Elisa starter accessory package

Manufactured by Fortis Life Sciences

Sourced in United States

The ELISA Starter Accessory Package is a collection of essential laboratory equipment and accessories for performing enzyme-linked immunosorbent assay (ELISA) techniques. The package includes necessary components such as micropipettes, multichannel pipettes, microplate reader, and other essential accessories to support ELISA experiments.

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Lab products found in correlation

Advia 120, by Bayer (1 mentions)

2 protocols using elisa starter accessory package

Porcine Blood Biomarker Evaluation

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On days 1, 7, 21, and 42, two pigs per pen were selected randomly and were
bled for collecting blood samples via anterior vena cava puncture into
K₃EDTA vacuum tubes or clot activator vacuum tubes (Becton
Dickinson Vacutainer Systems, Franklin Lakes, NJ, USA). The blood samples
were collected during 11:00 to 12:00 h in order to exclude the circadiurnal
fluctuations in hormone concentrations and stored at −4°C.
Blood samples from clot activator vacuum tubes were centrifuged
(3,000×g) for 15 min at 4°C to obtain serum samples and then
stored at −20°C until analysis. The concentrations of blood
urea nitrogen (BUN) and creatinine in serum were analyzed by an automatic
biochemical blood analyzer (HITACHI 747, Hitachi, Tokyo, Japan). The
concentrations of immunoglobulin G (IgG), immunoglobulin A (IgA), and
immunoglobulin M (IgM) in serum were determined using an enzyme-linked
immunosorbent assay kit (ELISA Starter Accessory Package, Bethyl
Laboratories, Montgomery, TX, USA). In addition, whole blood samples from
the K₃EDTA vacuum tube were analyzed for white blood cell (WBC),
red blood cell (RBC), and lymphocyte concentrations using an automatic blood
analyzer (ADVIA 120, Bayer, Tarrytown, NY, USA).

Dang D.X., Cho S, & Kim I.H. (2022). Silybum marianum seed extract supplementation positively affects the body weight of weaned piglets by improving voluntary feed intake. Journal of Animal Science and Technology, 64(4), 696-706.

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Piglet Immune Biomarker Quantification

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The body weight (BW) of piglets and amount of the feed were recorded at 21 d and 24 d of age. The average daily feed intake (ADFI), average daily gain (ADG) and d feed conversion rate (FCR) were calculated during the experiment period. The blood samples were placed into RNAase free tubes and then left to stand at the room temperature for 30 minutes. The blood was centrifuged at 3000 rpm for 15 minutes and then the serum was placed in a 1.5 ml centrifuge tube for the test.
Immunoglobulins (IgA IgG and IgM) and in ammatory factors (TNF-α, IL-6, IL-8 and IL-10) were measured using a pig ELISA quanti cation kit (Bethyl Laboratories, Montgomery, TX, USA) and ELISA starter accessory package (Bethyl Laboratories) according to the manufacturer's instructions. The plates were read at 450 nm with a micro plate reader (Multiskan FC; Thermo Fisher Scienti c, Waltham, MA, USA).

Lang W., Gong H., Li R., Wu M., Chu Q., Wang T., & Zheng X. (2020). Effects of supplemental β-carotene on growth performance, immune function and intestinal mucin in weaned piglets.

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