to exclude small debris and on a pulse width and integral peak of red fluorescence to eliminate aggregates. The data were analysed by Expo32 acquisition software (Beckman Coulter).
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