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Safranin o lillie s trichrome

Manufactured by Merck Group
Sourced in United States

Safranin O Lillie's Trichrome is a histological stain used in microscopy. It is a combination of the dyes safranin O and Lillie's trichrome. The stain is used to differentiate between various tissue components, such as nuclei, cytoplasm, and collagen fibers, by displaying them in distinct colors.

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2 protocols using safranin o lillie s trichrome

1

Histological Analysis of Undecalcified Bone Samples

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After sacrifice, bones were fixed overnight at 4°C in 70% ethanol solution and dehydrated in a graded ethanol series. Undecalcified samples were embedded in methyl methacrylate (Merck, Rahway, NJ). Serial sections, 5 μm thick, were cut on a microtome (Polycut E microtome, Leica, Wetzlar, Germany). Series of consecutive sections were stained with Von Kossa (5% silver nitrate solution; Sigma-Aldrich), counterstained with toluidine blue (pH 3.8), and stained with Safranin O Lillie’s Trichrome (Sigma-Aldrich).
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2

Undecalcified Bone Histology

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Bones were fixed overnight at 4°C in 70% ethanol solution and dehydrated in a graded ethanol series. Undecalcified samples were embedded in methyl methacrylate (Merck, Rahway, NJ, United States). Serial sections, 4 μm thick, were cut on a microtome (Polycut E microtome, Leica, Wetzlar, Germany). Series of consecutive sections representative of micro-CT images were stained with von Kossa (5% silver nitrate solution, Sigma-Aldrich, St Louis, MO, United States) and counterstained with toluidine blue (pH 3.8), stained with Masson’s Trichrome (Sigma–Aldrich), Safranin O Lillie’s Trichrome (Sigma–Aldrich).
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